##################################
# NO CUT ENZYMES ( 89 enzymes) #
##################################
AatII Acc65I AclI AflII AgeI
AhdI Alw44I Aor51HI ApaLI AscI
Asp718I AspEI AviII AvrII BamHI
BfrI BlnI BlpI Bpu1102I BsaBI
BsiWI BsmBI Bsp1407I BspDI BspHI
BspLU11I BsrDI BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstXI
BstZ17I CelII ClaI CpoI DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoO65I EcoRI EcoRV EcoT22I FseI
FspI HincII HindII HindIII HpaI
KpnI KspI MamI MfeI MunI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PinAI PmeI
PshAI Psp1406I RcaI RsrII SacII
SalI SbfI ScaI SexAI SfiI
SfuI SmlI SnaBI SpeI Sse8387I
SspBI SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AcsI 679 AAATTC [GA]^AATT[CT]
AflIII 800 ACGCGT A^C[GA][CT]GT
ApoI 679 AAATTC [GA]^AATT[CT]
Asp700I 2548 GAAGTAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 1502 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 137 CCCGGG C^[CT]CG[GA]G
BalI 1131 TGGCCA TGG^CCA
BbrPI 788 CACGTG CAC^GTG
BcgI 2443 CGACTCTTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1471 GTATCC GTATCC(6/5)
BclI 524 TGATCA T^GATCA
BglI 2194 GCCCTCATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaI 1500 GAGACC GGTCTC(1/5)
BsoBI 137 CCCGGG C^[CT]CG[GA]G
BspMI 401 ACCTGC ACCTGC(4/8)
BstAPI 1955 GCAATTTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DraI 2629 TTTAAA TTT^AAA
DraIII 2348 CACATCGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 2306 CCATGG C^C[GA][CT]GG
EaeI 1131 TGGCCA [CT]^GGCC[GA]
EcoNI 2827 CCTTGGAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 524 TGATCA T^GATCA
MluI 800 ACGCGT A^CGCGT
MluNI 1131 TGGCCA TGG^CCA
MscI 1131 TGGCCA TGG^CCA
NaeI 1085 GCCGGC GCC^GGC
NcoI 2306 CCATGG C^CATGG
NgoMIV 1085 GCCGGC G^CCGGC
NspI 1199 GCATGC [GA]CATG^[CT]
PflFI 1502 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 788 CACGTG CAC^GTG
PmlI 788 CACGTG CAC^GTG
PpuMI 367 GGGTCCT [GA]G^G[AT]CC[CT]
PstI 2375 CTGCAG CTGCA^G
PvuI 26 CGATCG CGAT^CG
SgrAI 82 CACCGGCG C[GA]^CCGG[CT]G
SmaI 137 CCCGGG CCC^GGG
SphI 1199 GCATGC GCATG^C
SspI 262 AATATT AAT^ATT
StuI 283 AGGCCT AGG^CCT
Tth111I 1502 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 2306 CCATGGACACCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 137 CCCGGG C^CCGGG
XmaI 137 CCCGGG C^CCGGG
XmnI 2548 GAAGTAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccI 744 GTCTAC GT^[AC][GT]AC
1508 GTCTAC
AccIII 931 TCCGGA T^CCGGA
1187 TCCGGA
AlwI 651 GATCC GGATC(4/5)
795 GGATC
AocI 156 CCTCAGG CC^T[ACGT]AGG
286 CCTCAGG
ApaI 1737 GGGCCC GGGCC^C
1844 GGGCCC
AseI 2151 ATTAAT AT^TAAT
2609 ATTAAT
AsnI 2151 ATTAAT AT^TAAT
2609 ATTAAT
BbvCI 371 CCTCAGC CCTCAGC(-5/-2)
1906 GCTGAGG
BfaI 1971 CTAG C^TAG
2191 CTAG
BglII 541 AGATCT A^GATCT
1043 AGATCT
BsaAI 788 CACGTG [CT]AC^GT[GA]
1378 TACGTG
BseAI 931 TCCGGA T^CCGGA
1187 TCCGGA
BsiEI 26 CGATCG CG[GA][CT]^CG
170 CGACCG
BspEI 931 TCCGGA T^CCGGA
1187 TCCGGA
BsrBI 481 GAGCGG CCGCTC(-3/-3)
1143 GAGCGG
Bsu36I 156 CCTCAGG CC^T[ACGT]AGG
286 CCTCAGG
Eco81I 156 CCTCAGG CC^T[ACGT]AGG
286 CCTCAGG
MaeI 1971 CTAG C^TAG
2191 CTAG
MroI 931 TCCGGA T^CCGGA
1187 TCCGGA
MslI 1200 CATGCTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1422 CACCTGTGTG
PflMI 411 CCAGCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2002 CCAACAGCTGG
PshBI 2151 ATTAAT AT^TAAT
2609 ATTAAT
SacI 358 GAGCTC GAGCT^C
2079 GAGCTC
SapI 1018 GAAGAGC GCTCTTC(1/4)
1068 GAAGAGC
SfcI 2375 CTGCAG C^T[GA][CT]AG
2520 CTATAG
Van91I 411 CCAGCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2002 CCAACAGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 98 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwNI AspHI AvaII BanI BanII
BbeI BbsI BbvI BcnI BmyI
BpmI BpuAI BsaHI BsaJI BsaWI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI Bsp1286I BsrFI
BsrI BssKI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII EarI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI KasI Ksp632I MaeII MaeIII
MboI MboII MflI MnlI MseI
MspA1I MspI MvaI MvnI MwoI
NarI NciI NdeII NlaIII NlaIV
PleI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfoI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII