##################################
# NO CUT ENZYMES ( 92 enzymes) #
##################################
AatII Acc65I AccIII AgeI AhdI
Alw44I AocI ApaI ApaLI AscI
Asp718I AspEI AviII AvrII BamHI
BbeI BbvCI BcnI BglII BlnI
BlpI Bpu1102I BseAI BseRI BsiEI
BsiWI Bsp1407I BspEI BsrBI BsrGI
BssHII BssSI BstAPI BstBI BstEII
BstPI Bsu36I CelII CpoI EagI
Eam1105I EclXI Eco52I Eco81I EcoO65I
EcoT22I FseI FspI HincII HindII
HpaI KasI KpnI KspI MfeI
MluI MroI MunI NaeI NarI
NciI NgoMIV NotI NruI NsiI
NspV PacI PaeR7I PinAI PmeI
PvuI RsrII SacII SalI SapI
SbfI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SphI Sse8387I
SspBI StuI SwaI XbaI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AccI 4438 GTATAC GT^[AC][GT]AC
AccII 292 CGCG CG^CG
AclI 3386 AACGTT AA^CGTT
AcyI 1528 GGCGTC G[GA]^CG[CT]C
AvaI 2018 CTCGGG C^[CT]CG[GA]G
BalI 143 TGGCCA TGG^CCA
BanI 3973 GGCACC G^G[CT][GA]CC
BanII 20 GAGCTC G[GA]GC[CT]^C
BbrPI 1243 CACGTG CAC^GTG
BglI 3502 GCCTTTGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 1243 CACGTG [CT]AC^GT[GA]
BsaBI 3286 GATGCCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1528 GGCGTC G[GA]^CG[CT]C
BsaI 4241 GGTCTC GGTCTC(1/5)
BsmBI 1634 CGTCTC CGTCTC(1/5)
BsoBI 2018 CTCGGG C^[CT]CG[GA]G
BspDI 1852 ATCGAT AT^CGAT
BsrFI 3046 ACCGGC [GA]^CCGG[CT]
Bst1107I 4438 GTATAC GTA^TAC
BstUI 292 CGCG CG^CG
BstXI 782 CCATTGAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 3372 AGATCC [GA]^GATC[CT]
BstZ17I 4438 GTATAC GTA^TAC
Cfr10I 3046 ACCGGC [GA]^CCGG[CT]
ClaI 1852 ATCGAT AT^CGAT
DraIII 4122 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 3403 GACCAGAAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 143 TGGCCA [CT]^GGCC[GA]
EcoRV 1849 GATATC GAT^ATC
Hin1I 1528 GGCGTC G[GA]^CG[CT]C
MamI 3286 GATGCCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 3372 AGATCC [GA]^GATC[CT]
MluNI 143 TGGCCA TGG^CCA
MscI 143 TGGCCA TGG^CCA
MspA1I 1935 CAGCTG C[AC]G^C[GT]G
MvnI 292 CGCG CG^CG
NdeI 3415 CATATG CA^TATG
NheI 1446 GCTAGC G^CTAGC
PflMI 988 CCAAATAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1243 CACGTG CAC^GTG
PmlI 1243 CACGTG CAC^GTG
PshAI 476 GACGGTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 3386 AACGTT AA^CGTT
PvuII 1935 CAGCTG CAG^CTG
SacI 20 GAGCTC GAGCT^C
Van91I 988 CCAAATAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 782 CCATTGAGTTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 3372 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AflII 879 CTTAAG C^TTAAG
2542 CTTAAG
Aor51HI 569 AGCGCT AGC^GCT
3920 AGCGCT
AspHI 20 GAGCTC G[AT]GC[AT]^C
3990 GAGCAC
AspI 1518 GACTAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1958 GACATGGTC
BcgI 337 GCAGTGACTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1320 GCAGACACCTCG
BclI 1467 TGATCA T^GATCA
3123 TGATCA
BfrI 879 CTTAAG C^TTAAG
2542 CTTAAG
BsaWI 1863 ACCGGA [AT]^CCGG[AT]
3482 TCCGGT
BsgI 248 GTGCAG GTGCAG(16/14)
3690 GTGCAG
BsiHKAI 20 GAGCTC G[AT]GC[AT]^C
3990 GAGCAC
BsmI 2400 GAATGC GAATGC(1/-1)
4204 GAATGC
CfoI 570 GCGC GCG^C
3921 GCGC
DsaI 158 CCATGG C^C[GA][CT]GG
4279 CCATGG
Eco47III 569 AGCGCT AGC^GCT
3920 AGCGCT
EcoRI 1158 GAATTC G^AATTC
3881 GAATTC
FauI 55 CCCGC CCCGC(4/6)
293 GCGGG
FbaI 1467 TGATCA T^GATCA
3123 TGATCA
HaeII 569 AGCGCT [GA]GCGC^[CT]
3920 AGCGCT
HhaI 570 GCGC GCG^C
3921 GCGC
HinP1I 570 GCGC G^CGC
3921 GCGC
HindIII 2167 AAGCTT A^AGCTT
3202 AAGCTT
NcoI 158 CCATGG C^CATGG
4279 CCATGG
PflFI 1518 GACTAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1958 GACATGGTC
PpuMI 2701 AGGTCCT [GA]G^G[AT]CC[CT]
4250 GGGACCC
ScaI 2098 AGTACT AGT^ACT
2761 AGTACT
Tth111I 1518 GACTAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1958 GACATGGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 92 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI ApoI AseI AsnI
Asp700I AvaII BbsI BbvI BciVI
BfaI BmyI BpmI BpuAI BsaJI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspHI BspLU11I BspMI BsrDI BsrI
BssKI BstF5I BstNI Cac8I Cfr13I
DdeI DpnII DraI DraII EarI
EcoNI EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HgaI HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MnlI
MseI MslI MspI MvaI MwoI
NdeII NlaIII NlaIV NspI PleI
PshBI PstI RcaI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SpeI SspI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XmnI