##################################
# NO CUT ENZYMES ( 99 enzymes) #
##################################
Acc65I AccI AclI AflII AflIII
AgeI AhdI Alw44I AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspEI AviII AvrII BamHI
BanI BbeI BbsI BclI BfrI
BglII BlnI BpuAI BsaI BsiWI
Bsp1407I BspDI BspLU11I BsrBI BsrGI
BssHII Bst1107I BstBI BstZ17I Bsu36I
ClaI CpoI DraI DraIII DrdI
Eam1105I Eco47III Eco81I EcoRI EcoRV
EcoT22I FbaI FseI FspI HaeII
HpaI KasI KpnI KspI MfeI
MluI MunI NarI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PinAI PmeI PshAI PshBI
Psp1406I PvuI PvuII RsrII SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SnaBI SpeI
Sse8387I SspBI SspI StuI SwaI
TfiI XbaI XcmI XhoI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AatII 75 GACGTC GACGT^C
AccII 1095 CGCG CG^CG
AcsI 1672 GAATTT [GA]^AATT[CT]
AcyI 75 GACGTC G[GA]^CG[CT]C
ApoI 1672 GAATTT [GA]^AATT[CT]
AspHI 1108 GAGCTC G[AT]GC[AT]^C
AspI 856 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 1496 CCCGGG C^[CT]CG[GA]G
BbrPI 298 CACGTG CAC^GTG
BbvCI 430 GCTGAGG CCTCAGC(-5/-2)
BcgI 471 GCAAGCTGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BpmI 649 CTGGAG CTGGAG(16/14)
BsaHI 75 GACGTC G[GA]^CG[CT]C
BsiEI 199 CGGCCG CG[GA][CT]^CG
BsiHKAI 1108 GAGCTC G[AT]GC[AT]^C
BsmBI 1434 GAGACG CGTCTC(1/5)
BsmI 394 GCATTC GAATGC(1/-1)
BsoBI 1496 CCCGGG C^[CT]CG[GA]G
BstAPI 1172 GCATGCGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 1095 CGCG CG^CG
BstXI 1970 CCAAGGGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
EagI 199 CGGCCG C^GGCCG
EarI 828 GAAGAG CTCTTC(1/4)
EclXI 199 CGGCCG C^GGCCG
Eco52I 199 CGGCCG C^GGCCG
EcoNI 1906 CCTTGGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
Hin1I 75 GACGTC G[GA]^CG[CT]C
HincII 613 GTCAAC GT[CT]^[GA]AC
HindII 613 GTCAAC GT[CT]^[GA]AC
HindIII 409 AAGCTT A^AGCTT
Ksp632I 828 GAAGAG CTCTTC(1/4)
MseI 2020 TTAA T^TAA
MvnI 1095 CGCG CG^CG
NaeI 1412 GCCGGC GCC^GGC
NgoMIV 1412 GCCGGC G^CCGGC
PflFI 856 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 298 CACGTG CAC^GTG
PmlI 298 CACGTG CAC^GTG
PpuMI 554 AGGTCCC [GA]G^G[AT]CC[CT]
PstI 1117 CTGCAG CTGCA^G
SacI 1108 GAGCTC GAGCT^C
SgrAI 1411 CGCCGGCG C[GA]^CCGG[CT]G
SmaI 1496 CCCGGG CCC^GGG
Tru9I 2020 TTAA T^TAA
Tth111I 856 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 1496 CCCGGG C^CCGGG
XmaI 1496 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccIII 6 TCCGGA T^CCGGA
190 TCCGGA
AlwNI 337 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG
1012 CAGACGCTG
Asp700I 408 GAAGCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
990 GAAGGAGTTC
BalI 1134 TGGCCA TGG^CCA
1763 TGGCCA
BciVI 694 GGATAC GTATCC(6/5)
1777 GTATCC
BfaI 1316 CTAG C^TAG
1711 CTAG
BsaAI 280 TACGTG [CT]AC^GT[GA]
298 CACGTG
BsaBI 1335 GATGAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1621 GATGAGGATC
BsaWI 6 TCCGGA [AT]^CCGG[AT]
190 TCCGGA
BseAI 6 TCCGGA T^CCGGA
190 TCCGGA
BseRI 1194 CTCCTC GAGGAG(10/8)
444 GAGGAG
BspEI 6 TCCGGA T^CCGGA
190 TCCGGA
BspHI 1280 TCATGA T^CATGA
1331 TCATGA
BspMI 887 ACCTGC ACCTGC(4/8)
552 GCAGGT
BsrDI 491 CATTGC GCAATG(2/0)
1205 CATTGC
BssSI 352 CACGAG CACGAG(-5/-1)
1221 CACGAG
DraII 554 AGGTCCC [GA]G^G[ACGT]CC[CT]
1769 GGGGCCT
EcoO109I 554 AGGTCCC [GA]G^G[ACGT]CC[CT]
1769 GGGGCCT
MaeI 1316 CTAG C^TAG
1711 CTAG
MamI 1335 GATGAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1621 GATGAGGATC
MluNI 1134 TGGCCA TGG^CCA
1763 TGGCCA
MroI 6 TCCGGA T^CCGGA
190 TCCGGA
MscI 1134 TGGCCA TGG^CCA
1763 TGGCCA
RcaI 1280 TCATGA T^CATGA
1331 TCATGA
SmlI 1477 CTTGAG C^T[CT][GA]AG
1518 CTCAAG
SphI 46 GCATGC GCATG^C
1172 GCATGC
XmnI 408 GAAGCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
990 GAAGGAGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 85 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaII
BanII BbvI BcnI BglI BlpI
BmyI Bpu1102I BsaJI BsgI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrFI
BsrI BssKI BstEII BstF5I BstNI
BstPI BstYI Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DsaI
EaeI EcoO65I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI MaeII MaeIII MboI MboII
MflI MnlI MslI MspA1I MspI
MvaI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PflMI PleI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI StyI TaqI TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XhoII