##################################
# NO CUT ENZYMES ( 59 enzymes) #
##################################
AatII Acc65I AccIII AflIII AhdI
Aor51HI AscI Asp700I Asp718I AspEI
AspI AviII AvrII BbeI BbrPI
BcgI BlnI BsaAI BseAI BsiWI
BsmBI BspDI BspEI BspLU11I BsrBI
BssHII ClaI EagI Eam1105I EclXI
Eco47III Eco52I FspI HindIII KasI
KpnI KspI MfeI MluI MroI
MunI NarI NheI NotI NruI
PaeR7I PflFI PmaCI PmeI PmlI
SacII SalI SfiI SfoI SnaBI
StuI Tth111I XhoI XmnI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AcyI 94 GGCGTC G[GA]^CG[CT]C
AflII 46 CTTAAG C^TTAAG
ApaI 2924 GGGCCC GGGCC^C
BamHI 697 GGATCC G^GATCC
BciVI 104 GGATAC GTATCC(6/5)
BclI 3494 TGATCA T^GATCA
BfrI 46 CTTAAG C^TTAAG
BglI 4901 GCCATGTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1801 AGATCT A^GATCT
BsaHI 94 GGCGTC G[GA]^CG[CT]C
BsiEI 1374 CGATCG CG[GA][CT]^CG
BspHI 5273 TCATGA T^CATGA
BssSI 1887 CACGAG CACGAG(-5/-1)
Bst1107I 5629 GTATAC GTA^TAC
BstBI 4921 TTCGAA TT^CGAA
BstEII 315 GGTGACC G^GT[ACGT]ACC
BstPI 315 GGTGACC G^GT[ACGT]ACC
BstZ17I 5629 GTATAC GTA^TAC
CpoI 79 CGGACCG CG^G[AT]CCG
DrdI 2381 GACCTCAAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 315 GGTGACC G^GT[ACGT]ACC
FbaI 3494 TGATCA T^GATCA
FseI 273 GGCCGGCC GGCCGG^CC
HaeII 1721 GGCGCT [GA]GCGC^[CT]
Hin1I 94 GGCGTC G[GA]^CG[CT]C
HincII 5867 GTTAAC GT[CT]^[GA]AC
HindII 5867 GTTAAC GT[CT]^[GA]AC
HpaI 5867 GTTAAC GTT^AAC
NaeI 274 GCCGGC GCC^GGC
NdeI 5215 CATATG CA^TATG
NgoMIV 274 GCCGGC G^CCGGC
NspV 4921 TTCGAA TT^CGAA
PshAI 4264 GACAAACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI 1374 CGATCG CGAT^CG
RcaI 5273 TCATGA T^CATGA
RsrII 79 CGGACCG CG^G[AT]CCG
SapI 2489 GCTCTTC GCTCTTC(1/4)
SbfI 4998 CCTGCAGG CCTGCA^GG
SexAI 938 ACCTGGT A^CC[AT]GGT
SfuI 4921 TTCGAA TT^CGAA
SgrAI 263 CACCGGTG C[GA]^CCGG[CT]G
SphI 1892 GCATGC GCATG^C
Sse8387I 4998 CCTGCAGG CCTGCA^GG
SwaI 4113 ATTTAAAT ATTT^AAAT
XcmI 1385 CCAAGAATGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AccI 1228 GTCTAC GT^[AC][GT]AC
5629 GTATAC
AccII 4764 CGCG CG^CG
4858 CGCG
AclI 193 AACGTT AA^CGTT
3209 AACGTT
AgeI 264 ACCGGT A^CCGGT
1192 ACCGGT
Alw44I 1278 GTGCAC G^TGCAC
5016 GTGCAC
ApaLI 1278 GTGCAC G^TGCAC
5016 GTGCAC
BsaBI 1182 GATGGATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2184 GATTTTAATC
BsaWI 264 ACCGGT [AT]^CCGG[AT]
1192 ACCGGT
BsmI 3024 GCATTC GAATGC(1/-1)
4087 GCATTC
Bsp1407I 4548 TGTACA T^GTACA
5475 TGTACA
BspMI 11 ACCTGC ACCTGC(4/8)
5001 GCAGGT
BsrGI 4548 TGTACA T^GTACA
5475 TGTACA
BstUI 4764 CGCG CG^CG
4858 CGCG
DraIII 1421 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
3075 CACATGGTG
EarI 2490 CTCTTC CTCTTC(1/4)
4126 CTCTTC
EcoNI 4332 CCTATGATAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4496 CCTTCTGAAGG
EcoRI 1959 GAATTC G^AATTC
3170 GAATTC
EcoRV 1186 GATATC GAT^ATC
1342 GATATC
HgaI 511 GACGC GACGC(5/10)
95 GCGTC
Ksp632I 2490 CTCTTC CTCTTC(1/4)
4126 CTCTTC
MamI 1182 GATGGATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2184 GATTTTAATC
MvnI 4764 CGCG CG^CG
4858 CGCG
PinAI 264 ACCGGT A^CCGGT
1192 ACCGGT
PpuMI 935 AGGACCT [GA]G^G[AT]CC[CT]
5482 AGGACCC
Psp1406I 193 AACGTT AA^CGTT
3209 AACGTT
SacI 130 GAGCTC GAGCT^C
1102 GAGCTC
ScaI 1511 AGTACT AGT^ACT
5359 AGTACT
SmaI 1542 CCCGGG CCC^GGG
1779 CCCGGG
SpeI 3973 ACTAGT A^CTAGT
5169 ACTAGT
SspBI 4548 TGTACA T^GTACA
5475 TGTACA
SspI 4348 AATATT AAT^ATT
5242 AATATT
XbaI 2391 TCTAGA T^CTAGA
5675 TCTAGA
XmaCI 1542 CCCGGG C^CCGGG
1779 CCCGGG
XmaI 1542 CCCGGG C^CCGGG
1779 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 120 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI AocI ApoI AseI AsnI
AspHI AvaI AvaII BalI BanI
BanII BbsI BbvCI BbvI BcnI
BfaI BlpI BmyI BpmI Bpu1102I
BpuAI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrDI BsrFI BsrI
BssKI BstAPI BstF5I BstNI BstXI
BstYI Bsu36I Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraI
DraII DsaI EaeI Eco81I EcoO109I
EcoRII EcoT14I EcoT22I FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MwoI NciI
NcoI NdeII NlaIII NlaIV NsiI
NspI PacI PflMI PleI PshBI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XhoII