##################################
# NO CUT ENZYMES ( 83 enzymes) #
##################################
AatII Acc65I AccIII AclI AgeI
AhdI Alw44I Aor51HI ApaI ApaLI
AscI Asp700I Asp718I AspEI AspI
AviII BamHI BbsI BglI BpuAI
BsaBI BsaWI BseAI BsiEI BsiWI
BspDI BspEI BspLU11I BsrFI BssHII
BssSI BstEII BstPI Cfr10I ClaI
CpoI EagI Eam1105I EclXI Eco47III
Eco52I EcoO65I EcoRV FseI FspI
HincII HindII HpaI KpnI KspI
MamI MfeI MluI MroI MunI
NaeI NgoMIV NheI NotI NruI
PaeR7I PflFI PinAI PmeI Psp1406I
PvuI RsrII SacII SalI SapI
SbfI SexAI SfiI SgrAI SmaI
SnaBI Sse8387I SwaI Tth111I XhoI
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AlwNI 1612 CAGCCACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 3145 CCTTAGG CC^T[ACGT]AGG
AseI 3676 ATTAAT AT^TAAT
AsnI 3676 ATTAAT AT^TAAT
AvaI 213 CTCGGG C^[CT]CG[GA]G
BbeI 2031 GGCGCC GGCGC^C
BclI 668 TGATCA T^GATCA
BcnI 2699 CCGGG CC^[GC]GG
BglII 4152 AGATCT A^GATCT
BlpI 2236 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2236 GCTCAGC GC^T[ACGT]AGC
BsmBI 3920 CGTCTC CGTCTC(1/5)
BsmI 1820 GCATTC GAATGC(1/-1)
BsoBI 213 CTCGGG C^[CT]CG[GA]G
Bsp1407I 3349 TGTACA T^GTACA
BspHI 2741 TCATGA T^CATGA
BsrBI 503 CCGCTC CCGCTC(-3/-3)
BsrGI 3349 TGTACA T^GTACA
BstBI 2440 TTCGAA TT^CGAA
BstXI 3037 CCAAAATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 3145 CCTTAGG CC^T[ACGT]AGG
CelII 2236 GCTCAGC GC^T[ACGT]AGC
DrdI 3009 GACCTTGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 3145 CCTTAGG CC^T[ACGT]AGG
EcoNI 260 CCTTGCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 3999 GAATTC G^AATTC
EcoT22I 3055 ATGCAT ATGCA^T
FbaI 668 TGATCA T^GATCA
HaeII 2031 GGCGCC [GA]GCGC^[CT]
HapII 2699 CCGG C^CGG
HpaII 2699 CCGG C^CGG
KasI 2031 GGCGCC G^GCGCC
MspI 2699 CCGG C^CGG
NarI 2031 GGCGCC GG^CGCC
NciI 2699 CCGGG CC^[GC]GG
NcoI 510 CCATGG C^CATGG
NsiI 3055 ATGCAT ATGCA^T
NspV 2440 TTCGAA TT^CGAA
PacI 3854 TTAATTAA TTAAT^TAA
PpuMI 2351 GGGTCCT [GA]G^G[AT]CC[CT]
PshAI 426 GACCAAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 3676 ATTAAT AT^TAAT
RcaI 2741 TCATGA T^CATGA
ScaI 487 AGTACT AGT^ACT
SfoI 2031 GGCGCC GGC^GCC
SfuI 2440 TTCGAA TT^CGAA
SpeI 2779 ACTAGT A^CTAGT
SphI 4134 GCATGC GCATG^C
SspBI 3349 TGTACA T^GTACA
XbaI 2634 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AccII 2384 CGCG CG^CG
3067 CGCG
AflII 2372 CTTAAG C^TTAAG
3762 CTTAAG
AvrII 1089 CCTAGG C^CTAGG
1728 CCTAGG
BalI 530 TGGCCA TGG^CCA
2985 TGGCCA
BbvCI 802 CCTCAGC CCTCAGC(-5/-2)
2894 CCTCAGC
BcgI 2883 CGATTCTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
464 GCAGACACATCG
BciVI 351 GGATAC GTATCC(6/5)
2360 GTATCC
BfrI 2372 CTTAAG C^TTAAG
3762 CTTAAG
BlnI 1089 CCTAGG C^CTAGG
1728 CCTAGG
BsaI 207 GAGACC GGTCTC(1/5)
384 GGTCTC
BsgI 2664 CTGCAC GTGCAG(16/14)
2832 GTGCAG
BspMI 2052 ACCTGC ACCTGC(4/8)
2771 GCAGGT
BsrDI 59 CATTGC GCAATG(2/0)
4162 CATTGC
Bst1107I 1053 GTATAC GTA^TAC
1284 GTATAC
BstAPI 2728 GCAGATTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2858 GCAACCTCTGC
BstUI 2384 CGCG CG^CG
3067 CGCG
BstZ17I 1053 GTATAC GTA^TAC
1284 GTATAC
CfoI 2032 GCGC GCG^C
3068 GCGC
DraII 2038 AGGCCCC [GA]G^G[ACGT]CC[CT]
2351 GGGTCCT
DsaI 510 CCATGG C^C[GA][CT]GG
1621 CCACGG
EcoO109I 2038 AGGCCCC [GA]G^G[ACGT]CC[CT]
2351 GGGTCCT
HhaI 2032 GCGC GCG^C
3068 GCGC
HinP1I 2032 GCGC G^CGC
3068 GCGC
HindIII 239 AAGCTT A^AGCTT
2447 AAGCTT
MluNI 530 TGGCCA TGG^CCA
2985 TGGCCA
MscI 530 TGGCCA TGG^CCA
2985 TGGCCA
MvnI 2384 CGCG CG^CG
3067 CGCG
NdeI 3486 CATATG CA^TATG
3538 CATATG
PstI 3818 CTGCAG CTGCA^G
4009 CTGCAG
SacI 1226 GAGCTC GAGCT^C
1237 GAGCTC
StuI 2607 AGGCCT AGG^CCT
3822 AGGCCT
TaqI 2441 TCGA T^CGA
3414 TCGA
TthHB8I 2441 TCGA T^CGA
3414 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 92 enzymes) #
#########################################
AccI AciI AcsI AcyI AfaI
AflIII AluI AlwI ApoI AspHI
AvaII BanI BanII BbrPI BbvI
BfaI BmyI BpmI BsaAI BsaHI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrI BssKI
BstF5I BstNI BstYI Cac8I Cfr13I
DdeI DpnII DraI DraIII EaeI
EarI EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HgaI Hin1I HinfI
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MvaI MwoI
NdeII NlaIII NlaIV NspI PflMI
PleI PmaCI PmlI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspI StyI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI Van91I
XcmI XhoII