HUGE

Gene/Protein Characteristic Table for KIAA0057

Link to : GTOP \| SWISS-PROT/TrEMBL \| GeneCards\| RefDIC
Features : DNA sequence \| Protein sequence \| Expression \| Mapping

Product ID :	ORK00388
Accession No. :	D31762
Description :	Translocation-associated membrane protein 2.
HUGO Gene Name :	translocation associated membrane protein 2 (TRAM2)
Clone Name :	ha00909 [Vector Info]
Flexi ORF Clone :	pF1KSDA0057
Source :	Myeloblast cell line (KG-1)

Features of the cloned DNA sequence	Description

Length: 6974 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis) for :
cloned DNA seq.
Warning for N-terminal truncation:
NO

Warning for coding interruption:
NO

Integrity of 3' end

Length of 3'UTR 5786 bp

Genome contig ID gi89161210r_52370166
PolyA signal sequence
(AATAAA,-18)
+----*----+----*----+----*----+----
ATATTTTCTGAAATAACAATAAAAGGCAGAAAATT
Flanking genome sequence
(100000 - 99951)
----+----*----+----*----+----*----+----*----+----*
ATCAGAATTGTCTCATAATTATTTCCTTGACGTGCTTTTATACAGTGATG

Ensembl ContigView (Add our DAS server as a DAS source)

Chr f/r start end exon identity class

ContigView(URL based/DAS) 6 r 52470166 52549747 11 99.2 Perfect prediction

Features of the protein sequence	Description

Length: 384 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	Exp	ID%	Protein	Source
Q15035	1.4e-166	100.0	Translocation-a...	Homo sapiens
XP_518539	1.7e-166	99.7	translocation-a...	Pan troglodytes
XP_001107590	1.9e-165	99.2	translocation-a...	Macaca mulatta
XP_001499056	6.7e-164	97.8	similar to Tran...	Equus caballus
XP_001928862	1.7e-159	95.4	similar to Tran...	Sus scrofa

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against other HUGE database (FASTA output)
No significant homologues
Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method interpro_ID From To Entry Definition

HMMPfam IPR013599 59 125 PF08390 TRAM1-like protein

IPR005547 146 341 PF03798 Longevity-assurance protein (LAG1)

HMMSmart IPR006634 126 335 SM00724 TRAM

ProfileScan IPR006634 126 335 PS50922 TRAM

Search method	interpro_ID	From	To	Entry	Definition
HMMPfam	IPR013599	59	125	PF08390	TRAM1-like protein
IPR005547	146	341	PF03798	Longevity-assurance protein (LAG1)
HMMSmart	IPR006634	126	335	SM00724	TRAM
ProfileScan	IPR006634	126	335	PS50922	TRAM

Prediction of transmembrane (TM) segments

Method No. N terminal transmembrane region C terminal type length

SOSUI2 1 43 LVLCVLIGLMFEVTAKTAFLFIL 65 PRIMARY 23

2 87 KDLVTILFYIFITIILHAVVQEY 109 PRIMARY 23

3 132 GQLVVFHFTSVIWCFYVVVTEGY 154 SECONDARY 23

4 170 LPFQVKFFYLCQLAYWLHALPEL 192 SECONDARY 23

5 209 ICLYLVHIAGAYLLNLSRLGLIL 231 PRIMARY 23

6 262 WAAVFGVTRLFILTLAVLAIGFG 284 PRIMARY 23

7 300 FNTLFCRLCVLLLVCAAQAWLMW 322 PRIMARY 23

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	43	LVLCVLIGLMFEVTAKTAFLFIL	65	PRIMARY	23
2	87	KDLVTILFYIFITIILHAVVQEY	109	PRIMARY	23
3	132	GQLVVFHFTSVIWCFYVVVTEGY	154	SECONDARY	23
4	170	LPFQVKFFYLCQLAYWLHALPEL	192	SECONDARY	23
5	209	ICLYLVHIAGAYLLNLSRLGLIL	231	PRIMARY	23
6	262	WAAVFGVTRLFILTLAVLAIGFG	284	PRIMARY	23
7	300	FNTLFCRLCVLLLVCAAQAWLMW	322	PRIMARY	23

Expression profile	Description

: Northern blot Description

Northern blot	Description

RH mapping information	Description

Chromosome No.

: 6

Experimental conditions

Panel name	: Stanford G3
Primer_f	: CAGAAGGATGGTGTGAACTCG
Primer_r	: CTGCTGTTTGGTTTGGTTTTG
PCR product length	: 273 bp
PCR conditions	: 95 °C15 sec62 °C60 sec30 cycles

How to obtain KIAA clone(s) Back to the HUGE Protein Database homepage

	cloned DNA seq.
Warning for N-terminal truncation:	NO
Warning for coding interruption:	NO

Length of 3'UTR	5786 bp
Genome contig ID	gi89161210r_52370166
PolyA signal sequence (AATAAA,-18)	+--------+--------+----*----+---- ATATTTTCTGAAATAACAATAAAAGGCAGAAAATT
Flanking genome sequence (100000 - 99951)	----+--------+--------+--------+--------+----* ATCAGAATTGTCTCATAATTATTTCCTTGACGTGCTTTTATACAGTGATG

	Chr	f/r	start	end	exon	identity	class
ContigView(URL based/DAS)	6	r	52470166	52549747	11	99.2	Perfect prediction

Gene/Protein Characteristic Table for KIAA0057

Features of the cloned DNA sequence

Features of the protein sequence

Expression profile

RH mapping information