##################################
# NO CUT ENZYMES ( 59 enzymes) #
##################################
AatII Acc65I AcyI AgeI Alw44I
ApaLI AscI Asp718I AviII BbeI
BsaHI BsiEI BsiWI BsmBI BsrFI
BssHII BstEII BstPI Cfr10I CpoI
DraIII DrdI EagI EclXI Eco52I
EcoNI EcoO65I FseI FspI HgaI
Hin1I HincII HindII HpaI KasI
KpnI KspI MluI NaeI NarI
NgoMIV NotI NruI PacI PinAI
PmeI PvuI RsrII SacII SalI
SbfI SfiI SfoI SgrAI Sse8387I
StuI SwaI XbaI XcmI
###################################
# ONE CUT ENZYMES ( 56 enzymes) #
###################################
AccII 5770 CGCG CG^CG
AflII 194 CTTAAG C^TTAAG
AhdI 5897 GACCCTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 5663 CCTGAGG CC^T[ACGT]AGG
Aor51HI 5837 AGCGCT AGC^GCT
ApaI 5847 GGGCCC GGGCC^C
AseI 4016 ATTAAT AT^TAAT
AsnI 4016 ATTAAT AT^TAAT
AspEI 5897 GACCCTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 5400 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI 605 CACGTG CAC^GTG
BfrI 194 CTTAAG C^TTAAG
BglII 1306 AGATCT A^GATCT
BlpI 5618 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 5618 GCTCAGC GC^T[ACGT]AGC
BsaBI 1046 GATGCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 4853 GAGACC GGTCTC(1/5)
BspDI 1932 ATCGAT AT^CGAT
BspHI 3358 TCATGA T^CATGA
BspLU11I 388 ACATGT A^CATGT
BspMI 3730 ACCTGC ACCTGC(4/8)
BsrBI 2743 CCGCTC CCGCTC(-3/-3)
BssSI 2859 CACGAG CACGAG(-5/-1)
Bst1107I 4638 GTATAC GTA^TAC
BstUI 5770 CGCG CG^CG
BstZ17I 4638 GTATAC GTA^TAC
Bsu36I 5663 CCTGAGG CC^T[ACGT]AGG
CelII 5618 GCTCAGC GC^T[ACGT]AGC
ClaI 1932 ATCGAT AT^CGAT
Eam1105I 5897 GACCCTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 5837 AGCGCT AGC^GCT
Eco81I 5663 CCTGAGG CC^T[ACGT]AGG
EcoRI 877 GAATTC G^AATTC
EcoRV 600 GATATC GAT^ATC
EcoT22I 1322 ATGCAT ATGCA^T
MamI 1046 GATGCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1592 CAATTG C^AATTG
MunI 1592 CAATTG C^AATTG
MvnI 5770 CGCG CG^CG
NheI 6243 GCTAGC G^CTAGC
NsiI 1322 ATGCAT ATGCA^T
PaeR7I 5690 CTCGAG C^TCGAG
PflFI 5400 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 605 CACGTG CAC^GTG
PmlI 605 CACGTG CAC^GTG
PshAI 1729 GACCAAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 4016 ATTAAT AT^TAAT
RcaI 3358 TCATGA T^CATGA
SexAI 5401 ACCTGGT A^CC[AT]GGT
SmaI 3471 CCCGGG CCC^GGG
SnaBI 3132 TACGTA TAC^GTA
SpeI 581 ACTAGT A^CTAGT
Tth111I 5400 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 5690 CTCGAG C^TCGAG
XmaCI 3471 CCCGGG C^CCGGG
XmaI 3471 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AccI 384 GTAGAC GT^[AC][GT]AC
4638 GTATAC
AccIII 4004 TCCGGA T^CCGGA
4492 TCCGGA
AclI 1356 AACGTT AA^CGTT
2167 AACGTT
Asp700I 2423 GAAGATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4582 GAATAGCTTC
AvrII 659 CCTAGG C^CTAGG
5926 CCTAGG
BamHI 2059 GGATCC G^GATCC
5603 GGATCC
BcgI 4702 CGAGCATCATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
306 GCATCAAAATCG
BciVI 3788 GGATAC GTATCC(6/5)
476 GTATCC
BglI 425 GCCTTTCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
5260 GCCTCACAGGC
BlnI 659 CCTAGG C^CTAGG
5926 CCTAGG
BpmI 4763 CTCCAG CTGGAG(16/14)
1632 CTGGAG
BsaWI 4004 TCCGGA [AT]^CCGG[AT]
4492 TCCGGA
BseAI 4004 TCCGGA T^CCGGA
4492 TCCGGA
Bsp1407I 2872 TGTACA T^GTACA
5287 TGTACA
BspEI 4004 TCCGGA T^CCGGA
4492 TCCGGA
BsrGI 2872 TGTACA T^GTACA
5287 TGTACA
BstBI 3045 TTCGAA TT^CGAA
4026 TTCGAA
BstXI 2960 CCAGTAGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3568 CCAGAATGATGG
MroI 4004 TCCGGA T^CCGGA
4492 TCCGGA
NspV 3045 TTCGAA TT^CGAA
4026 TTCGAA
PflMI 2019 CCATACCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2536 CCAGGCCATGG
PpuMI 4420 GGGACCC [GA]G^G[AT]CC[CT]
5398 AGGACCT
Psp1406I 1356 AACGTT AA^CGTT
2167 AACGTT
PvuII 452 CAGCTG CAG^CTG
2123 CAGCTG
SapI 156 GAAGAGC GCTCTTC(1/4)
1420 GAAGAGC
SfuI 3045 TTCGAA TT^CGAA
4026 TTCGAA
SphI 447 GCATGC GCATG^C
1324 GCATGC
SspBI 2872 TGTACA T^GTACA
5287 TGTACA
SspI 1213 AATATT AAT^ATT
6077 AATATT
Van91I 2019 CCATACCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2536 CCAGGCCATGG
XmnI 2423 GAAGATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4582 GAATAGCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 112 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI ApoI AspHI AvaI
AvaII BalI BanI BanII BbsI
BbvCI BbvI BclI BcnI BfaI
BmyI BpuAI BsaAI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I BsrDI
BsrI BssKI BstAPI BstF5I BstNI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraI DraII DsaI EaeI
EarI EcoO109I EcoRII EcoT14I FauI
FbaI Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HindIII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MwoI NciI
NcoI NdeI NdeII NlaIII NlaIV
NspI PleI PstI RsaI SacI
Sau3AI Sau96I ScaI ScrFI SfaNI
SfcI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII