##################################
# NO CUT ENZYMES ( 99 enzymes) #
##################################
AccIII AclI AhdI Alw44I Aor51HI
ApaLI AscI AspEI AspI AvaI
AviII BamHI BbeI BbrPI BbvCI
BcgI BclI BglII BlpI Bpu1102I
BsaAI BsaBI BsaI BseAI BsiEI
BsiWI BsmBI BsoBI BspDI BspEI
BspHI BsrBI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstYI
BstZ17I CelII ClaI CpoI DraIII
DrdI DsaI EagI Eam1105I EclXI
Eco47III Eco52I EcoO65I FbaI FseI
FspI KasI KspI MamI MflI
MluI MroI NarI NcoI NheI
NotI NruI NspV PacI PaeR7I
PflFI PmaCI PmeI PmlI PshAI
Psp1406I PstI PvuI RcaI RsrII
SacII SalI SbfI ScaI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI Sse8387I StuI SwaI Tth111I
XhoI XhoII XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AatII 3044 GACGTC GACGT^C
Acc65I 188 GGTACC G^GTACC
AccII 42 CGCG CG^CG
AcyI 3044 GACGTC G[GA]^CG[CT]C
AflII 2556 CTTAAG C^TTAAG
AgeI 185 ACCGGT A^CCGGT
AocI 294 CCTTAGG CC^T[ACGT]AGG
ApaI 2370 GGGCCC GGGCC^C
AseI 3162 ATTAAT AT^TAAT
AsnI 3162 ATTAAT AT^TAAT
Asp700I 1778 GAAATCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 188 GGTACC G^GTACC
AvrII 853 CCTAGG C^CTAGG
BalI 2609 TGGCCA TGG^CCA
BbsI 1179 GAAGAC GAAGAC(2/6)
BcnI 2 CCCGG CC^[GC]GG
BfrI 2556 CTTAAG C^TTAAG
BglI 1935 GCCTCAGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 853 CCTAGG C^CTAGG
BpuAI 1179 GAAGAC GAAGAC(2/6)
BsaHI 3044 GACGTC G[GA]^CG[CT]C
BsaWI 185 ACCGGT [AT]^CCGG[AT]
BspMI 1591 GCAGGT ACCTGC(4/8)
BsrDI 2786 GCAATG GCAATG(2/0)
BstUI 42 CGCG CG^CG
BstXI 1787 CCAAATTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 294 CCTTAGG CC^T[ACGT]AGG
EaeI 2609 TGGCCA [CT]^GGCC[GA]
Eco81I 294 CCTTAGG CC^T[ACGT]AGG
EcoNI 1999 CCTGGGACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 2039 GAATTC G^AATTC
FauI 40 CCCGC CCCGC(4/6)
HgaI 43 GCGTC GACGC(5/10)
Hin1I 3044 GACGTC G[GA]^CG[CT]C
HindIII 2472 AAGCTT A^AGCTT
KpnI 188 GGTACC GGTAC^C
MluNI 2609 TGGCCA TGG^CCA
MscI 2609 TGGCCA TGG^CCA
MvnI 42 CGCG CG^CG
NaeI 114 GCCGGC GCC^GGC
NciI 2 CCCGG CC^[GC]GG
NgoMIV 114 GCCGGC G^CCGGC
PinAI 185 ACCGGT A^CCGGT
PleI 1402 GAGTC GAGTC(4/5)
PpuMI 3636 AGGTCCC [GA]G^G[AT]CC[CT]
PshBI 3162 ATTAAT AT^TAAT
PvuII 58 CAGCTG CAG^CTG
SexAI 191 ACCTGGT A^CC[AT]GGT
SfcI 25 CTACAG C^T[GA][CT]AG
SphI 3392 GCATGC GCATG^C
XmnI 1778 GAAATCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccI 23 GTCTAC GT^[AC][GT]AC
2947 GTAGAC
AlwNI 1034 CAGAGGCTG CAG[ACGT][ACGT][ACGT]^CTG
1342 CAGCATCTG
BciVI 1899 GGATAC GTATCC(6/5)
408 GTATCC
BseRI 3273 CTCCTC GAGGAG(10/8)
100 GAGGAG
BsgI 357 CTGCAC GTGCAG(16/14)
2524 GTGCAG
BsmAI 353 GAGAC GTCTC(1/5)
3987 GTCTC
BsmI 1135 GCATTC GAATGC(1/-1)
1913 GCATTC
BsrFI 114 GCCGGC [GA]^CCGG[CT]
185 ACCGGT
CfoI 243 GCGC GCG^C
2755 GCGC
Cfr10I 114 GCCGGC [GA]^CCGG[CT]
185 ACCGGT
DraII 2370 GGGCCCC [GA]G^G[ACGT]CC[CT]
3636 AGGTCCC
EcoO109I 2370 GGGCCCC [GA]G^G[ACGT]CC[CT]
3636 AGGTCCC
EcoRV 334 GATATC GAT^ATC
3519 GATATC
HaeII 242 AGCGCC [GA]GCGC^[CT]
2754 AGCGCC
HaeIII 2371 GGCC GG^CC
2610 GGCC
HhaI 243 GCGC GCG^C
2755 GCGC
HinP1I 243 GCGC G^CGC
2755 GCGC
HpaI 1392 GTTAAC GTT^AAC
2517 GTTAAC
MfeI 1251 CAATTG C^AATTG
1307 CAATTG
MslI 171 CACAGGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
3608 CATAAGTGTG
MunI 1251 CAATTG C^AATTG
1307 CAATTG
NdeI 3906 CATATG CA^TATG
3914 CATATG
PflMI 1033 CCAGAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3204 CCACATATTGG
SacI 492 GAGCTC GAGCT^C
2453 GAGCTC
Van91I 1033 CCAGAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3204 CCACATATTGG
XcmI 513 CCAAATGCTAATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1551 CCAATGTAAACATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 82 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI ApoI AspHI AvaII BanI
BanII BbvI BfaI BmyI BpmI
BsaJI BsiHKAI BsiYI BslI BsmFI
Bsp1286I Bsp1407I BspLU11I BsrGI BsrI
BssKI BstF5I BstNI Cac8I Cfr13I
DdeI DpnII DraI EarI EcoRII
EcoT14I EcoT22I Fnu4HI FokI HapII
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MnlI MseI MspA1I
MspI MvaI MwoI NdeII NlaIII
NlaIV NsiI NspI RsaI SapI
Sau3AI Sau96I ScrFI SfaNI SmlI
SspBI SspI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XbaI