##################################
# NO CUT ENZYMES ( 60 enzymes) #
##################################
AatII Alw44I Aor51HI ApaLI AscI
AviII BamHI BglII BlpI Bpu1102I
BsaBI BsiWI BsmBI BsmI BspDI
BssHII Bst1107I BstEII BstPI BstZ17I
CelII ClaI CpoI Eco47III EcoNI
EcoO65I EcoRV EcoT22I FseI FspI
MamI MfeI MluI MunI NcoI
NotI NruI NsiI PacI PaeR7I
PmeI PshAI PvuI RsrII SalI
SbfI SexAI SfiI SgrAI SmaI
SnaBI SpeI SphI Sse8387I StuI
SwaI XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 69 enzymes) #
###################################
Acc65I 2959 GGTACC G^GTACC
AccIII 1497 TCCGGA T^CCGGA
AclI 372 AACGTT AA^CGTT
AcyI 1463 GGCGCC G[GA]^CG[CT]C
AflII 420 CTTAAG C^TTAAG
AgeI 3681 ACCGGT A^CCGGT
AhdI 882 GACTCACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 1378 CCTCAGG CC^T[ACGT]AGG
ApaI 1480 GGGCCC GGGCC^C
Asp700I 1179 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 2959 GGTACC G^GTACC
AspEI 882 GACTCACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1665 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 576 CCTAGG C^CTAGG
BbeI 1463 GGCGCC GGCGC^C
BcgI 1088 CGAAGATTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2909 GTATCC GTATCC(6/5)
BfrI 420 CTTAAG C^TTAAG
BglI 1455 GCCTCCGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 576 CCTAGG C^CTAGG
BsaHI 1463 GGCGCC G[GA]^CG[CT]C
BseAI 1497 TCCGGA T^CCGGA
BseRI 2493 GAGGAG GAGGAG(10/8)
BsiEI 1446 CGGCCG CG[GA][CT]^CG
BsmFI 3043 GGGAC GGGAC(10/14)
Bsp1407I 1219 TGTACA T^GTACA
BspEI 1497 TCCGGA T^CCGGA
BspHI 1061 TCATGA T^CATGA
BspMI 3068 GCAGGT ACCTGC(4/8)
BsrBI 341 GAGCGG CCGCTC(-3/-3)
BsrGI 1219 TGTACA T^GTACA
BssSI 3010 CTCGTG CACGAG(-5/-1)
BstAPI 2933 GCAGACACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 308 TTCGAA TT^CGAA
BstXI 2097 CCAAGTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1378 CCTCAGG CC^T[ACGT]AGG
DrdI 1662 GACGACGGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 1446 CGGCCG C^GGCCG
Eam1105I 882 GACTCACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 1446 CGGCCG C^GGCCG
Eco52I 1446 CGGCCG C^GGCCG
Eco81I 1378 CCTCAGG CC^T[ACGT]AGG
EcoRI 2325 GAATTC G^AATTC
Hin1I 1463 GGCGCC G[GA]^CG[CT]C
HpaI 298 GTTAAC GTT^AAC
KasI 1463 GGCGCC G^GCGCC
KpnI 2959 GGTACC GGTAC^C
KspI 1653 CCGCGG CCGC^GG
MroI 1497 TCCGGA T^CCGGA
NaeI 2440 GCCGGC GCC^GGC
NarI 1463 GGCGCC GG^CGCC
NdeI 286 CATATG CA^TATG
NgoMIV 2440 GCCGGC G^CCGGC
NheI 1451 GCTAGC G^CTAGC
NspV 308 TTCGAA TT^CGAA
PflFI 1665 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 63 CCAGCCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 3681 ACCGGT A^CCGGT
Psp1406I 372 AACGTT AA^CGTT
PvuII 793 CAGCTG CAG^CTG
RcaI 1061 TCATGA T^CATGA
SacII 1653 CCGCGG CCGC^GG
SfoI 1463 GGCGCC GGC^GCC
SfuI 308 TTCGAA TT^CGAA
SspBI 1219 TGTACA T^GTACA
Tth111I 1665 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 63 CCAGCCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1394 CCAAGAAAGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 1179 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccI 781 GTCTAC GT^[AC][GT]AC
2195 GTCTAC
AseI 691 ATTAAT AT^TAAT
2302 ATTAAT
AsnI 691 ATTAAT AT^TAAT
2302 ATTAAT
BbrPI 1897 CACGTG CAC^GTG
2789 CACGTG
BbvCI 1595 CCTCAGC CCTCAGC(-5/-2)
2002 CCTCAGC
BsaAI 1897 CACGTG [CT]AC^GT[GA]
2789 CACGTG
BsaI 2027 GGTCTC GGTCTC(1/5)
2901 GGTCTC
BsaWI 1497 TCCGGA [AT]^CCGG[AT]
3681 ACCGGT
BspLU11I 4 ACATGT A^CATGT
302 ACATGT
BsrDI 2429 CATTGC GCAATG(2/0)
2996 GCAATG
BsrFI 2440 GCCGGC [GA]^CCGG[CT]
3681 ACCGGT
Cfr10I 2440 GCCGGC [GA]^CCGG[CT]
3681 ACCGGT
DraII 1382 AGGACCC [GA]G^G[ACGT]CC[CT]
1855 AGGACCT
DraIII 173 CACTAAGTG CAC[ACGT][ACGT][ACGT]^GTG
760 CACAAAGTG
DsaI 68 CCGTGG C^C[GA][CT]GG
1653 CCGCGG
EcoO109I 1382 AGGACCC [GA]G^G[ACGT]CC[CT]
1855 AGGACCT
EcoT14I 576 CCTAGG C^C[AT][AT]GG
1403 CCTTGG
HaeII 1463 GGCGCC [GA]GCGC^[CT]
2399 AGCGCC
HindIII 115 AAGCTT A^AGCTT
1552 AAGCTT
PmaCI 1897 CACGTG CAC^GTG
2789 CACGTG
PmlI 1897 CACGTG CAC^GTG
2789 CACGTG
PpuMI 1382 AGGACCC [GA]G^G[AT]CC[CT]
1855 AGGACCT
PshBI 691 ATTAAT AT^TAAT
2302 ATTAAT
SapI 718 GAAGAGC GCTCTTC(1/4)
1182 GCTCTTC
ScaI 3581 AGTACT AGT^ACT
3662 AGTACT
StyI 576 CCTAGG C^C[AT][AT]GG
1403 CCTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 103 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApoI AspHI
AvaI AvaII BalI BanI BanII
BbsI BbvI BclI BcnI BfaI
BmyI BpmI BpuAI BsaJI BsgI
BsiHKAI BsiYI BslI BsmAI BsoBI
Bsp1286I BsrI BssKI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraI EaeI EarI
EcoRII FauI FbaI Fnu4HI FokI
HaeIII HapII HgaI HhaI HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NspI PleI PstI
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII