##################################
# NO CUT ENZYMES ( 67 enzymes) #
##################################
AclI AflII AgeI AhdI Aor51HI
AscI AseI AsnI AspEI AviII
BbeI BbrPI BfrI BsaAI BsiWI
BspDI BssHII Bst1107I BstBI BstZ17I
ClaI CpoI DraI DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoRV
FseI FspI HpaI KasI KspI
MfeI MluI MunI NaeI NarI
NgoMIV NotI NruI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PshBI Psp1406I RsrII SacII SalI
SbfI SexAI SfoI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SwaI
XbaI XhoI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AatII 1639 GACGTC GACGT^C
Acc65I 4048 GGTACC G^GTACC
AccI 2272 GTCTAC GT^[AC][GT]AC
AccII 1750 CGCG CG^CG
AccIII 2378 TCCGGA T^CCGGA
AcsI 1567 GAATTC [GA]^AATT[CT]
AcyI 1639 GACGTC G[GA]^CG[CT]C
ApoI 1567 GAATTC [GA]^AATT[CT]
Asp700I 102 GAATGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 4048 GGTACC G^GTACC
AspI 2976 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbvCI 1051 CCTCAGC CCTCAGC(-5/-2)
BcgI 2496 GCATGACGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 365 GCCAGTTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 1276 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 1276 GCTAAGC GC^T[ACGT]AGC
BsaHI 1639 GACGTC G[GA]^CG[CT]C
BseAI 2378 TCCGGA T^CCGGA
BsiEI 2502 CGATCG CG[GA][CT]^CG
BspEI 2378 TCCGGA T^CCGGA
BspHI 1631 TCATGA T^CATGA
BspMI 2327 ACCTGC ACCTGC(4/8)
BsrFI 1513 ACCGGC [GA]^CCGG[CT]
BssSI 4171 CACGAG CACGAG(-5/-1)
BstEII 4003 GGTGACC G^GT[ACGT]ACC
BstPI 4003 GGTGACC G^GT[ACGT]ACC
BstUI 1750 CGCG CG^CG
CelII 1276 GCTAAGC GC^T[ACGT]AGC
Cfr10I 1513 ACCGGC [GA]^CCGG[CT]
EcoO65I 4003 GGTGACC G^GT[ACGT]ACC
EcoRI 1567 GAATTC G^AATTC
EcoT22I 4422 ATGCAT ATGCA^T
Hin1I 1639 GACGTC G[GA]^CG[CT]C
HindIII 3256 AAGCTT A^AGCTT
KpnI 4048 GGTACC GGTAC^C
MroI 2378 TCCGGA T^CCGGA
MvnI 1750 CGCG CG^CG
NsiI 4422 ATGCAT ATGCA^T
PflFI 2976 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PvuI 2502 CGATCG CGAT^CG
RcaI 1631 TCATGA T^CATGA
ScaI 2995 AGTACT AGT^ACT
SfiI 364 GGCCAGTTTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SspI 3710 AATATT AAT^ATT
Tth111I 2976 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 102 GAATGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
Alw44I 512 GTGCAC G^TGCAC
3840 GTGCAC
ApaLI 512 GTGCAC G^TGCAC
3840 GTGCAC
AvrII 1717 CCTAGG C^CTAGG
4105 CCTAGG
BamHI 707 GGATCC G^GATCC
3771 GGATCC
BciVI 2393 GTATCC GTATCC(6/5)
3555 GTATCC
BglII 2196 AGATCT A^GATCT
4036 AGATCT
BlnI 1717 CCTAGG C^CTAGG
4105 CCTAGG
BsaBI 1238 GATGTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2623 GATCTATATC
BsaI 2938 GAGACC GGTCTC(1/5)
431 GGTCTC
BsaWI 2378 TCCGGA [AT]^CCGG[AT]
4007 ACCGGA
BsmBI 1641 CGTCTC CGTCTC(1/5)
3135 CGTCTC
Bsp1407I 384 TGTACA T^GTACA
1240 TGTACA
BspLU11I 1406 ACATGT A^CATGT
1847 ACATGT
BsrBI 2311 GAGCGG CCGCTC(-3/-3)
2520 GAGCGG
BsrGI 384 TGTACA T^GTACA
1240 TGTACA
BstAPI 417 GCAAAGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1388 GCAACCGCTGC
DraIII 2730 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
3972 CACTCCGTG
HaeII 1070 AGCGCC [GA]GCGC^[CT]
4231 AGCGCC
HincII 121 GTCAAC GT[CT]^[GA]AC
1879 GTCAAC
HindII 121 GTCAAC GT[CT]^[GA]AC
1879 GTCAAC
MamI 1238 GATGTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2623 GATCTATATC
NcoI 1362 CCATGG C^CATGG
2924 CCATGG
NdeI 359 CATATG CA^TATG
3364 CATATG
NheI 2773 GCTAGC G^CTAGC
3429 GCTAGC
NspI 1406 ACATGT [GA]CATG^[CT]
1847 ACATGT
PpuMI 445 GGGTCCC [GA]G^G[AT]CC[CT]
771 GGGTCCT
SapI 1797 GAAGAGC GCTCTTC(1/4)
3028 GAAGAGC
SmaI 980 CCCGGG CCC^GGG
4195 CCCGGG
SspBI 384 TGTACA T^GTACA
1240 TGTACA
StuI 740 AGGCCT AGG^CCT
3213 AGGCCT
XmaCI 980 CCCGGG C^CCGGG
4195 CCCGGG
XmaI 980 CCCGGG C^CCGGG
4195 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 113 enzymes) #
#########################################
AciI AfaI AflIII AluI AlwI
AlwNI AocI ApaI AspHI AvaI
AvaII BalI BanI BanII BbsI
BbvI BclI BcnI BfaI BmyI
BpmI BpuAI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I BsrDI BsrI
BssKI BstF5I BstNI BstXI BstYI
Bsu36I Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
Eco81I EcoNI EcoO109I EcoRII EcoT14I
FauI FbaI Fnu4HI FokI HaeIII
HapII HgaI HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PflMI PleI
PshAI PstI PvuII RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII