##################################
# NO CUT ENZYMES ( 54 enzymes) #
##################################
AatII AclI AflII AscI AseI
AsnI AspI AviII AvrII BfrI
BlnI BsiEI BsiWI BspHI Bst1107I
BstBI BstZ17I CpoI DraI DrdI
EagI EclXI Eco52I EcoRV FseI
FspI HincII HindII HpaI MfeI
MluI MunI NdeI NotI NruI
NspV PacI PaeR7I PflFI PmeI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SalI SfiI SfuI SgrAI
SpeI SwaI Tth111I XhoI
###################################
# ONE CUT ENZYMES ( 57 enzymes) #
###################################
Acc65I 2496 GGTACC G^GTACC
AccI 3464 GTCTAC GT^[AC][GT]AC
AccIII 3354 TCCGGA T^CCGGA
AgeI 2640 ACCGGT A^CCGGT
Aor51HI 1346 AGCGCT AGC^GCT
Asp718I 2496 GGTACC G^GTACC
BalI 696 TGGCCA TGG^CCA
BbeI 477 GGCGCC GGCGC^C
BbrPI 1274 CACGTG CAC^GTG
BciVI 2967 GTATCC GTATCC(6/5)
BglI 1537 GCCGCTGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 2488 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2488 GCTGAGC GC^T[ACGT]AGC
BsaBI 1102 GATCCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 2015 GAGACC GGTCTC(1/5)
BseAI 3354 TCCGGA T^CCGGA
BspDI 2029 ATCGAT AT^CGAT
BspEI 3354 TCCGGA T^CCGGA
BspLU11I 552 ACATGT A^CATGT
BstEII 1522 GGTGACC G^GT[ACGT]ACC
BstPI 1522 GGTGACC G^GT[ACGT]ACC
CelII 2488 GCTGAGC GC^T[ACGT]AGC
ClaI 2029 ATCGAT AT^CGAT
DraIII 2543 CACAACGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 696 TGGCCA [CT]^GGCC[GA]
Eco47III 1346 AGCGCT AGC^GCT
EcoO65I 1522 GGTGACC G^GT[ACGT]ACC
EcoRI 3675 GAATTC G^AATTC
HindIII 1298 AAGCTT A^AGCTT
KasI 477 GGCGCC G^GCGCC
KpnI 2496 GGTACC GGTAC^C
KspI 38 CCGCGG CCGC^GG
MamI 1102 GATCCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 696 TGGCCA TGG^CCA
MroI 3354 TCCGGA T^CCGGA
MscI 696 TGGCCA TGG^CCA
NaeI 2808 GCCGGC GCC^GGC
NarI 477 GGCGCC GG^CGCC
NcoI 1947 CCATGG C^CATGG
NgoMIV 2808 GCCGGC G^CCGGC
NheI 2170 GCTAGC G^CTAGC
PflMI 2005 CCACATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 2640 ACCGGT A^CCGGT
PmaCI 1274 CACGTG CAC^GTG
PmlI 1274 CACGTG CAC^GTG
SacII 38 CCGCGG CCGC^GG
SexAI 2093 ACCAGGT A^CC[AT]GGT
SfoI 477 GGCGCC GGC^GCC
SmaI 324 CCCGGG CCC^GGG
SnaBI 639 TACGTA TAC^GTA
SphI 3849 GCATGC GCATG^C
SspI 4019 AATATT AAT^ATT
StuI 1432 AGGCCT AGG^CCT
Van91I 2005 CCACATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 3973 TCTAGA T^CTAGA
XmaCI 324 CCCGGG C^CCGGG
XmaI 324 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AflIII 552 ACATGT A^C[GA][CT]GT
2547 ACGTGT
AhdI 80 GACGCGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
506 GACTTCGTGTC
Alw44I 1054 GTGCAC G^TGCAC
1304 GTGCAC
AocI 404 CCTGAGG CC^T[ACGT]AGG
1528 CCTTAGG
ApaI 13 GGGCCC GGGCC^C
1811 GGGCCC
ApaLI 1054 GTGCAC G^TGCAC
1304 GTGCAC
Asp700I 502 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1832 GAAAAATTTC
AspEI 80 GACGCGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
506 GACTTCGTGTC
AvaI 324 CCCGGG C^[CT]CG[GA]G
1177 CTCGGG
BanI 477 GGCGCC G^G[CT][GA]CC
2496 GGTACC
BbvCI 3260 CCTCAGC CCTCAGC(-5/-2)
3813 GCTGAGG
BglII 2928 AGATCT A^GATCT
4374 AGATCT
BsmI 2243 GAATGC GAATGC(1/-1)
3471 GCATTC
BsoBI 324 CCCGGG C^[CT]CG[GA]G
1177 CTCGGG
Bsp1407I 303 TGTACA T^GTACA
2064 TGTACA
BsrBI 627 CCGCTC CCGCTC(-3/-3)
1675 GAGCGG
BsrDI 1762 CATTGC GCAATG(2/0)
3399 GCAATG
BsrGI 303 TGTACA T^GTACA
2064 TGTACA
BssHII 1145 GCGCGC G^CGCGC
1269 GCGCGC
BssSI 2948 CACGAG CACGAG(-5/-1)
3488 CACGAG
BstAPI 1240 GCAGGGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3820 GCACGCGCTGC
BstXI 687 CCATGTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1815 CCACCATATTGG
Bsu36I 404 CCTGAGG CC^T[ACGT]AGG
1528 CCTTAGG
Eam1105I 80 GACGCGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
506 GACTTCGTGTC
Eco81I 404 CCTGAGG CC^T[ACGT]AGG
1528 CCTTAGG
SacI 761 GAGCTC GAGCT^C
2591 GAGCTC
SapI 758 GAAGAGC GCTCTTC(1/4)
1222 GCTCTTC
SbfI 1435 CCTGCAGG CCTGCA^GG
1585 CCTGCAGG
ScaI 945 AGTACT AGT^ACT
1320 AGTACT
Sse8387I 1435 CCTGCAGG CCTGCA^GG
1585 CCTGCAGG
SspBI 303 TGTACA T^GTACA
2064 TGTACA
XcmI 2436 CCATAGTTGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3613 CCAGAAACATGATGG
XmnI 502 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1832 GAAAAATTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 114 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApoI AspHI
AvaII BamHI BanII BbsI BbvI
BcgI BclI BcnI BfaI BmyI
BpmI BpuAI BsaAI BsaHI BsaJI
BsaWI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI Bsp1286I
BspMI BsrFI BsrI BssKI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EarI EcoNI EcoO109I EcoRII
EcoT14I EcoT22I FauI FbaI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NsiI NspI PleI PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII