##################################
# NO CUT ENZYMES ( 82 enzymes) #
##################################
AccIII AclI AgeI AhdI AscI
AseI AsnI AspEI AspI BbeI
BbrPI BcgI BsaAI BsaBI BsaWI
BseAI BsiEI BsiWI BsmBI BspDI
BspEI BsrFI BssHII Bst1107I BstAPI
BstBI BstZ17I Cfr10I ClaI CpoI
DraI DraIII DrdI EagI Eam1105I
EclXI Eco52I EcoRI EcoRV EcoT22I
FseI HindIII KasI KspI MamI
MfeI MluI MroI MunI NaeI
NarI NdeI NgoMIV NheI NotI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshBI
Psp1406I PvuI RsrII SacII SalI
SapI SexAI SfiI SfoI SfuI
SgrAI SnaBI SpeI SwaI Tth111I
XbaI XhoI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AatII 1085 GACGTC GACGT^C
Acc65I 1986 GGTACC G^GTACC
AccI 1397 GTAGAC GT^[AC][GT]AC
AcsI 2999 AAATTC [GA]^AATT[CT]
AflII 3572 CTTAAG C^TTAAG
Alw44I 1733 GTGCAC G^TGCAC
ApaLI 1733 GTGCAC G^TGCAC
ApoI 2999 AAATTC [GA]^AATT[CT]
Asp700I 2159 GAAGGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1986 GGTACC G^GTACC
AvrII 548 CCTAGG C^CTAGG
BamHI 771 GGATCC G^GATCC
BanI 1986 GGTACC G^G[CT][GA]CC
BciVI 2394 GTATCC GTATCC(6/5)
BfrI 3572 CTTAAG C^TTAAG
BglI 1553 GCCTGGTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1878 AGATCT A^GATCT
BlnI 548 CCTAGG C^CTAGG
BsgI 2189 GTGCAG GTGCAG(16/14)
BsmI 2163 GCATTC GAATGC(1/-1)
Bsp1407I 864 TGTACA T^GTACA
BspHI 2172 TCATGA T^CATGA
BspLU11I 1566 ACATGT A^CATGT
BsrBI 2666 GAGCGG CCGCTC(-3/-3)
BsrDI 3231 GCAATG GCAATG(2/0)
BsrGI 864 TGTACA T^GTACA
BssSI 1436 CACGAG CACGAG(-5/-1)
HincII 415 GTTAAC GT[CT]^[GA]AC
HindII 415 GTTAAC GT[CT]^[GA]AC
HpaI 415 GTTAAC GTT^AAC
KpnI 1986 GGTACC GGTAC^C
NruI 922 TCGCGA TCG^CGA
RcaI 2172 TCATGA T^CATGA
SacI 2525 GAGCTC GAGCT^C
SbfI 1021 CCTGCAGG CCTGCA^GG
ScaI 2232 AGTACT AGT^ACT
SmaI 1473 CCCGGG CCC^GGG
SphI 781 GCATGC GCATG^C
Sse8387I 1021 CCTGCAGG CCTGCA^GG
SspBI 864 TGTACA T^GTACA
SspI 3729 AATATT AAT^ATT
StuI 163 AGGCCT AGG^CCT
XmaCI 1473 CCCGGG C^CCGGG
XmaI 1473 CCCGGG C^CCGGG
XmnI 2159 GAAGGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AccII 24 CGCG CG^CG
923 CGCG
AcyI 43 GGCGTC G[GA]^CG[CT]C
1085 GACGTC
AviII 2781 TGCGCA TGC^GCA
3525 TGCGCA
BsaHI 43 GGCGTC G[GA]^CG[CT]C
1085 GACGTC
BstUI 24 CGCG CG^CG
923 CGCG
EarI 1169 CTCTTC CTCTTC(1/4)
122 GAAGAG
FauI 1344 CCCGC CCCGC(4/6)
2668 GCGGG
FspI 2781 TGCGCA TGC^GCA
3525 TGCGCA
HgaI 44 GCGTC GACGC(5/10)
2584 GCGTC
Hin1I 43 GGCGTC G[GA]^CG[CT]C
1085 GACGTC
Ksp632I 1169 CTCTTC CTCTTC(1/4)
122 GAAGAG
MvnI 24 CGCG CG^CG
923 CGCG
PflMI 2959 CCAACATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3208 CCACTTCTTGG
PpuMI 430 GGGACCC [GA]G^G[AT]CC[CT]
1320 GGGACCC
PshAI 485 GACCGAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1322 GACCCCAGTC
PstI 951 CTGCAG CTGCA^G
1022 CTGCAG
TaqI 2431 TCGA T^CGA
2587 TCGA
TthHB8I 2431 TCGA T^CGA
2587 TCGA
Van91I 2959 CCAACATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3208 CCACTTCTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 112 enzymes) #
#########################################
AciI AfaI AflIII AluI AlwI
AlwNI AocI Aor51HI ApaI AspHI
AvaI AvaII BalI BanII BbsI
BbvCI BbvI BclI BcnI BfaI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrI BssKI BstEII BstF5I
BstNI BstPI BstXI BstYI Bsu36I
Cac8I CelII CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI Eco47III
Eco81I EcoNI EcoO109I EcoO65I EcoRII
EcoT14I FbaI Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PleI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
XcmI XhoII