##################################
# NO CUT ENZYMES ( 87 enzymes) #
##################################
AatII AccII AclI AgeI AhdI
Aor51HI ApaI AscI AseI AsnI
Asp700I AspEI AspI AviII BamHI
BbrPI BclI BglI BlpI Bpu1102I
BsiWI BsmBI BsmI Bsp1407I BspDI
BsrGI BssHII BssSI Bst1107I BstBI
BstUI BstZ17I CelII ClaI CpoI
DraIII DrdI Eam1105I Eco47III EcoRI
EcoRV EcoT22I FbaI FseI FspI
HpaI KspI MfeI MluI MunI
MvnI NaeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI PvuII
RsrII SacII SalI SbfI ScaI
SexAI SfiI SfuI SgrAI SnaBI
SpeI Sse8387I SspBI SwaI Tth111I
XhoI XmnI
###################################
# ONE CUT ENZYMES ( 59 enzymes) #
###################################
Acc65I 840 GGTACC G^GTACC
AccI 2076 GTAGAC GT^[AC][GT]AC
AccIII 193 TCCGGA T^CCGGA
AcyI 1596 GGCGCC G[GA]^CG[CT]C
AflII 1100 CTTAAG C^TTAAG
Alw44I 1095 GTGCAC G^TGCAC
AlwNI 1206 CAGGTTCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 184 CCTCAGG CC^T[ACGT]AGG
ApaLI 1095 GTGCAC G^TGCAC
Asp718I 840 GGTACC G^GTACC
AvrII 3058 CCTAGG C^CTAGG
BbeI 1596 GGCGCC GGCGC^C
BcgI 590 GCAGGGATGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 1100 CTTAAG C^TTAAG
BglII 1008 AGATCT A^GATCT
BlnI 3058 CCTAGG C^CTAGG
BsaAI 1444 TACGTG [CT]AC^GT[GA]
BsaBI 123 GATTCCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1596 GGCGCC G[GA]^CG[CT]C
BsaWI 193 TCCGGA [AT]^CCGG[AT]
BseAI 193 TCCGGA T^CCGGA
BsiEI 2621 CGGCCG CG[GA][CT]^CG
BspEI 193 TCCGGA T^CCGGA
BspHI 635 TCATGA T^CATGA
BspLU11I 2534 ACATGT A^CATGT
BsrBI 1155 GAGCGG CCGCTC(-3/-3)
BsrFI 2619 ACCGGC [GA]^CCGG[CT]
BstAPI 1298 GCAGATAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 2347 GGTGACC G^GT[ACGT]ACC
BstPI 2347 GGTGACC G^GT[ACGT]ACC
Bsu36I 184 CCTCAGG CC^T[ACGT]AGG
Cfr10I 2619 ACCGGC [GA]^CCGG[CT]
DraI 22 TTTAAA TTT^AAA
EagI 2621 CGGCCG C^GGCCG
EclXI 2621 CGGCCG C^GGCCG
Eco52I 2621 CGGCCG C^GGCCG
Eco81I 184 CCTCAGG CC^T[ACGT]AGG
EcoNI 1070 CCTGGGCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 2347 GGTGACC G^GT[ACGT]ACC
HaeII 1596 GGCGCC [GA]GCGC^[CT]
HgaI 2909 GACGC GACGC(5/10)
Hin1I 1596 GGCGCC G[GA]^CG[CT]C
HincII 2511 GTTGAC GT[CT]^[GA]AC
HindII 2511 GTTGAC GT[CT]^[GA]AC
KasI 1596 GGCGCC G^GCGCC
KpnI 840 GGTACC GGTAC^C
MamI 123 GATTCCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 193 TCCGGA T^CCGGA
MspA1I 1384 CAGCGG C[AC]G^C[GT]G
NarI 1596 GGCGCC GG^CGCC
NdeI 2293 CATATG CA^TATG
PstI 1296 CTGCAG CTGCA^G
RcaI 635 TCATGA T^CATGA
SapI 498 GAAGAGC GCTCTTC(1/4)
SfoI 1596 GGCGCC GGC^GCC
SmaI 434 CCCGGG CCC^GGG
XbaI 225 TCTAGA T^CTAGA
XmaCI 434 CCCGGG C^CCGGG
XmaI 434 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AcsI 25 AAATTC [GA]^AATT[CT]
1994 AAATTC
AfaI 841 GTAC GT^AC
1389 GTAC
AflIII 1032 ACGTGT A^C[GA][CT]GT
2534 ACATGT
AlwI 2450 GATCC GGATC(4/5)
698 GGATC
ApoI 25 AAATTC [GA]^AATT[CT]
1994 AAATTC
AvaI 434 CCCGGG C^[CT]CG[GA]G
757 CCCGAG
BalI 1438 TGGCCA TGG^CCA
2402 TGGCCA
BanII 781 GAGCTC G[GA]GC[CT]^C
2676 GAGCTC
BbsI 2038 GAAGAC GAAGAC(2/6)
409 GTCTTC
BbvCI 913 CCTCAGC CCTCAGC(-5/-2)
1567 CCTCAGC
BciVI 438 GGATAC GTATCC(6/5)
1800 GGATAC
BpuAI 2038 GAAGAC GAAGAC(2/6)
409 GTCTTC
BsaI 1352 GGTCTC GGTCTC(1/5)
1667 GGTCTC
BsoBI 434 CCCGGG C^[CT]CG[GA]G
757 CCCGAG
BstYI 698 GGATCT [GA]^GATC[CT]
1008 AGATCT
HindIII 209 AAGCTT A^AGCTT
2460 AAGCTT
MflI 698 GGATCT [GA]^GATC[CT]
1008 AGATCT
MluNI 1438 TGGCCA TGG^CCA
2402 TGGCCA
MscI 1438 TGGCCA TGG^CCA
2402 TGGCCA
RsaI 841 GTAC GT^AC
1389 GTAC
SacI 781 GAGCTC GAGCT^C
2676 GAGCTC
SphI 503 GCATGC GCATG^C
1394 GCATGC
SspI 1777 AATATT AAT^ATT
3147 AATATT
StuI 1564 AGGCCT AGG^CCT
3026 AGGCCT
TaqI 708 TCGA T^CGA
737 TCGA
TthHB8I 708 TCGA T^CGA
737 TCGA
XcmI 2113 CCAAGCTCAGGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2306 CCAAAGTGGATGTGG
XhoII 698 GGATCT [GA]^GATC[CT]
1008 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 84 enzymes) #
#########################################
AciI AluI AspHI AvaII BanI
BbvI BcnI BfaI BmyI BpmI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BspMI
BsrDI BsrI BssKI BstF5I BstNI
BstXI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MnlI MseI MslI MspI MvaI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PflMI PleI PpuMI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI Van91I