##################################
# NO CUT ENZYMES ( 78 enzymes) #
##################################
Acc65I AccII AccIII AclI Aor51HI
AscI AseI AsnI Asp718I AviII
AvrII BbeI BcgI BclI BglI
BlnI BlpI Bpu1102I BseAI BsiWI
BsmBI BspEI BspHI BssHII BssSI
Bst1107I BstAPI BstBI BstUI BstZ17I
CelII CpoI DraI EagI EclXI
Eco47III Eco52I EcoNI FbaI FseI
FspI HaeII KasI KpnI KspI
MfeI MluI MroI MslI MunI
MvnI NarI NotI NruI NspV
PacI PmeI PshAI PshBI Psp1406I
PvuI PvuII RcaI RsrII SacI
SacII SalI SbfI SexAI SfiI
SfoI SfuI SgrAI SnaBI Sse8387I
SspI SwaI XbaI
###################################
# ONE CUT ENZYMES ( 58 enzymes) #
###################################
AatII 3949 GACGTC GACGT^C
AflII 554 CTTAAG C^TTAAG
AhdI 4037 GACCCGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2459 GTGCAC G^TGCAC
ApaI 1756 GGGCCC GGGCC^C
ApaLI 2459 GTGCAC G^TGCAC
AspEI 4037 GACCCGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 3386 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 1136 TGGCCA TGG^CCA
BanI 732 GGCACC G^G[CT][GA]CC
BbrPI 1166 CACGTG CAC^GTG
BfrI 554 CTTAAG C^TTAAG
BglII 2936 AGATCT A^GATCT
BsaAI 1166 CACGTG [CT]AC^GT[GA]
BsaBI 894 GATGGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1980 GGTCTC GGTCTC(1/5)
BsiEI 3224 CGACCG CG[GA][CT]^CG
BsmI 3939 GAATGC GAATGC(1/-1)
Bsp1407I 245 TGTACA T^GTACA
BspDI 216 ATCGAT AT^CGAT
BspLU11I 2455 ACATGT A^CATGT
BsrBI 1752 GAGCGG CCGCTC(-3/-3)
BsrDI 2552 CATTGC GCAATG(2/0)
BsrGI 245 TGTACA T^GTACA
BstEII 672 GGTGACC G^GT[ACGT]ACC
BstPI 672 GGTGACC G^GT[ACGT]ACC
CfoI 3655 GCGC GCG^C
ClaI 216 ATCGAT AT^CGAT
DrdI 675 GACCTGATAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 4037 GACCCGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 672 GGTGACC G^GT[ACGT]ACC
EcoRI 694 GAATTC G^AATTC
EcoRV 3480 GATATC GAT^ATC
FauI 1754 GCGGG CCCGC(4/6)
HhaI 3655 GCGC GCG^C
HinP1I 3655 GCGC G^CGC
HpaI 1935 GTTAAC GTT^AAC
MamI 894 GATGGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 1136 TGGCCA TGG^CCA
MscI 1136 TGGCCA TGG^CCA
NaeI 1027 GCCGGC GCC^GGC
NdeI 2071 CATATG CA^TATG
NgoMIV 1027 GCCGGC G^CCGGC
PaeR7I 1837 CTCGAG C^TCGAG
PflFI 3386 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 2934 CCAGATCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1166 CACGTG CAC^GTG
PmlI 1166 CACGTG CAC^GTG
PpuMI 2773 AGGACCC [GA]G^G[AT]CC[CT]
PstI 1817 CTGCAG CTGCA^G
SapI 605 GAAGAGC GCTCTTC(1/4)
ScaI 2641 AGTACT AGT^ACT
SpeI 3324 ACTAGT A^CTAGT
SspBI 245 TGTACA T^GTACA
StuI 3154 AGGCCT AGG^CCT
Tth111I 3386 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 2934 CCAGATCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 1837 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AcyI 1927 GGCGTC G[GA]^CG[CT]C
3949 GACGTC
AflIII 1338 ACACGT A^C[GA][CT]GT
2455 ACATGT
AgeI 1401 ACCGGT A^CCGGT
2467 ACCGGT
AocI 3163 CCTCAGG CC^T[ACGT]AGG
4002 CCTCAGG
BbvCI 1297 CCTCAGC CCTCAGC(-5/-2)
1702 CCTCAGC
BsaHI 1927 GGCGTC G[GA]^CG[CT]C
3949 GACGTC
BsaWI 1401 ACCGGT [AT]^CCGG[AT]
2467 ACCGGT
BsgI 1306 CTGCAC GTGCAG(16/14)
1612 CTGCAC
BstXI 749 CCAGACAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2747 CCAGGAAGATGG
Bsu36I 3163 CCTCAGG CC^T[ACGT]AGG
4002 CCTCAGG
EaeI 1029 CGGCCA [CT]^GGCC[GA]
1136 TGGCCA
Eco81I 3163 CCTCAGG CC^T[ACGT]AGG
4002 CCTCAGG
EcoT22I 337 ATGCAT ATGCA^T
3330 ATGCAT
HgaI 1928 GCGTC GACGC(5/10)
3554 GCGTC
Hin1I 1927 GGCGTC G[GA]^CG[CT]C
3949 GACGTC
HindIII 1794 AAGCTT A^AGCTT
3723 AAGCTT
NsiI 337 ATGCAT ATGCA^T
3330 ATGCAT
PinAI 1401 ACCGGT A^CCGGT
2467 ACCGGT
SmaI 2222 CCCGGG CCC^GGG
2878 CCCGGG
SphI 688 GCATGC GCATG^C
1744 GCATGC
XmaCI 2222 CCCGGG C^CCGGG
2878 CCCGGG
XmaI 2222 CCCGGG C^CCGGG
2878 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 100 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI AlwNI ApoI Asp700I AspHI
AvaI AvaII BamHI BanII BbsI
BbvI BciVI BcnI BfaI BmyI
BpmI BpuAI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrFI BsrI BssKI
BstF5I BstNI BstYI Cac8I Cfr10I
Cfr13I DdeI DpnII DraII DraIII
DsaI EarI EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MspA1I
MspI MvaI MwoI NciI NcoI
NdeII NheI NlaIII NlaIV NspI
PleI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI XhoII XmnI