##################################
# NO CUT ENZYMES ( 73 enzymes) #
##################################
AatII Acc65I AcyI AgeI Alw44I
AocI Aor51HI ApaLI AscI Asp718I
AviII AvrII BbeI BcgI BlnI
BsaHI BsaI BsiEI BsiWI BsmBI
Bsp1407I BspDI BsrBI BsrGI BssHII
BssSI BstBI BstEII BstPI Bsu36I
ClaI DrdI EagI EclXI Eco47III
Eco52I Eco81I EcoO65I EcoRV FseI
FspI HaeII Hin1I KasI KpnI
KspI MluI NaeI NarI NdeI
NgoMIV NheI NotI NruI NspV
PacI PinAI PmeI PvuI SacII
SbfI SfoI SfuI SgrAI SmaI
SnaBI SphI Sse8387I SspBI SspI
SwaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AccII 19 CGCG CG^CG
AflII 5996 CTTAAG C^TTAAG
AflIII 6176 ACATGT A^C[GA][CT]GT
AhdI 2661 GACCTGCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 174 GGGCCC GGGCC^C
AspEI 2661 GACCTGCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 5369 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 5220 TGGCCA TGG^CCA
BbrPI 2487 CACGTG CAC^GTG
BciVI 3346 GGATAC GTATCC(6/5)
BfrI 5996 CTTAAG C^TTAAG
BglI 3136 GCCAAGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 1052 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1052 GCTGAGC GC^T[ACGT]AGC
BsaAI 2487 CACGTG [CT]AC^GT[GA]
BsmI 5750 GAATGC GAATGC(1/-1)
BspLU11I 6176 ACATGT A^CATGT
BsrFI 117 ACCGGC [GA]^CCGG[CT]
Bst1107I 4209 GTATAC GTA^TAC
BstUI 19 CGCG CG^CG
BstZ17I 4209 GTATAC GTA^TAC
CelII 1052 GCTGAGC GC^T[ACGT]AGC
Cfr10I 117 ACCGGC [GA]^CCGG[CT]
CpoI 33 CGGACCG CG^G[AT]CCG
DraIII 759 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 5220 TGGCCA [CT]^GGCC[GA]
Eam1105I 2661 GACCTGCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 1820 GAATTC G^AATTC
HgaI 818 GCGTC GACGC(5/10)
HpaI 4630 GTTAAC GTT^AAC
MluNI 5220 TGGCCA TGG^CCA
MscI 5220 TGGCCA TGG^CCA
MslI 4177 CATGACTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 19 CGCG CG^CG
PflFI 5369 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 3698 CCACTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 2487 CACGTG CAC^GTG
PmlI 2487 CACGTG CAC^GTG
RsrII 33 CGGACCG CG^G[AT]CCG
SacI 1055 GAGCTC GAGCT^C
SalI 1161 GTCGAC G^TCGAC
SexAI 760 ACCAGGT A^CC[AT]GGT
SfiI 3135 GGCCAAGAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
Tth111I 5369 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 3698 CCACTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 73 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AclI 3414 AACGTT AA^CGTT
6282 AACGTT
AseI 5215 ATTAAT AT^TAAT
6244 ATTAAT
AsnI 5215 ATTAAT AT^TAAT
6244 ATTAAT
AvaI 315 CTCGAG C^[CT]CG[GA]G
2565 CTCGAG
BclI 5764 TGATCA T^GATCA
6268 TGATCA
BsaBI 2958 GATCGGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5640 GATTTGAATC
BsoBI 315 CTCGAG C^[CT]CG[GA]G
2565 CTCGAG
EcoNI 5148 CCTTTGGTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
5882 CCTGTTGGAGG
FauI 1787 GCGGG CCCGC(4/6)
3311 GCGGG
FbaI 5764 TGATCA T^GATCA
6268 TGATCA
HindIII 1317 AAGCTT A^AGCTT
3238 AAGCTT
MamI 2958 GATCGGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5640 GATTTGAATC
NcoI 2967 CCATGG C^CATGG
6254 CCATGG
PaeR7I 315 CTCGAG C^TCGAG
2565 CTCGAG
PpuMI 2659 AGGACCT [GA]G^G[AT]CC[CT]
4929 AGGACCT
PshAI 5045 GACTTGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
5917 GACAATTGTC
PshBI 5215 ATTAAT AT^TAAT
6244 ATTAAT
Psp1406I 3414 AACGTT AA^CGTT
6282 AACGTT
ScaI 3150 AGTACT AGT^ACT
5673 AGTACT
StuI 1731 AGGCCT AGG^CCT
5759 AGGCCT
XhoI 315 CTCGAG C^TCGAG
2565 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 118 enzymes) #
#########################################
AccI AccIII AciI AcsI AfaI
AluI AlwI AlwNI ApoI Asp700I
AspHI AvaII BamHI BanI BanII
BbsI BbvCI BbvI BcnI BfaI
BglII BmyI BpmI BpuAI BsaJI
BsaWI BseAI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspEI BspHI BspMI BsrDI BsrI
BssKI BstAPI BstF5I BstNI BstXI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraI DraII DsaI EarI
EcoO109I EcoRII EcoT14I EcoT22I Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MfeI MflI MnlI
MroI MseI MspA1I MspI MunI
MvaI MwoI NciI NdeII NlaIII
NlaIV NsiI NspI PleI PstI
PvuII RcaI RsaI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SpeI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XcmI XhoII XmnI