##################################
# NO CUT ENZYMES ( 63 enzymes) #
##################################
AclI AflII AflIII AgeI Alw44I
ApaLI AscI AseI AsnI BbeI
BcgI BciVI BfrI BsiEI BsiWI
BspDI BspLU11I BssHII Bst1107I BstEII
BstPI BstZ17I ClaI CpoI DraI
DrdI EagI EclXI Eco52I EcoO65I
FseI HpaI KasI MfeI MluI
MunI NarI NdeI NheI NotI
NruI PacI PaeR7I PinAI PmeI
PshAI PshBI Psp1406I PvuI RsrII
SalI ScaI SexAI SfiI SfoI
SgrAI SmlI SnaBI SpeI StuI
SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 56 enzymes) #
###################################
Acc65I 3483 GGTACC G^GTACC
AccII 904 CGCG CG^CG
AccIII 1327 TCCGGA T^CCGGA
AlwNI 552 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp718I 3483 GGTACC G^GTACC
AspI 81 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 972 TGCGCA TGC^GCA
BamHI 1762 GGATCC G^GATCC
BbrPI 2936 CACGTG CAC^GTG
BclI 1597 TGATCA T^GATCA
BglII 1972 AGATCT A^GATCT
BsaAI 2936 CACGTG [CT]AC^GT[GA]
BsaWI 1327 TCCGGA [AT]^CCGG[AT]
BseAI 1327 TCCGGA T^CCGGA
BsgI 3580 GTGCAG GTGCAG(16/14)
BsmBI 410 CGTCTC CGTCTC(1/5)
Bsp1407I 2260 TGTACA T^GTACA
BspEI 1327 TCCGGA T^CCGGA
BspHI 3109 TCATGA T^CATGA
BsrGI 2260 TGTACA T^GTACA
BssSI 1777 CTCGTG CACGAG(-5/-1)
BstAPI 204 GCAGAGCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1104 TTCGAA TT^CGAA
BstUI 904 CGCG CG^CG
EcoNI 2905 CCTGAGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 1146 GATATC GAT^ATC
EcoT22I 349 ATGCAT ATGCA^T
FbaI 1597 TGATCA T^GATCA
FspI 972 TGCGCA TGC^GCA
HgaI 409 GCGTC GACGC(5/10)
HincII 1572 GTCAAC GT[CT]^[GA]AC
HindII 1572 GTCAAC GT[CT]^[GA]AC
KpnI 3483 GGTACC GGTAC^C
KspI 903 CCGCGG CCGC^GG
MroI 1327 TCCGGA T^CCGGA
MvnI 904 CGCG CG^CG
NaeI 2533 GCCGGC GCC^GGC
NgoMIV 2533 GCCGGC G^CCGGC
NsiI 349 ATGCAT ATGCA^T
NspV 1104 TTCGAA TT^CGAA
PflFI 81 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 3395 CCAAACATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 2936 CACGTG CAC^GTG
PmlI 2936 CACGTG CAC^GTG
PpuMI 3878 GGGTCCT [GA]G^G[AT]CC[CT]
RcaI 3109 TCATGA T^CATGA
SacII 903 CCGCGG CCGC^GG
SbfI 1265 CCTGCAGG CCTGCA^GG
SfuI 1104 TTCGAA TT^CGAA
SphI 1022 GCATGC GCATG^C
Sse8387I 1265 CCTGCAGG CCTGCA^GG
SspBI 2260 TGTACA T^GTACA
SspI 1533 AATATT AAT^ATT
Tsp45I 3660 GTCAC ^GT[GC]AC
Tth111I 81 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 3395 CCAAACATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AatII 1569 GACGTC GACGT^C
2616 GACGTC
AccI 1392 GTCTAC GT^[AC][GT]AC
3005 GTAGAC
AcyI 1569 GACGTC G[GA]^CG[CT]C
2616 GACGTC
AhdI 2598 GACAAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3100 GACCTGCTGTC
AocI 2905 CCTGAGG CC^T[ACGT]AGG
3037 CCTGAGG
Aor51HI 3408 AGCGCT AGC^GCT
3504 AGCGCT
AspEI 2598 GACAAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3100 GACCTGCTGTC
BalI 427 TGGCCA TGG^CCA
3117 TGGCCA
BglI 979 GCCCAGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2219 GCCTAGTCGGC
BlpI 945 GCTGAGC GC^T[ACGT]AGC
2339 GCTCAGC
Bpu1102I 945 GCTGAGC GC^T[ACGT]AGC
2339 GCTCAGC
BsaBI 1841 GATTGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2303 GATGAAGATC
BsaHI 1569 GACGTC G[GA]^CG[CT]C
2616 GACGTC
BsrBI 105 CCGCTC CCGCTC(-3/-3)
3545 CCGCTC
BsrFI 2362 ACCGGC [GA]^CCGG[CT]
2533 GCCGGC
Bsu36I 2905 CCTGAGG CC^T[ACGT]AGG
3037 CCTGAGG
CelII 945 GCTGAGC GC^T[ACGT]AGC
2339 GCTCAGC
Cfr10I 2362 ACCGGC [GA]^CCGG[CT]
2533 GCCGGC
DraIII 732 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG
1708 CACTGAGTG
Eam1105I 2598 GACAAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3100 GACCTGCTGTC
Eco47III 3408 AGCGCT AGC^GCT
3504 AGCGCT
Eco81I 2905 CCTGAGG CC^T[ACGT]AGG
3037 CCTGAGG
EcoRI 2179 GAATTC G^AATTC
3564 GAATTC
FauI 1153 CCCGC CCCGC(4/6)
905 GCGGG
Hin1I 1569 GACGTC G[GA]^CG[CT]C
2616 GACGTC
HindIII 420 AAGCTT A^AGCTT
2435 AAGCTT
MaeIII 3474 GTAAC ^GT[ACGT]AC
3660 GTCAC
MamI 1841 GATTGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2303 GATGAAGATC
MluNI 427 TGGCCA TGG^CCA
3117 TGGCCA
MscI 427 TGGCCA TGG^CCA
3117 TGGCCA
NspI 889 GCATGT [GA]CATG^[CT]
1022 GCATGC
PvuII 1287 CAGCTG CAG^CTG
1548 CAGCTG
SmaI 19 CCCGGG CCC^GGG
2038 CCCGGG
XmaCI 19 CCCGGG C^CCGGG
2038 CCCGGG
XmaI 19 CCCGGG C^CCGGG
2038 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 104 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApaI ApoI Asp700I AspHI AvaI
AvaII AvrII BanI BanII BbsI
BbvCI BbvI BcnI BfaI BlnI
BmyI BpmI BpuAI BsaI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I BspMI
BsrDI BsrI BssKI BstF5I BstNI
BstXI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MwoI NciI NcoI
NdeII NlaIII NlaIV PleI PstI
RsaI SacI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TaqI
TfiI Tru9I TseI Tsp509I TspRI
TthHB8I XcmI XhoII XmnI