##################################
# NO CUT ENZYMES ( 62 enzymes) #
##################################
AatII AclI AcyI AhdI Aor51HI
ApaI AscI AspEI AviII BbeI
BbrPI BcgI BglI BsaAI BsaHI
BsiEI BsiWI BsmBI BspDI BsrBI
BssHII BstAPI BstEII BstPI ClaI
CpoI DraIII EagI Eam1105I EclXI
Eco47III Eco52I EcoO65I FseI FspI
HgaI Hin1I KasI KspI MluI
NaeI NarI NgoMIV NheI NotI
NruI PacI PmaCI PmeI PmlI
Psp1406I PvuI RsrII SacII SbfI
SexAI SfiI SfoI SgrAI SnaBI
Sse8387I SwaI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
Acc65I 5453 GGTACC G^GTACC
AccII 1760 CGCG CG^CG
AccIII 5588 TCCGGA T^CCGGA
AflIII 1752 ACATGT A^C[GA][CT]GT
AgeI 1100 ACCGGT A^CCGGT
Asp718I 5453 GGTACC G^GTACC
AspI 5637 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 343 CCTAGG C^CTAGG
BbvCI 5249 CCTCAGC CCTCAGC(-5/-2)
BlnI 343 CCTAGG C^CTAGG
BseAI 5588 TCCGGA T^CCGGA
BseRI 5216 CTCCTC GAGGAG(10/8)
BspEI 5588 TCCGGA T^CCGGA
BspLU11I 1752 ACATGT A^CATGT
BsrFI 1100 ACCGGT [GA]^CCGG[CT]
BssSI 392 CTCGTG CACGAG(-5/-1)
Bst1107I 2419 GTATAC GTA^TAC
BstBI 3465 TTCGAA TT^CGAA
BstUI 1760 CGCG CG^CG
BstXI 2251 CCAGCATTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 2419 GTATAC GTA^TAC
Cfr10I 1100 ACCGGT [GA]^CCGG[CT]
DrdI 3885 GACGTTTTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 1681 CCTTCAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HaeII 906 GGCGCT [GA]GCGC^[CT]
HpaI 1000 GTTAAC GTT^AAC
KpnI 5453 GGTACC GGTAC^C
MroI 5588 TCCGGA T^CCGGA
MvnI 1760 CGCG CG^CG
NspV 3465 TTCGAA TT^CGAA
PflFI 5637 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 1100 ACCGGT A^CCGGT
PshAI 5695 GACCAGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SalI 4254 GTCGAC G^TCGAC
SfuI 3465 TTCGAA TT^CGAA
SphI 4831 GCATGC GCATG^C
Tth111I 5637 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 50 enzymes) #
###################################
AflII 2899 CTTAAG C^TTAAG
4990 CTTAAG
AocI 3283 CCTAAGG CC^T[ACGT]AGG
5448 CCTCAGG
AseI 577 ATTAAT AT^TAAT
4747 ATTAAT
AsnI 577 ATTAAT AT^TAAT
4747 ATTAAT
BalI 3023 TGGCCA TGG^CCA
3701 TGGCCA
BanII 2237 GAGCTC G[GA]GC[CT]^C
5500 GAGCTC
BbsI 3851 GAAGAC GAAGAC(2/6)
1624 GTCTTC
BciVI 3689 GGATAC GTATCC(6/5)
3495 GTATCC
BfrI 2899 CTTAAG C^TTAAG
4990 CTTAAG
BglII 2773 AGATCT A^GATCT
5531 AGATCT
BpuAI 3851 GAAGAC GAAGAC(2/6)
1624 GTCTTC
BsaBI 677 GATTGGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3472 GATGGCTATC
BsaWI 1100 ACCGGT [AT]^CCGG[AT]
5588 TCCGGA
BsgI 53 GTGCAG GTGCAG(16/14)
4617 GTGCAG
BsmI 3271 GAATGC GAATGC(1/-1)
5546 GCATTC
Bsp1407I 1054 TGTACA T^GTACA
3013 TGTACA
BspMI 3339 ACCTGC ACCTGC(4/8)
1733 GCAGGT
BsrDI 1329 CATTGC GCAATG(2/0)
3056 GCAATG
BsrGI 1054 TGTACA T^GTACA
3013 TGTACA
Bsu36I 3283 CCTAAGG CC^T[ACGT]AGG
5448 CCTCAGG
CfoI 907 GCGC GCG^C
1761 GCGC
EaeI 3023 TGGCCA [CT]^GGCC[GA]
3701 TGGCCA
Eco81I 3283 CCTAAGG CC^T[ACGT]AGG
5448 CCTCAGG
EcoRV 2873 GATATC GAT^ATC
4919 GATATC
FauI 225 CCCGC CCCGC(4/6)
1257 CCCGC
HhaI 907 GCGC GCG^C
1761 GCGC
HinP1I 907 GCGC G^CGC
1761 GCGC
HincII 1000 GTTAAC GT[CT]^[GA]AC
4254 GTCGAC
HindII 1000 GTTAAC GT[CT]^[GA]AC
4254 GTCGAC
MamI 677 GATTGGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3472 GATGGCTATC
MfeI 3132 CAATTG C^AATTG
3456 CAATTG
MluNI 3023 TGGCCA TGG^CCA
3701 TGGCCA
MscI 3023 TGGCCA TGG^CCA
3701 TGGCCA
MunI 3132 CAATTG C^AATTG
3456 CAATTG
NdeI 687 CATATG CA^TATG
1304 CATATG
PaeR7I 1917 CTCGAG C^TCGAG
2445 CTCGAG
PpuMI 1294 AGGTCCT [GA]G^G[AT]CC[CT]
5760 AGGTCCT
PshBI 577 ATTAAT AT^TAAT
4747 ATTAAT
SacI 2237 GAGCTC GAGCT^C
5500 GAGCTC
SapI 1865 GAAGAGC GCTCTTC(1/4)
3734 GAAGAGC
ScaI 4329 AGTACT AGT^ACT
4846 AGTACT
SmaI 74 CCCGGG CCC^GGG
2141 CCCGGG
SpeI 628 ACTAGT A^CTAGT
5406 ACTAGT
SspBI 1054 TGTACA T^GTACA
3013 TGTACA
StuI 2903 AGGCCT AGG^CCT
3593 AGGCCT
Tsp45I 5377 GTCAC ^GT[GC]AC
5643 GTCAC
XbaI 1108 TCTAGA T^CTAGA
2282 TCTAGA
XhoI 1917 CTCGAG C^TCGAG
2445 CTCGAG
XmaCI 74 CCCGGG C^CCGGG
2141 CCCGGG
XmaI 74 CCCGGG C^CCGGG
2141 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 109 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
Alw44I AlwI AlwNI ApaLI ApoI
Asp700I AspHI AvaI AvaII BamHI
BanI BbvI BclI BcnI BfaI
BlpI BmyI BpmI Bpu1102I BsaI
BsaJI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspHI BsrI
BssKI BstF5I BstNI BstYI Cac8I
CelII Cfr13I DdeI DpnII DraI
DraII DsaI EarI EcoO109I EcoRI
EcoRII EcoT14I EcoT22I FbaI Fnu4HI
FokI HaeIII HapII HindIII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MwoI NciI NcoI NdeII
NlaIII NlaIV NsiI NspI PflMI
PleI PstI PvuII RcaI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspI StyI TaqI TfiI
Tru9I TseI Tsp509I TspRI TthHB8I
Van91I XcmI XhoII XmnI