##################################
# NO CUT ENZYMES ( 54 enzymes) #
##################################
AclI AgeI Aor51HI AscI AseI
AsnI BamHI BsiWI Bsp1407I BspDI
BsrGI BssHII Bst1107I BstBI BstZ17I
ClaI CpoI DrdI EagI EclXI
Eco47III Eco52I EcoT22I FseI HincII
HindII HpaI MluI NaeI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PinAI PmeI PshBI
Psp1406I PvuI RsrII SalI ScaI
SexAI SfiI SfuI SgrAI SnaBI
SpeI SphI SspBI StuI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AatII 3867 GACGTC GACGT^C
AccIII 582 TCCGGA T^CCGGA
AflII 3462 CTTAAG C^TTAAG
AhdI 4552 GACACTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 4652 CCTGAGG CC^T[ACGT]AGG
AspEI 4552 GACACTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 5235 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1926 TGCGCA TGC^GCA
AvrII 5106 CCTAGG C^CTAGG
BbeI 817 GGCGCC GGCGC^C
BcgI 2538 CGATGGGCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 3462 CTTAAG C^TTAAG
BlnI 5106 CCTAGG C^CTAGG
BlpI 4191 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 4191 GCTCAGC GC^T[ACGT]AGC
BsaWI 582 TCCGGA [AT]^CCGG[AT]
BseAI 582 TCCGGA T^CCGGA
BsiEI 2291 CGGTCG CG[GA][CT]^CG
BspEI 582 TCCGGA T^CCGGA
BsrFI 505 GCCGGT [GA]^CCGG[CT]
BssSI 4690 CTCGTG CACGAG(-5/-1)
BstEII 1201 GGTAACC G^GT[ACGT]ACC
BstPI 1201 GGTAACC G^GT[ACGT]ACC
Bsu36I 4652 CCTGAGG CC^T[ACGT]AGG
CelII 4191 GCTCAGC GC^T[ACGT]AGC
Cfr10I 505 GCCGGT [GA]^CCGG[CT]
DraIII 5000 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 4552 GACACTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 4652 CCTGAGG CC^T[ACGT]AGG
EcoO65I 1201 GGTAACC G^GT[ACGT]ACC
EcoRV 1781 GATATC GAT^ATC
FspI 1926 TGCGCA TGC^GCA
HgaI 2428 GACGC GACGC(5/10)
HindIII 3737 AAGCTT A^AGCTT
KasI 817 GGCGCC G^GCGCC
MfeI 4511 CAATTG C^AATTG
MroI 582 TCCGGA T^CCGGA
MunI 4511 CAATTG C^AATTG
NarI 817 GGCGCC GG^CGCC
PaeR7I 4624 CTCGAG C^TCGAG
PflFI 5235 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
SacI 3500 GAGCTC GAGCT^C
SbfI 4603 CCTGCAGG CCTGCA^GG
SfoI 817 GGCGCC GGC^GCC
Sse8387I 4603 CCTGCAGG CCTGCA^GG
SspI 3149 AATATT AAT^ATT
SwaI 4417 ATTTAAAT ATTT^AAAT
Tth111I 5235 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 844 TCTAGA T^CTAGA
XhoI 4624 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
Acc65I 830 GGTACC G^GTACC
2400 GGTACC
AccI 1886 GTCTAC GT^[AC][GT]AC
3366 GTCTAC
AcyI 817 GGCGCC G[GA]^CG[CT]C
3867 GACGTC
Alw44I 1148 GTGCAC G^TGCAC
4693 GTGCAC
ApaI 2973 GGGCCC GGGCC^C
5138 GGGCCC
ApaLI 1148 GTGCAC G^TGCAC
4693 GTGCAC
Asp700I 1981 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3616 GAAGCACTTC
Asp718I 830 GGTACC G^GTACC
2400 GGTACC
BbvCI 2635 GCTGAGG CCTCAGC(-5/-2)
5168 GCTGAGG
BciVI 525 GGATAC GTATCC(6/5)
3192 GTATCC
BglII 4182 AGATCT A^GATCT
5029 AGATCT
BsaBI 206 GATTATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3308 GATTAAAATC
BsaHI 817 GGCGCC G[GA]^CG[CT]C
3867 GACGTC
BspLU11I 2115 ACATGT A^CATGT
5541 ACATGT
BsrBI 786 GAGCGG CCGCTC(-3/-3)
2252 GAGCGG
BsrDI 3384 CATTGC GCAATG(2/0)
5385 CATTGC
DraI 4044 TTTAAA TTT^AAA
4418 TTTAAA
EcoRI 1315 GAATTC G^AATTC
4680 GAATTC
HaeII 817 GGCGCC [GA]GCGC^[CT]
2348 AGCGCC
Hin1I 817 GGCGCC G[GA]^CG[CT]C
3867 GACGTC
KpnI 830 GGTACC GGTAC^C
2400 GGTACC
KspI 1969 CCGCGG CCGC^GG
2396 CCGCGG
MamI 206 GATTATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3308 GATTAAAATC
PshAI 1251 GACAGATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1879 GACCTACGTC
SacII 1969 CCGCGG CCGC^GG
2396 CCGCGG
SapI 634 GCTCTTC GCTCTTC(1/4)
1408 GCTCTTC
SmaI 2049 CCCGGG CCC^GGG
2977 CCCGGG
XmaCI 2049 CCCGGG C^CCGGG
2977 CCCGGG
XmaI 2049 CCCGGG C^CCGGG
2977 CCCGGG
XmnI 1981 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3616 GAAGCACTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 124 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApoI AspHI
AvaI AvaII BalI BanI BanII
BbrPI BbsI BbvI BclI BcnI
BfaI BglI BmyI BpmI BpuAI
BsaAI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsmI BsoBI Bsp1286I BspHI
BspMI BsrI BssKI BstAPI BstF5I
BstNI BstUI BstXI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoNI EcoO109I
EcoRII EcoT14I FauI FbaI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PflMI PleI PmaCI
PmlI PpuMI PstI PvuII RcaI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII