##################################
# NO CUT ENZYMES ( 87 enzymes) #
##################################
AatII AccI AccIII AclI AgeI
AhdI Alw44I AocI Aor51HI ApaLI
AscI AseI AsnI AspEI AviII
BamHI BbsI BbvCI BcgI BciVI
BclI BpuAI BsaBI BseAI BsiEI
BsiWI Bsp1407I BspDI BspEI BspLU11I
BsrBI BsrGI BssHII Bst1107I BstBI
BstEII BstPI BstZ17I Bsu36I ClaI
CpoI DraI DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRV EcoT22I FbaI FseI FspI
HpaI MamI MfeI MluI MroI
MunI NdeI NheI NotI NruI
NsiI NspI NspV PacI PinAI
PmeI PshBI Psp1406I PvuI RsrII
SalI SbfI ScaI SexAI SfuI
SgrAI SnaBI SphI Sse8387I SspBI
SspI SwaI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
Acc65I 682 GGTACC G^GTACC
AflII 962 CTTAAG C^TTAAG
AflIII 911 ACACGT A^C[GA][CT]GT
Asp700I 1736 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 682 GGTACC G^GTACC
AspHI 8 GAGCTC G[AT]GC[AT]^C
AspI 2247 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 16 CCTAGG C^CTAGG
BfrI 962 CTTAAG C^TTAAG
BglII 1636 AGATCT A^GATCT
BlnI 16 CCTAGG C^CTAGG
BsaWI 1558 TCCGGT [AT]^CCGG[AT]
BsiHKAI 8 GAGCTC G[AT]GC[AT]^C
BsmI 1507 GCATTC GAATGC(1/-1)
BspHI 2390 TCATGA T^CATGA
BspMI 129 ACCTGC ACCTGC(4/8)
BsrFI 820 GCCGGC [GA]^CCGG[CT]
BssSI 883 CACGAG CACGAG(-5/-1)
BstXI 2221 CCATCGCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 820 GCCGGC [GA]^CCGG[CT]
EcoRI 875 GAATTC G^AATTC
HincII 1980 GTCAAC GT[CT]^[GA]AC
HindII 1980 GTCAAC GT[CT]^[GA]AC
HindIII 959 AAGCTT A^AGCTT
KpnI 682 GGTACC GGTAC^C
KspI 68 CCGCGG CCGC^GG
NaeI 820 GCCGGC GCC^GGC
NgoMIV 820 GCCGGC G^CCGGC
PflFI 2247 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
RcaI 2390 TCATGA T^CATGA
SacI 8 GAGCTC GAGCT^C
SacII 68 CCGCGG CCGC^GG
SapI 2487 GCTCTTC GCTCTTC(1/4)
SmaI 1303 CCCGGG CCC^GGG
SpeI 1592 ACTAGT A^CTAGT
StuI 1708 AGGCCT AGG^CCT
Tth111I 2247 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 479 TCTAGA T^CTAGA
XmaCI 1303 CCCGGG C^CCGGG
XmaI 1303 CCCGGG C^CCGGG
XmnI 1736 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AcsI 432 AAATTC [GA]^AATT[CT]
875 GAATTC
AfaI 683 GTAC GT^AC
1088 GTAC
ApoI 432 AAATTC [GA]^AATT[CT]
875 GAATTC
BbrPI 912 CACGTG CAC^GTG
1404 CACGTG
BlpI 815 GCTGAGC GC^T[ACGT]AGC
2375 GCTGAGC
Bpu1102I 815 GCTGAGC GC^T[ACGT]AGC
2375 GCTGAGC
BsmBI 1125 CGTCTC CGTCTC(1/5)
2316 GAGACG
BsrDI 1262 CATTGC GCAATG(2/0)
1435 GCAATG
BstAPI 651 GCAGGAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1192 GCAAGTCCTGC
CelII 815 GCTGAGC GC^T[ACGT]AGC
2375 GCTGAGC
DraIII 1404 CACGTGGTG CAC[ACGT][ACGT][ACGT]^GTG
2402 CACGCTGTG
EcoNI 521 CCTGGTCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1202 CCTCCTCCAGG
MslI 1661 CATGGCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2222 CATCGCCATG
PaeR7I 673 CTCGAG C^TCGAG
2191 CTCGAG
PmaCI 912 CACGTG CAC^GTG
1404 CACGTG
PmlI 912 CACGTG CAC^GTG
1404 CACGTG
PshAI 518 GACCCTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1119 GACCTTCGTC
RsaI 683 GTAC GT^AC
1088 GTAC
SfiI 149 GGCCTCCAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
2417 GGCCCACGGGGCC
TfiI 1715 GATTC G^A[AT]TC
1763 GATTC
XhoI 673 CTCGAG C^TCGAG
2191 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 109 enzymes) #
#########################################
AccII AciI AcyI AluI AlwI
AlwNI ApaI AvaI AvaII BalI
BanI BanII BbeI BbvI BcnI
BfaI BglI BmyI BpmI BsaAI
BsaHI BsaI BsaJI BseRI BsgI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BsrI BssKI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI KasI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MspA1I MspI MvaI MvnI MwoI
NarI NciI NcoI NdeII NlaIII
NlaIV PflMI PleI PpuMI PstI
PvuII Sau3AI Sau96I ScrFI SfaNI
SfcI SfoI SmlI StyI TaqI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII