##################################
# NO CUT ENZYMES ( 106 enzymes) #
##################################
AatII Acc65I AccII AclI AflII
AflIII AgeI AhdI Alw44I AlwNI
ApaI ApaLI AscI Asp718I AspEI
AspI AvaI AviII AvrII BbeI
BbrPI BbvCI BcgI BciVI BfrI
BglII BlnI BsaAI BsaI BsiWI
BsmFI BsoBI Bsp1407I BspDI BspLU11I
BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstUI Cfr10I ClaI CpoI
DraI DraIII DrdI Eam1105I EcoNI
EcoT22I FauI FseI FspI HpaI
KasI KpnI KspI MaeII MfeI
MluI MunI MvnI NaeI NarI
NcoI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI Psp1406I PvuI RsrII
SacII SalI SbfI SexAI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI Tth111I Van91I XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 60 enzymes) #
###################################
AccIII 979 TCCGGA T^CCGGA
AocI 210 CCTGAGG CC^T[ACGT]AGG
Aor51HI 1465 AGCGCT AGC^GCT
AseI 528 ATTAAT AT^TAAT
AsnI 528 ATTAAT AT^TAAT
AspHI 138 GAGCTC G[AT]GC[AT]^C
BamHI 406 GGATCC G^GATCC
BanI 1438 GGCACC G^G[CT][GA]CC
BclI 1403 TGATCA T^GATCA
BpmI 910 CTCCAG CTGGAG(16/14)
BsaBI 824 GATGAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 979 TCCGGA [AT]^CCGG[AT]
BseAI 979 TCCGGA T^CCGGA
BseRI 457 GAGGAG GAGGAG(10/8)
BsgI 24 GTGCAG GTGCAG(16/14)
BsiHKAI 138 GAGCTC G[AT]GC[AT]^C
BsmBI 86 GAGACG CGTCTC(1/5)
BsmI 1311 GAATGC GAATGC(1/-1)
BspEI 979 TCCGGA T^CCGGA
BspHI 1483 TCATGA T^CATGA
BsrDI 1501 GCAATG GCAATG(2/0)
Bst1107I 1508 GTATAC GTA^TAC
BstEII 1124 GGTCACC G^GT[ACGT]ACC
BstPI 1124 GGTCACC G^GT[ACGT]ACC
BstXI 1133 CCACCCAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 406 GGATCC [GA]^GATC[CT]
BstZ17I 1508 GTATAC GTA^TAC
Bsu36I 210 CCTGAGG CC^T[ACGT]AGG
CfoI 1466 GCGC GCG^C
DraII 206 GGGTCCT [GA]G^G[ACGT]CC[CT]
DsaI 847 CCGTGG C^C[GA][CT]GG
Eco47III 1465 AGCGCT AGC^GCT
Eco81I 210 CCTGAGG CC^T[ACGT]AGG
EcoO109I 206 GGGTCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 1124 GGTCACC G^GT[ACGT]ACC
EcoRI 1878 GAATTC G^AATTC
EcoRV 1287 GATATC GAT^ATC
FbaI 1403 TGATCA T^GATCA
HaeII 1465 AGCGCT [GA]GCGC^[CT]
HhaI 1466 GCGC GCG^C
HinP1I 1466 GCGC G^CGC
HincII 1167 GTTGAC GT[CT]^[GA]AC
HindII 1167 GTTGAC GT[CT]^[GA]AC
HindIII 1350 AAGCTT A^AGCTT
MamI 824 GATGAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 406 GGATCC [GA]^GATC[CT]
MroI 979 TCCGGA T^CCGGA
MslI 1134 CACCCAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NdeI 645 CATATG CA^TATG
PpuMI 206 GGGTCCT [GA]G^G[AT]CC[CT]
PshBI 528 ATTAAT AT^TAAT
RcaI 1483 TCATGA T^CATGA
SacI 138 GAGCTC GAGCT^C
SapI 1206 GAAGAGC GCTCTTC(1/4)
ScaI 1712 AGTACT AGT^ACT
SfiI 1143 GGCCCAGAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI 1212 CTTGAG C^T[CT][GA]AG
XbaI 1804 TCTAGA T^CTAGA
XcmI 1765 CCACTGATACACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 406 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AccI 315 GTAGAC GT^[AC][GT]AC
1508 GTATAC
AfaI 1615 GTAC GT^AC
1713 GTAC
Asp700I 516 GAATTTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1710 GAAGTACTTC
BalI 484 TGGCCA TGG^CCA
850 TGGCCA
BanII 138 GAGCTC G[GA]GC[CT]^C
1421 GAGCCC
BbsI 288 GAAGAC GAAGAC(2/6)
1740 GAAGAC
BcnI 7 CCGGG CC^[GC]GG
953 CCGGG
BfaI 804 CTAG C^TAG
1805 CTAG
BglI 21 GCCGTGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1144 GCCCAGAGGGC
BlpI 155 GCTAAGC GC^T[ACGT]AGC
599 GCTGAGC
BmyI 138 GAGCTC G[AGT]GC[ACT]^C
1421 GAGCCC
Bpu1102I 155 GCTAAGC GC^T[ACGT]AGC
599 GCTGAGC
BpuAI 288 GAAGAC GAAGAC(2/6)
1740 GAAGAC
Bsp1286I 138 GAGCTC G[AGT]GC[ACT]^C
1421 GAGCCC
BsrBI 752 CCGCTC CCGCTC(-3/-3)
1442 CCGCTC
BsrI 1775 ACTGG ACTGG(1/-1)
974 CCAGT
CelII 155 GCTAAGC GC^T[ACGT]AGC
599 GCTGAGC
EagI 19 CGGCCG C^GGCCG
31 CGGCCG
EclXI 19 CGGCCG C^GGCCG
31 CGGCCG
Eco52I 19 CGGCCG C^GGCCG
31 CGGCCG
EcoT14I 1561 CCAAGG C^C[AT][AT]GG
1567 CCAAGG
MaeI 804 CTAG C^TAG
1805 CTAG
MluNI 484 TGGCCA TGG^CCA
850 TGGCCA
MscI 484 TGGCCA TGG^CCA
850 TGGCCA
NciI 7 CCGGG CC^[GC]GG
953 CCGGG
PleI 1692 GACTC GAGTC(4/5)
1672 GAGTC
PstI 357 CTGCAG CTGCA^G
1573 CTGCAG
PvuII 270 CAGCTG CAG^CTG
1645 CAGCTG
RsaI 1615 GTAC GT^AC
1713 GTAC
SfcI 357 CTGCAG C^T[GA][CT]AG
1573 CTGCAG
StyI 1561 CCAAGG C^C[AT][AT]GG
1567 CCAAGG
XmnI 516 GAATTTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1710 GAAGTACTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 60 enzymes) #
#########################################
AciI AcsI AcyI AluI AlwI
ApoI AvaII BbvI BsaHI BsaJI
BsiEI BsiYI BslI BsmAI BspMI
BssKI BstF5I BstNI Cac8I Cfr13I
DdeI DpnII EaeI EarI EcoRII
Fnu4HI FokI HaeIII HapII HgaI
Hin1I HinfI HpaII HphI ItaI
Ksp632I MaeIII MboI MboII MnlI
MseI MspA1I MspI MvaI MwoI
NdeII NlaIII NlaIV Sau3AI Sau96I
ScrFI SfaNI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I