##################################
# NO CUT ENZYMES ( 92 enzymes) #
##################################
Acc65I AccI AccIII AclI AflII
AgeI AhdI AlwNI Aor51HI AscI
AseI AsnI Asp718I AspEI AspI
AviII AvrII BbeI BbrPI BciVI
BclI BfrI BlnI BsaAI BsaBI
BsaWI BseAI BsiWI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrDI
BsrGI BssHII Bst1107I BstBI BstEII
BstPI BstZ17I ClaI CpoI DraI
Eam1105I Eco47III EcoO65I EcoT22I FbaI
FseI FspI HpaI KasI KpnI
MamI MfeI MluI MroI MslI
MunI NarI NdeI NotI NruI
NsiI NspV PacI PflFI PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI RcaI RsrII SalI ScaI
SfiI SfoI SfuI SnaBI SpeI
SphI SspBI SspI StuI SwaI
Tth111I XbaI
###################################
# ONE CUT ENZYMES ( 31 enzymes) #
###################################
AflIII 1356 ACGTGT A^C[GA][CT]GT
BalI 1332 TGGCCA TGG^CCA
BamHI 171 GGATCC G^GATCC
BbsI 280 GAAGAC GAAGAC(2/6)
BcgI 2096 GCAGCCAAGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 2478 AGATCT A^GATCT
BpuAI 280 GAAGAC GAAGAC(2/6)
BsmBI 706 CGTCTC CGTCTC(1/5)
BstAPI 256 GCAGTACATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DraIII 1670 CACAAAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 2779 GACCCTCGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 2550 GAATTC G^AATTC
EcoRV 2446 GATATC GAT^ATC
HincII 2739 GTCAAC GT[CT]^[GA]AC
HindII 2739 GTCAAC GT[CT]^[GA]AC
MluNI 1332 TGGCCA TGG^CCA
MscI 1332 TGGCCA TGG^CCA
NheI 2116 GCTAGC G^CTAGC
NspI 261 ACATGC [GA]CATG^[CT]
PaeR7I 2671 CTCGAG C^TCGAG
PflMI 319 CCAGCCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1011 AGGACCT [GA]G^G[AT]CC[CT]
PshAI 715 GACGCCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI 1476 GCTCTTC GCTCTTC(1/4)
SbfI 2989 CCTGCAGG CCTGCA^GG
SexAI 2658 ACCTGGT A^CC[AT]GGT
SgrAI 1404 CACCGGCG C[GA]^CCGG[CT]G
SmlI 2671 CTCGAG C^T[CT][GA]AG
Sse8387I 2989 CCTGCAGG CCTGCA^GG
Van91I 319 CCAGCCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 2671 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AatII 54 GACGTC GACGT^C
895 GACGTC
Alw44I 1556 GTGCAC G^TGCAC
1925 GTGCAC
AlwI 172 GATCC GGATC(4/5)
171 GGATC
AocI 1093 CCTCAGG CC^T[ACGT]AGG
2223 CCTCAGG
ApaLI 1556 GTGCAC G^TGCAC
1925 GTGCAC
Asp700I 2065 GAAACCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2680 GAATTTTTTC
BanI 1030 GGTGCC G^G[CT][GA]CC
1402 GGCACC
BbvCI 1210 CCTCAGC CCTCAGC(-5/-2)
2817 GCTGAGG
BfaI 407 CTAG C^TAG
2117 CTAG
BsaI 2843 GAGACC GGTCTC(1/5)
214 GGTCTC
BsgI 1172 CTGCAC GTGCAG(16/14)
2416 GTGCAG
BstXI 957 CCAAAGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1518 CCACCAGGCTGG
BstYI 171 GGATCC [GA]^GATC[CT]
2478 AGATCT
Bsu36I 1093 CCTCAGG CC^T[ACGT]AGG
2223 CCTCAGG
DpnII 172 GATC ^GATC
2479 GATC
Eco81I 1093 CCTCAGG CC^T[ACGT]AGG
2223 CCTCAGG
EcoNI 1576 CCTGTCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1950 CCTCGCTCAGG
HindIII 519 AAGCTT A^AGCTT
3010 AAGCTT
KspI 165 CCGCGG CCGC^GG
1422 CCGCGG
MaeI 407 CTAG C^TAG
2117 CTAG
MboI 172 GATC ^GATC
2479 GATC
MflI 171 GGATCC [GA]^GATC[CT]
2478 AGATCT
NaeI 613 GCCGGC GCC^GGC
1775 GCCGGC
NdeII 172 GATC ^GATC
2479 GATC
NgoMIV 613 GCCGGC G^CCGGC
1775 GCCGGC
SacII 165 CCGCGG CCGC^GG
1422 CCGCGG
Sau3AI 172 GATC ^GATC
2479 GATC
SmaI 184 CCCGGG CCC^GGG
1901 CCCGGG
TfiI 2614 GATTC G^A[AT]TC
2637 GAATC
XhoII 171 GGATCC [GA]^GATC[CT]
2478 AGATCT
XmaCI 184 CCCGGG C^CCGGG
1901 CCCGGG
XmaI 184 CCCGGG C^CCGGG
1901 CCCGGG
XmnI 2065 GAAACCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2680 GAATTTTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 102 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI ApaI ApoI AspHI AvaI
AvaII BanII BbvI BcnI BglI
BlpI BmyI BpmI Bpu1102I BsaHI
BsaJI BseRI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrFI BsrI BssKI
BssSI BstF5I BstNI BstUI Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DraII DsaI EaeI EagI EarI
EclXI Eco52I EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeII MaeIII MboII MnlI MseI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NlaIII NlaIV PleI
PstI PvuII RsaI SacI Sau96I
ScrFI SfaNI SfcI StyI TaqI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XcmI