##################################
# NO CUT ENZYMES ( 94 enzymes) #
##################################
AatII AccIII AclI AcyI AgeI
Alw44I ApaLI AscI AseI AsnI
AviII BbeI BbrPI BbvCI BcgI
BclI BglI BglII BlpI Bpu1102I
BsaHI BsaWI BseAI BsgI BsiWI
BsmBI BspDI BspEI BspHI BsrFI
BssHII BssSI Bst1107I BstAPI BstEII
BstPI BstZ17I CelII Cfr10I ClaI
CpoI DrdI EagI EclXI Eco52I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI Hin1I HindIII HpaI
KasI KspI MfeI MluI MroI
MspA1I MunI NaeI NarI NgoMIV
NheI NotI NruI NsiI PacI
PinAI PmaCI PmeI PmlI PshBI
Psp1406I PvuI PvuII RcaI RsrII
SacI SacII SapI SexAI SfiI
SfoI SgrAI SnaBI SpeI SphI
SspI SwaI TfiI XbaI
###################################
# ONE CUT ENZYMES ( 56 enzymes) #
###################################
Acc65I 2498 GGTACC G^GTACC
AccI 735 GTCGAC GT^[AC][GT]AC
AflII 2135 CTTAAG C^TTAAG
AflIII 1066 ACATGT A^C[GA][CT]GT
AhdI 1282 GACCTAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 2077 CAGACACTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 31 AGCGCT AGC^GCT
ApaI 1914 GGGCCC GGGCC^C
Asp718I 2498 GGTACC G^GTACC
AspEI 1282 GACCTAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1073 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 2185 CCTAGG C^CTAGG
BamHI 2333 GGATCC G^GATCC
BfrI 2135 CTTAAG C^TTAAG
BlnI 2185 CCTAGG C^CTAGG
BsaAI 621 TACGTG [CT]AC^GT[GA]
BsaBI 1001 GATCCTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 637 GAGACC GGTCTC(1/5)
BsiEI 158 CGACCG CG[GA][CT]^CG
BsmI 728 GAATGC GAATGC(1/-1)
BspLU11I 1066 ACATGT A^CATGT
BsrBI 2170 CCGCTC CCGCTC(-3/-3)
BstBI 1247 TTCGAA TT^CGAA
BstYI 2333 GGATCC [GA]^GATC[CT]
DraIII 2263 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 1282 GACCTAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 765 GAAGAG CTCTTC(1/4)
Eco47III 31 AGCGCT AGC^GCT
EcoNI 2471 CCTAGCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HaeII 31 AGCGCT [GA]GCGC^[CT]
HgaI 881 GACGC GACGC(5/10)
HincII 735 GTCGAC GT[CT]^[GA]AC
HindII 735 GTCGAC GT[CT]^[GA]AC
KpnI 2498 GGTACC GGTAC^C
Ksp632I 765 GAAGAG CTCTTC(1/4)
MamI 1001 GATCCTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 2333 GGATCC [GA]^GATC[CT]
NdeI 1568 CATATG CA^TATG
NspI 1066 ACATGT [GA]CATG^[CT]
NspV 1247 TTCGAA TT^CGAA
PaeR7I 634 CTCGAG C^TCGAG
PflFI 1073 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 713 CCACATTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 377 GACCGAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SalI 735 GTCGAC G^TCGAC
SbfI 386 CCTGCAGG CCTGCA^GG
ScaI 499 AGTACT AGT^ACT
SfuI 1247 TTCGAA TT^CGAA
SmaI 68 CCCGGG CCC^GGG
Sse8387I 386 CCTGCAGG CCTGCA^GG
Tth111I 1073 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 713 CCACATTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 634 CTCGAG C^TCGAG
XhoII 2333 GGATCC [GA]^GATC[CT]
XmaCI 68 CCCGGG C^CCGGG
XmaI 68 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AcsI 1589 GAATTT [GA]^AATT[CT]
2258 AAATTC
AocI 1940 CCTCAGG CC^T[ACGT]AGG
2482 CCTAAGG
ApoI 1589 GAATTT [GA]^AATT[CT]
2258 AAATTC
AspHI 420 GAGCAC G[AT]GC[AT]^C
1015 GTGCTC
BanI 941 GGTGCC G^G[CT][GA]CC
2498 GGTACC
BciVI 893 GGATAC GTATCC(6/5)
1651 GGATAC
BsiHKAI 420 GAGCAC G[AT]GC[AT]^C
1015 GTGCTC
BsmAI 637 GAGAC GTCTC(1/5)
1761 GTCTC
Bsp1407I 1036 TGTACA T^GTACA
1207 TGTACA
BsrDI 830 CATTGC GCAATG(2/0)
1783 GCAATG
BsrGI 1036 TGTACA T^GTACA
1207 TGTACA
BstXI 1376 CCAGATAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1407 CCATGAGGCTGG
Bsu36I 1940 CCTCAGG CC^T[ACGT]AGG
2482 CCTAAGG
DraI 588 TTTAAA TTT^AAA
1532 TTTAAA
Eco81I 1940 CCTCAGG CC^T[ACGT]AGG
2482 CCTAAGG
HinfI 1667 GAGTC G^A[ACGT]TC
1947 GACTC
MaeII 622 ACGT A^CGT
1924 ACGT
PleI 1947 GACTC GAGTC(4/5)
1667 GAGTC
PstI 387 CTGCAG CTGCA^G
781 CTGCAG
SspBI 1036 TGTACA T^GTACA
1207 TGTACA
XcmI 713 CCACATTCTGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1513 CCAAACCCAGGTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 87 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
Asp700I AvaI AvaII BalI BanII
BbsI BbvI BcnI BfaI BmyI
BpmI BpuAI BsaJI BseRI BsiYI
BslI BsmFI BsoBI Bsp1286I BspMI
BsrI BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HpaII HphI
ItaI MaeI MaeIII MboI MboII
MluNI MnlI MscI MseI MslI
MspI MvaI MvnI MwoI NciI
NcoI NdeII NlaIII NlaIV PpuMI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StuI StyI TaqI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XmnI