##################################
# NO CUT ENZYMES ( 73 enzymes) #
##################################
AatII AflII AgeI AhdI AocI
AscI AseI AsnI AspEI AspI
AvrII BamHI BbeI BcgI BfrI
BglI BlnI BsiEI BsiWI BsmBI
BsrFI BstBI BstXI Bsu36I Cfr10I
CpoI DrdI EagI Eam1105I EclXI
Eco52I Eco81I EcoT22I FseI HindIII
KasI MfeI MluI MunI NaeI
NarI NcoI NgoMIV NheI NotI
NruI NsiI NspV PacI PflFI
PinAI PmeI PpuMI PshAI PshBI
PvuI RsrII SalI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SphI Sse8387I SwaI
Tth111I XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 57 enzymes) #
###################################
Acc65I 3874 GGTACC G^GTACC
AccI 1247 GTATAC GT^[AC][GT]AC
AclI 3352 AACGTT AA^CGTT
AlwI 2254 GGATC GGATC(4/5)
Aor51HI 2458 AGCGCT AGC^GCT
ApaI 128 GGGCCC GGGCC^C
Asp718I 3874 GGTACC G^GTACC
AvaI 46 CTCGAG C^[CT]CG[GA]G
AviII 1186 TGCGCA TGC^GCA
BalI 3762 TGGCCA TGG^CCA
BanI 3874 GGTACC G^G[CT][GA]CC
BbrPI 4127 CACGTG CAC^GTG
BbsI 383 GAAGAC GAAGAC(2/6)
BbvCI 1936 CCTCAGC CCTCAGC(-5/-2)
BpmI 1453 CTCCAG CTGGAG(16/14)
BpuAI 383 GAAGAC GAAGAC(2/6)
BsaBI 3308 GATAGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 2377 CTGCAC GTGCAG(16/14)
BsoBI 46 CTCGAG C^[CT]CG[GA]G
BspDI 4108 ATCGAT AT^CGAT
BsrBI 2430 GAGCGG CCGCTC(-3/-3)
BssHII 96 GCGCGC G^CGCGC
BssSI 3649 CACGAG CACGAG(-5/-1)
Bst1107I 1247 GTATAC GTA^TAC
BstEII 199 GGTTACC G^GT[ACGT]ACC
BstPI 199 GGTTACC G^GT[ACGT]ACC
BstZ17I 1247 GTATAC GTA^TAC
ClaI 4108 ATCGAT AT^CGAT
DraIII 2233 CACGAAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 223 CCGCGG C^C[GA][CT]GG
EaeI 3762 TGGCCA [CT]^GGCC[GA]
Eco47III 2458 AGCGCT AGC^GCT
EcoNI 4203 CCTTTCTTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 199 GGTTACC G^GT[ACGT]ACC
EcoRV 3585 GATATC GAT^ATC
EcoT14I 30 CCAAGG C^C[AT][AT]GG
FspI 1186 TGCGCA TGC^GCA
HaeII 2458 AGCGCT [GA]GCGC^[CT]
KpnI 3874 GGTACC GGTAC^C
KspI 223 CCGCGG CCGC^GG
MamI 3308 GATAGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 3762 TGGCCA TGG^CCA
MscI 3762 TGGCCA TGG^CCA
NdeI 2346 CATATG CA^TATG
PaeR7I 46 CTCGAG C^TCGAG
PmaCI 4127 CACGTG CAC^GTG
PmlI 4127 CACGTG CAC^GTG
Psp1406I 3352 AACGTT AA^CGTT
PvuII 191 CAGCTG CAG^CTG
SacI 3283 GAGCTC GAGCT^C
SacII 223 CCGCGG CCGC^GG
SpeI 3692 ACTAGT A^CTAGT
SspI 2687 AATATT AAT^ATT
StuI 3157 AGGCCT AGG^CCT
StyI 30 CCAAGG C^C[AT][AT]GG
XbaI 4044 TCTAGA T^CTAGA
XhoI 46 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccII 97 CGCG CG^CG
224 CGCG
AccIII 670 TCCGGA T^CCGGA
1138 TCCGGA
Alw44I 426 GTGCAC G^TGCAC
4261 GTGCAC
ApaLI 426 GTGCAC G^TGCAC
4261 GTGCAC
Asp700I 1338 GAATCAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2928 GAATTGCTTC
BanII 128 GGGCCC G[GA]GC[CT]^C
3283 GAGCTC
BciVI 2180 GGATAC GTATCC(6/5)
2904 GGATAC
BclI 3011 TGATCA T^GATCA
3569 TGATCA
BcnI 101 CCCGG CC^[GC]GG
205 CCCGG
BsaAI 513 TACGTG [CT]AC^GT[GA]
4127 CACGTG
BsaI 843 GAGACC GGTCTC(1/5)
2952 GGTCTC
BseAI 670 TCCGGA T^CCGGA
1138 TCCGGA
BsmI 462 GAATGC GAATGC(1/-1)
934 GAATGC
BspEI 670 TCCGGA T^CCGGA
1138 TCCGGA
BspHI 2815 TCATGA T^CATGA
3572 TCATGA
BspLU11I 2556 ACATGT A^CATGT
3129 ACATGT
BstUI 97 CGCG CG^CG
224 CGCG
DraII 128 GGGCCCT [GA]G^G[ACGT]CC[CT]
489 AGGGCCT
EcoO109I 128 GGGCCCT [GA]G^G[ACGT]CC[CT]
489 AGGGCCT
EcoRI 2152 GAATTC G^AATTC
3775 GAATTC
FauI 119 GCGGG CCCGC(4/6)
2432 GCGGG
FbaI 3011 TGATCA T^GATCA
3569 TGATCA
HpaI 1685 GTTAAC GTT^AAC
4191 GTTAAC
MroI 670 TCCGGA T^CCGGA
1138 TCCGGA
MvnI 97 CGCG CG^CG
224 CGCG
NciI 101 CCCGG CC^[GC]GG
205 CCCGG
PstI 3034 CTGCAG CTGCA^G
3269 CTGCAG
RcaI 2815 TCATGA T^CATGA
3572 TCATGA
SapI 1572 GAAGAGC GCTCTTC(1/4)
1725 GCTCTTC
XmnI 1338 GAATCAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2928 GAATTGCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 98 enzymes) #
#########################################
AciI AcsI AcyI AfaI AflIII
AluI AlwNI ApoI AspHI AvaII
BbvI BfaI BglII BlpI BmyI
Bpu1102I BsaHI BsaJI BsaWI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I Bsp1407I BspMI BsrDI BsrGI
BsrI BssKI BstAPI BstF5I BstNI
BstYI Cac8I CelII CfoI Cfr13I
DdeI DpnII DraI EarI EcoRII
Fnu4HI FokI HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MwoI NdeII NlaIII
NlaIV NspI PflMI PleI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspBI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII