##################################
# NO CUT ENZYMES ( 88 enzymes) #
##################################
AatII Acc65I AccIII AclI AgeI
AhdI Alw44I Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AviII AvrII BbrPI BcgI BciVI
BglI BglII BlnI BsaAI BsaI
BsaWI BseAI BsiEI BsiWI BsmBI
BspEI BsrBI BsrDI BssHII BssSI
BstXI CpoI DraIII DrdI DsaI
EagI Eam1105I EclXI Eco47III Eco52I
EcoRV FseI FspI HgaI HindIII
KpnI KspI MaeII MfeI MluI
MroI MunI NcoI NdeI NotI
NruI PacI PaeR7I PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI PvuII RsrII
SacI SacII SalI SbfI SfiI
SgrAI SnaBI SphI Sse8387I SwaI
XbaI XcmI XhoI
###################################
# ONE CUT ENZYMES ( 70 enzymes) #
###################################
AccI 3314 GTATAC GT^[AC][GT]AC
AccII 2643 CGCG CG^CG
AcyI 17 GGCGCC G[GA]^CG[CT]C
AflII 3256 CTTAAG C^TTAAG
AflIII 726 ACATGT A^C[GA][CT]GT
Asp700I 518 GAAGAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 1163 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 112 CCCGGG C^[CT]CG[GA]G
BalI 225 TGGCCA TGG^CCA
BamHI 1027 GGATCC G^GATCC
BbeI 17 GGCGCC GGCGC^C
BbvCI 1655 CCTCAGC CCTCAGC(-5/-2)
BfrI 3256 CTTAAG C^TTAAG
BlpI 1313 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1313 GCTCAGC GC^T[ACGT]AGC
BsaHI 17 GGCGCC G[GA]^CG[CT]C
BsmAI 1277 GAGAC GTCTC(1/5)
BsmFI 2145 GGGAC GGGAC(10/14)
BsmI 425 GCATTC GAATGC(1/-1)
BsoBI 112 CCCGGG C^[CT]CG[GA]G
Bsp1407I 2183 TGTACA T^GTACA
BspLU11I 726 ACATGT A^CATGT
BsrFI 20 GCCGGC [GA]^CCGG[CT]
BsrGI 2183 TGTACA T^GTACA
Bst1107I 3314 GTATAC GTA^TAC
BstAPI 2981 GCAGACGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 375 TTCGAA TT^CGAA
BstEII 2589 GGTCACC G^GT[ACGT]ACC
BstPI 2589 GGTCACC G^GT[ACGT]ACC
BstUI 2643 CGCG CG^CG
BstZ17I 3314 GTATAC GTA^TAC
CelII 1313 GCTCAGC GC^T[ACGT]AGC
CfoI 18 GCGC GCG^C
Cfr10I 20 GCCGGC [GA]^CCGG[CT]
DraII 217 AGGGCCT [GA]G^G[ACGT]CC[CT]
EcoO109I 217 AGGGCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 2589 GGTCACC G^GT[ACGT]ACC
EcoRI 1427 GAATTC G^AATTC
EcoT22I 1812 ATGCAT ATGCA^T
HaeII 17 GGCGCC [GA]GCGC^[CT]
HhaI 18 GCGC GCG^C
Hin1I 17 GGCGCC G[GA]^CG[CT]C
HinP1I 18 GCGC G^CGC
KasI 17 GGCGCC G^GCGCC
MluNI 225 TGGCCA TGG^CCA
MscI 225 TGGCCA TGG^CCA
MslI 562 CATCGATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 139 CAGCGG C[AC]G^C[GT]G
MvnI 2643 CGCG CG^CG
NaeI 20 GCCGGC GCC^GGC
NarI 17 GGCGCC GG^CGCC
NgoMIV 20 GCCGGC G^CCGGC
NheI 238 GCTAGC G^CTAGC
NsiI 1812 ATGCAT ATGCA^T
NspV 375 TTCGAA TT^CGAA
PflFI 1163 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 228 CCAAAATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SapI 1520 GAAGAGC GCTCTTC(1/4)
ScaI 3033 AGTACT AGT^ACT
SfoI 17 GGCGCC GGC^GCC
SfuI 375 TTCGAA TT^CGAA
SmaI 112 CCCGGG CCC^GGG
SpeI 2114 ACTAGT A^CTAGT
SspBI 2183 TGTACA T^GTACA
StuI 2803 AGGCCT AGG^CCT
Tth111I 1163 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 228 CCAAAATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 112 CCCGGG C^CCGGG
XmaI 112 CCCGGG C^CCGGG
XmnI 518 GAAGAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AlwNI 539 CAGCCACTG CAG[ACGT][ACGT][ACGT]^CTG
1638 CAGAGCCTG
AspHI 775 GAGCAC G[AT]GC[AT]^C
1890 GTGCTC
AvaII 1468 GGACC G^G[AT]CC
2057 GGACC
BanI 17 GGCGCC G^G[CT][GA]CC
1243 GGCACC
BbsI 1379 GAAGAC GAAGAC(2/6)
1881 GTCTTC
BclI 535 TGATCA T^GATCA
1057 TGATCA
BcnI 112 CCCGG CC^[GC]GG
113 CCGGG
BpmI 1535 CTCCAG CTGGAG(16/14)
1905 CTGGAG
BpuAI 1379 GAAGAC GAAGAC(2/6)
1881 GTCTTC
BsaBI 1826 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2396 GATTTTTATC
BsgI 960 CTGCAC GTGCAG(16/14)
3056 CTGCAC
BsiHKAI 775 GAGCAC G[AT]GC[AT]^C
1890 GTGCTC
BspDI 563 ATCGAT AT^CGAT
1502 ATCGAT
BstYI 1027 GGATCC [GA]^GATC[CT]
1237 GGATCT
ClaI 563 ATCGAT AT^CGAT
1502 ATCGAT
EaeI 225 TGGCCA [CT]^GGCC[GA]
452 CGGCCA
EcoNI 469 CCTGCCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
925 CCTCTCTGAGG
FauI 126 CCCGC CCCGC(4/6)
36 GCGGG
FbaI 535 TGATCA T^GATCA
1057 TGATCA
HapII 21 CCGG C^CGG
113 CCGG
HpaI 2549 GTTAAC GTT^AAC
3236 GTTAAC
HpaII 21 CCGG C^CGG
113 CCGG
MamI 1826 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2396 GATTTTTATC
MflI 1027 GGATCC [GA]^GATC[CT]
1237 GGATCT
MspI 21 CCGG C^CGG
113 CCGG
NciI 112 CCCGG CC^[GC]GG
113 CCGGG
NspI 726 ACATGT [GA]CATG^[CT]
2361 GCATGT
SexAI 2694 ACCTGGT A^CC[AT]GGT
2959 ACCTGGT
SfcI 1686 CTGTAG C^T[GA][CT]AG
1975 CTACAG
XhoII 1027 GGATCC [GA]^GATC[CT]
1237 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 70 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AocI ApoI BanII BbvI BfaI
BmyI BsaJI BseRI BsiYI BslI
Bsp1286I BspHI BspMI BsrI BssKI
BstF5I BstNI Bsu36I Cac8I Cfr13I
DdeI DpnII DraI EarI Eco81I
EcoRII EcoT14I Fnu4HI FokI HaeIII
HincII HindII HinfI HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MnlI MseI MvaI MwoI NdeII
NlaIII NlaIV PleI RcaI RsaI
Sau3AI Sau96I ScrFI SfaNI SmlI
SspI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I