##################################
# NO CUT ENZYMES ( 45 enzymes) #
##################################
AatII AccIII AclI Aor51HI AscI
BamHI BciVI BseAI BspDI BspEI
BspHI BssHII BstBI ClaI CpoI
DrdI Eco47III EcoRI EcoRV FseI
HindIII KspI MfeI MroI MunI
NaeI NdeI NgoMIV NheI NotI
NruI NspV PacI PmeI Psp1406I
PvuI RcaI RsrII SacII SalI
ScaI SfuI SgrAI SnaBI SwaI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AflII 5282 CTTAAG C^TTAAG
AgeI 4821 ACCGGT A^CCGGT
AhdI 1442 GACGTGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AseI 4119 ATTAAT AT^TAAT
AsnI 4119 ATTAAT AT^TAAT
Asp700I 2749 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1442 GACGTGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2891 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 169 CCTAGG C^CTAGG
BbeI 845 GGCGCC GGCGC^C
BbrPI 2831 CACGTG CAC^GTG
BclI 3423 TGATCA T^GATCA
BfrI 5282 CTTAAG C^TTAAG
BlnI 169 CCTAGG C^CTAGG
BsaBI 1597 GATGAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI 721 CGTCTC CGTCTC(1/5)
Bsp1407I 672 TGTACA T^GTACA
BsrBI 3128 GAGCGG CCGCTC(-3/-3)
BsrFI 4821 ACCGGT [GA]^CCGG[CT]
BsrGI 672 TGTACA T^GTACA
Bst1107I 6005 GTATAC GTA^TAC
BstAPI 859 GCACCAAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I 6005 GTATAC GTA^TAC
Cfr10I 4821 ACCGGT [GA]^CCGG[CT]
EagI 1498 CGGCCG C^GGCCG
Eam1105I 1442 GACGTGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 1498 CGGCCG C^GGCCG
Eco52I 1498 CGGCCG C^GGCCG
EcoT22I 5633 ATGCAT ATGCA^T
FbaI 3423 TGATCA T^GATCA
HpaI 5903 GTTAAC GTT^AAC
KasI 845 GGCGCC G^GCGCC
MamI 1597 GATGAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 246 ACGCGT A^CGCGT
NarI 845 GGCGCC GG^CGCC
NsiI 5633 ATGCAT ATGCA^T
PflFI 2891 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 4821 ACCGGT A^CCGGT
PmaCI 2831 CACGTG CAC^GTG
PmlI 2831 CACGTG CAC^GTG
PshBI 4119 ATTAAT AT^TAAT
SapI 2536 GCTCTTC GCTCTTC(1/4)
SbfI 5754 CCTGCAGG CCTGCA^GG
SfiI 2515 GGCCCTGGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 845 GGCGCC GGC^GCC
SpeI 5172 ACTAGT A^CTAGT
Sse8387I 5754 CCTGCAGG CCTGCA^GG
SspBI 672 TGTACA T^GTACA
Tth111I 2891 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 3894 TCTAGA T^CTAGA
XmnI 2749 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
Acc65I 1722 GGTACC G^GTACC
2874 GGTACC
AccI 5168 GTAGAC GT^[AC][GT]AC
6005 GTATAC
AcsI 5241 GAATTT [GA]^AATT[CT]
5950 AAATTT
AcyI 845 GGCGCC G[GA]^CG[CT]C
1702 GGCGTC
Alw44I 1505 GTGCAC G^TGCAC
5516 GTGCAC
ApaLI 1505 GTGCAC G^TGCAC
5516 GTGCAC
ApoI 5241 GAATTT [GA]^AATT[CT]
5950 AAATTT
Asp718I 1722 GGTACC G^GTACC
2874 GGTACC
BbvCI 2263 GCTGAGG CCTCAGC(-5/-2)
3448 GCTGAGG
BcgI 242 CGACACGCGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2082 GCATCCTGCTCG
BsaHI 845 GGCGCC G[GA]^CG[CT]C
1702 GGCGTC
BsiEI 1498 CGGCCG CG[GA][CT]^CG
1880 CGGTCG
BsiWI 1545 CGTACG C^GTACG
1896 CGTACG
BspLU11I 1323 ACATGT A^CATGT
4210 ACATGT
BstEII 1034 GGTGACC G^GT[ACGT]ACC
3361 GGTTACC
BstPI 1034 GGTGACC G^GT[ACGT]ACC
3361 GGTTACC
DraI 4106 TTTAAA TTT^AAA
5785 TTTAAA
EcoO65I 1034 GGTGACC G^GT[ACGT]ACC
3361 GGTTACC
Hin1I 845 GGCGCC G[GA]^CG[CT]C
1702 GGCGTC
KpnI 1722 GGTACC GGTAC^C
2874 GGTACC
PaeR7I 1727 CTCGAG C^TCGAG
2197 CTCGAG
PshAI 1795 GACCCACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
4222 GACTACTGTC
SacI 1181 GAGCTC GAGCT^C
3014 GAGCTC
SspI 5558 AATATT AAT^ATT
6092 AATATT
XhoI 1727 CTCGAG C^TCGAG
2197 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 137 enzymes) #
#########################################
AccII AciI AfaI AflIII AluI
AlwI AlwNI AocI ApaI AspHI
AvaI AvaII AviII BalI BanI
BanII BbsI BbvI BcnI BfaI
BglI BglII BlpI BmyI BpmI
Bpu1102I BpuAI BsaAI BsaI BsaJI
BsaWI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI BsoBI
Bsp1286I BspMI BsrDI BsrI BssKI
BssSI BstF5I BstNI BstUI BstXI
BstYI Bsu36I Cac8I CelII CfoI
Cfr13I DdeI DpnII DraII DraIII
DsaI EaeI EarI Eco81I EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI FspI HaeII HaeIII HapII
HgaI HhaI HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PflMI PleI PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SexAI SfaNI SfcI SmaI
SmlI SphI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII
XmaCI XmaI