##################################
# NO CUT ENZYMES ( 80 enzymes) #
##################################
Acc65I AccIII AflII AgeI AhdI
Alw44I ApaLI AscI AseI AsnI
Asp718I AspEI AspI AviII AvrII
BalI BamHI BbsI BcgI BciVI
BfrI BglII BlnI BpuAI BseAI
BsiEI BsiWI BspDI BspEI BssHII
Bst1107I BstBI BstEII BstPI BstZ17I
ClaI CpoI EagI Eam1105I EclXI
Eco52I EcoO65I EcoRI EcoRV FseI
FspI HindIII HpaI KpnI MluNI
MroI MscI NcoI NheI NotI
NruI NspV PacI PaeR7I PflFI
PinAI PmeI PshAI PshBI PvuI
RsrII SalI SbfI ScaI SfuI
SgrAI SnaBI SpeI Sse8387I SspI
StuI SwaI Tth111I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
AatII 1927 GACGTC GACGT^C
AclI 2744 AACGTT AA^CGTT
AocI 3272 CCTGAGG CC^T[ACGT]AGG
Aor51HI 2088 AGCGCT AGC^GCT
BbeI 1812 GGCGCC GGCGC^C
BbrPI 1828 CACGTG CAC^GTG
BbvCI 2253 CCTCAGC CCTCAGC(-5/-2)
BclI 669 TGATCA T^GATCA
BsaAI 1828 CACGTG [CT]AC^GT[GA]
BsaBI 778 GATCCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1391 TCCGGT [AT]^CCGG[AT]
BsmI 1585 GAATGC GAATGC(1/-1)
Bsp1407I 3042 TGTACA T^GTACA
BspHI 2094 TCATGA T^CATGA
BsrFI 2575 GCCGGC [GA]^CCGG[CT]
BsrGI 3042 TGTACA T^GTACA
BstXI 1489 CCATCTGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 3272 CCTGAGG CC^T[ACGT]AGG
Cfr10I 2575 GCCGGC [GA]^CCGG[CT]
DraIII 2013 CACGGAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 3065 GACAATTGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 2088 AGCGCT AGC^GCT
Eco81I 3272 CCTGAGG CC^T[ACGT]AGG
EcoNI 1411 CCTTGTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 2458 ATGCAT ATGCA^T
FbaI 669 TGATCA T^GATCA
KasI 1812 GGCGCC G^GCGCC
MamI 778 GATCCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 2425 ACGCGT A^CGCGT
NaeI 2575 GCCGGC GCC^GGC
NarI 1812 GGCGCC GG^CGCC
NdeI 2894 CATATG CA^TATG
NgoMIV 2575 GCCGGC G^CCGGC
NsiI 2458 ATGCAT ATGCA^T
PflMI 2378 CCAGACTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1828 CACGTG CAC^GTG
PmlI 1828 CACGTG CAC^GTG
PpuMI 680 GGGACCC [GA]G^G[AT]CC[CT]
Psp1406I 2744 AACGTT AA^CGTT
RcaI 2094 TCATGA T^CATGA
SapI 1060 GAAGAGC GCTCTTC(1/4)
SexAI 2133 ACCTGGT A^CC[AT]GGT
SfiI 2569 GGCCCTGCCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 1812 GGCGCC GGC^GCC
SmaI 1799 CCCGGG CCC^GGG
SmlI 3162 CTTGAG C^T[CT][GA]AG
SphI 2035 GCATGC GCATG^C
SspBI 3042 TGTACA T^GTACA
Van91I 2378 CCAGACTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 781 CCAGATCATCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 1799 CCCGGG C^CCGGG
XmaI 1799 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AccI 1373 GTCTAC GT^[AC][GT]AC
3074 GTCTAC
ApaI 990 GGGCCC GGGCC^C
2210 GGGCCC
AvaI 1799 CCCGGG C^[CT]CG[GA]G
2623 CTCGGG
BfaI 42 CTAG C^TAG
2914 CTAG
BglI 1592 GCCCAGTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2570 GCCCTGCCGGC
BsaI 1124 GAGACC GGTCTC(1/5)
1790 GAGACC
BsmBI 1109 GAGACG CGTCTC(1/5)
2407 GAGACG
BsoBI 1799 CCCGGG C^[CT]CG[GA]G
2623 CTCGGG
BspLU11I 434 ACATGT A^CATGT
3081 ACATGT
BssSI 2360 CACGAG CACGAG(-5/-1)
968 CTCGTG
DsaI 503 CCGCGG C^C[GA][CT]GG
1131 CCGCGG
HincII 919 GTTGAC GT[CT]^[GA]AC
959 GTCAAC
HindII 919 GTTGAC GT[CT]^[GA]AC
959 GTCAAC
KspI 503 CCGCGG CCGC^GG
1131 CCGCGG
MaeI 42 CTAG C^TAG
2914 CTAG
MfeI 233 CAATTG C^AATTG
3067 CAATTG
MslI 886 CACCCCCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1490 CATCTGAGTG
MunI 233 CAATTG C^AATTG
3067 CAATTG
SacI 2024 GAGCTC GAGCT^C
2363 GAGCTC
SacII 503 CCGCGG CCGC^GG
1131 CCGCGG
#########################################
# 3 OR MORE CUT ENZYMES ( 106 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI AlwNI ApoI
Asp700I AspHI AvaII BanI BanII
BbvI BcnI BlpI BmyI BpmI
Bpu1102I BsaHI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BspMI BsrBI BsrDI BsrI
BssKI BstAPI BstF5I BstNI BstUI
BstYI Cac8I CelII CfoI Cfr13I
DdeI DpnII DraI DraII EaeI
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeII
MaeIII MboI MboII MflI MnlI
MseI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PleI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII
XmnI