##################################
# NO CUT ENZYMES ( 51 enzymes) #
##################################
AclI AgeI AhdI AscI AspEI
AvaI BamHI BbeI BbvCI BcgI
BciVI BsaBI BsiWI BsoBI BspDI
ClaI CpoI DrdI Eam1105I FseI
HpaI KasI KspI MamI MluI
NarI NotI NruI PacI PaeR7I
PinAI PmeI Psp1406I PvuI RsrII
SacII SalI SbfI ScaI SfiI
SfoI SgrAI SmaI SnaBI SpeI
Sse8387I SwaI XbaI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 34 enzymes) #
###################################
Acc65I 1237 GGTACC G^GTACC
AccIII 2596 TCCGGA T^CCGGA
Aor51HI 2684 AGCGCT AGC^GCT
Asp718I 1237 GGTACC G^GTACC
AviII 5193 TGCGCA TGC^GCA
BclI 6908 TGATCA T^GATCA
BglI 7259 GCCCTGCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaWI 2596 TCCGGA [AT]^CCGG[AT]
BseAI 2596 TCCGGA T^CCGGA
BsiEI 1382 CGGCCG CG[GA][CT]^CG
BsmI 5186 GAATGC GAATGC(1/-1)
BspEI 2596 TCCGGA T^CCGGA
BspHI 3776 TCATGA T^CATGA
BssHII 7218 GCGCGC G^CGCGC
Bst1107I 2523 GTATAC GTA^TAC
BstZ17I 2523 GTATAC GTA^TAC
DraIII 3533 CACCGAGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 1382 CGGCCG C^GGCCG
EclXI 1382 CGGCCG C^GGCCG
Eco47III 2684 AGCGCT AGC^GCT
Eco52I 1382 CGGCCG C^GGCCG
FbaI 6908 TGATCA T^GATCA
FspI 5193 TGCGCA TGC^GCA
HgaI 2224 GACGC GACGC(5/10)
HincII 541 GTCAAC GT[CT]^[GA]AC
HindII 541 GTCAAC GT[CT]^[GA]AC
KpnI 1237 GGTACC GGTAC^C
MroI 2596 TCCGGA T^CCGGA
NheI 5588 GCTAGC G^CTAGC
PshAI 4738 GACATGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 4324 CTGCAG CTGCA^G
RcaI 3776 TCATGA T^CATGA
SspI 3973 AATATT AAT^ATT
StuI 1760 AGGCCT AGG^CCT
###################################
# TWO CUT ENZYMES ( 37 enzymes) #
###################################
AatII 1717 GACGTC GACGT^C
5891 GACGTC
AccII 7219 CGCG CG^CG
7272 CGCG
AcyI 1717 GACGTC G[GA]^CG[CT]C
5891 GACGTC
AflII 3869 CTTAAG C^TTAAG
5435 CTTAAG
ApaI 16 GGGCCC GGGCC^C
5087 GGGCCC
BbrPI 3752 CACGTG CAC^GTG
4058 CACGTG
BbsI 944 GAAGAC GAAGAC(2/6)
4735 GAAGAC
BfrI 3869 CTTAAG C^TTAAG
5435 CTTAAG
BglII 1127 AGATCT A^GATCT
1677 AGATCT
BlpI 2367 GCTCAGC GC^T[ACGT]AGC
5213 GCTCAGC
Bpu1102I 2367 GCTCAGC GC^T[ACGT]AGC
5213 GCTCAGC
BpuAI 944 GAAGAC GAAGAC(2/6)
4735 GAAGAC
BsaAI 3752 CACGTG [CT]AC^GT[GA]
4058 CACGTG
BsaHI 1717 GACGTC G[GA]^CG[CT]C
5891 GACGTC
BsaI 505 GAGACC GGTCTC(1/5)
4293 GAGACC
Bsp1407I 1303 TGTACA T^GTACA
6983 TGTACA
BsrGI 1303 TGTACA T^GTACA
6983 TGTACA
BstBI 3356 TTCGAA TT^CGAA
3724 TTCGAA
BstUI 7219 CGCG CG^CG
7272 CGCG
CelII 2367 GCTCAGC GC^T[ACGT]AGC
5213 GCTCAGC
EcoNI 2117 CCTCAGAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3026 CCTAGGACAGG
EcoRI 3408 GAATTC G^AATTC
7161 GAATTC
Hin1I 1717 GACGTC G[GA]^CG[CT]C
5891 GACGTC
MfeI 461 CAATTG C^AATTG
596 CAATTG
MunI 461 CAATTG C^AATTG
596 CAATTG
MvnI 7219 CGCG CG^CG
7272 CGCG
NaeI 603 GCCGGC GCC^GGC
7264 GCCGGC
NgoMIV 603 GCCGGC G^CCGGC
7264 GCCGGC
NspV 3356 TTCGAA TT^CGAA
3724 TTCGAA
PmaCI 3752 CACGTG CAC^GTG
4058 CACGTG
PmlI 3752 CACGTG CAC^GTG
4058 CACGTG
PvuII 842 CAGCTG CAG^CTG
5120 CAGCTG
SacI 129 GAGCTC GAGCT^C
3046 GAGCTC
SapI 1734 GAAGAGC GCTCTTC(1/4)
5564 GAAGAGC
SexAI 1025 ACCAGGT A^CC[AT]GGT
5146 ACCTGGT
SfuI 3356 TTCGAA TT^CGAA
3724 TTCGAA
SspBI 1303 TGTACA T^GTACA
6983 TGTACA
#########################################
# 3 OR MORE CUT ENZYMES ( 136 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI Alw44I AlwI AlwNI AocI
ApaLI ApoI AseI AsnI Asp700I
AspHI AspI AvaII AvrII BalI
BanI BanII BbvI BcnI BfaI
BlnI BmyI BpmI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI Bsp1286I BspLU11I BspMI
BsrBI BsrDI BsrFI BsrI BssKI
BssSI BstAPI BstEII BstF5I BstNI
BstPI BstXI BstYI Bsu36I Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraI DraII DsaI EaeI EarI
Eco81I EcoO109I EcoO65I EcoRII EcoRV
EcoT14I EcoT22I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HindIII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NciI NcoI NdeI NdeII
NlaIII NlaIV NsiI NspI PflFI
PflMI PleI PpuMI PshBI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SphI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
Tth111I TthHB8I Van91I XcmI XhoII
XmnI