##################################
# NO CUT ENZYMES ( 69 enzymes) #
##################################
AatII AccI AccII AflII AgeI
AhdI Alw44I ApaI ApaLI AscI
Asp700I AspEI AviII AvrII BfrI
BlnI BsaBI BsiEI BsiWI BspLU11I
BsrBI BssHII Bst1107I BstEII BstPI
BstUI BstZ17I CpoI EagI Eam1105I
EclXI Eco52I EcoO65I EcoRV FseI
FspI KspI MamI MfeI MluI
MunI MvnI NaeI NcoI NdeI
NgoMIV NotI NruI PacI PflMI
PinAI PmeI PvuI RsrII SacII
SalI SbfI ScaI SexAI SfiI
SgrAI SnaBI SpeI SphI Sse8387I
StuI Van91I XcmI XmnI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
Acc65I 1529 GGTACC G^GTACC
AclI 1694 AACGTT AA^CGTT
AflIII 3459 ACACGT A^C[GA][CT]GT
Aor51HI 4628 AGCGCT AGC^GCT
AseI 949 ATTAAT AT^TAAT
AsnI 949 ATTAAT AT^TAAT
Asp718I 1529 GGTACC G^GTACC
AspI 4336 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 1396 GGCGCC GGCGC^C
BbrPI 4247 CACGTG CAC^GTG
BciVI 1473 GTATCC GTATCC(6/5)
BclI 2409 TGATCA T^GATCA
BglI 3854 GCCGGGACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 3506 AGATCT A^GATCT
BlpI 1853 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1853 GCTCAGC GC^T[ACGT]AGC
BsaAI 4247 CACGTG [CT]AC^GT[GA]
BspDI 3104 ATCGAT AT^CGAT
BspHI 796 TCATGA T^CATGA
BsrFI 12 GCCGGT [GA]^CCGG[CT]
BstBI 663 TTCGAA TT^CGAA
BstXI 2946 CCATCATGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 1853 GCTCAGC GC^T[ACGT]AGC
Cfr10I 12 GCCGGT [GA]^CCGG[CT]
ClaI 3104 ATCGAT AT^CGAT
Eco47III 4628 AGCGCT AGC^GCT
EcoT22I 454 ATGCAT ATGCA^T
FbaI 2409 TGATCA T^GATCA
HindIII 3491 AAGCTT A^AGCTT
HpaI 4880 GTTAAC GTT^AAC
KasI 1396 GGCGCC G^GCGCC
KpnI 1529 GGTACC GGTAC^C
NarI 1396 GGCGCC GG^CGCC
NheI 3832 GCTAGC G^CTAGC
NsiI 454 ATGCAT ATGCA^T
NspV 663 TTCGAA TT^CGAA
PaeR7I 2873 CTCGAG C^TCGAG
PflFI 4336 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 4247 CACGTG CAC^GTG
PmlI 4247 CACGTG CAC^GTG
PshAI 1784 GACCAGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 949 ATTAAT AT^TAAT
Psp1406I 1694 AACGTT AA^CGTT
RcaI 796 TCATGA T^CATGA
SfoI 1396 GGCGCC GGC^GCC
SfuI 663 TTCGAA TT^CGAA
SwaI 2079 ATTTAAAT ATTT^AAAT
Tth111I 4336 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 2873 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AccIII 1812 TCCGGA T^CCGGA
3009 TCCGGA
BalI 3931 TGGCCA TGG^CCA
3994 TGGCCA
BamHI 142 GGATCC G^GATCC
971 GGATCC
BcgI 3096 GCACTCTGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
4129 GCATCGGAGTCG
BsaI 2397 GGTCTC GGTCTC(1/5)
4446 GGTCTC
BsaWI 1812 TCCGGA [AT]^CCGG[AT]
3009 TCCGGA
BseAI 1812 TCCGGA T^CCGGA
3009 TCCGGA
BsmBI 2736 CGTCTC CGTCTC(1/5)
3310 GAGACG
BspEI 1812 TCCGGA T^CCGGA
3009 TCCGGA
DsaI 4110 CCGTGG C^C[GA][CT]GG
4331 CCACGG
EcoRI 4093 GAATTC G^AATTC
4888 GAATTC
MluNI 3931 TGGCCA TGG^CCA
3994 TGGCCA
MroI 1812 TCCGGA T^CCGGA
3009 TCCGGA
MscI 3931 TGGCCA TGG^CCA
3994 TGGCCA
NspI 2791 ACATGC [GA]CATG^[CT]
3841 ACATGC
XbaI 2401 TCTAGA T^CTAGA
3337 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 124 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
AlwI AlwNI AocI ApoI AspHI
AvaI AvaII BanI BanII BbsI
BbvCI BbvI BcnI BfaI BmyI
BpmI BpuAI BsaHI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I Bsp1407I
BspMI BsrDI BsrGI BsrI BssKI
BssSI BstAPI BstF5I BstNI BstYI
Bsu36I Cac8I CfoI Cfr13I DdeI
DpnII DraI DraII DraIII DrdI
EaeI EarI Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV PleI PpuMI PstI PvuII
RsaI SacI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SmaI SmlI
SspBI SspI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII XmaCI XmaI