##################################
# NO CUT ENZYMES ( 87 enzymes) #
##################################
AatII Acc65I AccIII AclI AgeI
AhdI Alw44I ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspHI
AviII BalI BbvCI BcgI BciVI
BglI BlpI Bpu1102I BsaBI BsaWI
BseAI BsiHKAI BsiWI BspDI BspEI
BsrBI BssHII BssSI BstAPI CelII
ClaI CpoI DrdI Eam1105I EcoRI
EcoRV EcoT22I FseI FspI HgaI
HincII HindII HpaI KpnI KspI
MamI MfeI MluI MluNI MroI
MscI MunI NotI NruI NsiI
PacI PinAI PmeI PshAI PshBI
Psp1406I PvuI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SwaI XbaI XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AccII 57 CGCG CG^CG
AflII 794 CTTAAG C^TTAAG
Aor51HI 523 AGCGCT AGC^GCT
ApaI 559 GGGCCC GGGCC^C
BamHI 3748 GGATCC G^GATCC
BbrPI 1295 CACGTG CAC^GTG
BfrI 794 CTTAAG C^TTAAG
BpmI 2670 CTGGAG CTGGAG(16/14)
BsiEI 861 CGGCCG CG[GA][CT]^CG
BsmBI 3800 GAGACG CGTCTC(1/5)
BsmI 3407 GCATTC GAATGC(1/-1)
BspHI 3256 TCATGA T^CATGA
BsrDI 640 CATTGC GCAATG(2/0)
Bst1107I 3185 GTATAC GTA^TAC
BstBI 744 TTCGAA TT^CGAA
BstUI 57 CGCG CG^CG
BstXI 645 CCAATGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 3185 GTATAC GTA^TAC
DraIII 2084 CACCTAGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 861 CGGCCG C^GGCCG
EclXI 861 CGGCCG C^GGCCG
Eco47III 523 AGCGCT AGC^GCT
Eco52I 861 CGGCCG C^GGCCG
MvnI 57 CGCG CG^CG
NaeI 137 GCCGGC GCC^GGC
NgoMIV 137 GCCGGC G^CCGGC
NheI 3940 GCTAGC G^CTAGC
NspV 744 TTCGAA TT^CGAA
PaeR7I 3435 CTCGAG C^TCGAG
PflMI 1305 CCAGGACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1295 CACGTG CAC^GTG
PmlI 1295 CACGTG CAC^GTG
RcaI 3256 TCATGA T^CATGA
SfuI 744 TTCGAA TT^CGAA
SspI 1569 AATATT AAT^ATT
Van91I 1305 CCAGGACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 3435 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 37 enzymes) #
###################################
AcyI 38 GGCGCC G[GA]^CG[CT]C
190 GGCGCC
AocI 2208 CCTGAGG CC^T[ACGT]AGG
2708 CCTTAGG
AspI 3489 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3802 GACGGAGTC
AvrII 2475 CCTAGG C^CTAGG
3795 CCTAGG
BanII 214 GGGCTC G[GA]GC[CT]^C
559 GGGCCC
BbeI 38 GGCGCC GGCGC^C
190 GGCGCC
BbsI 1030 GAAGAC GAAGAC(2/6)
1209 GTCTTC
BclI 969 TGATCA T^GATCA
1277 TGATCA
BglII 1167 AGATCT A^GATCT
2239 AGATCT
BlnI 2475 CCTAGG C^CTAGG
3795 CCTAGG
BpuAI 1030 GAAGAC GAAGAC(2/6)
1209 GTCTTC
BsaAI 1295 CACGTG [CT]AC^GT[GA]
2983 TACGTG
BsaHI 38 GGCGCC G[GA]^CG[CT]C
190 GGCGCC
BsaI 674 GAGACC GGTCTC(1/5)
2336 GGTCTC
BspLU11I 1439 ACATGT A^CATGT
3955 ACATGT
BspMI 447 ACCTGC ACCTGC(4/8)
2348 GCAGGT
BsrFI 137 GCCGGC [GA]^CCGG[CT]
3895 GCCGGT
BstEII 2081 GGTCACC G^GT[ACGT]ACC
2703 GGTGACC
BstPI 2081 GGTCACC G^GT[ACGT]ACC
2703 GGTGACC
Bsu36I 2208 CCTGAGG CC^T[ACGT]AGG
2708 CCTTAGG
Cfr10I 137 GCCGGC [GA]^CCGG[CT]
3895 GCCGGT
DsaI 180 CCATGG C^C[GA][CT]GG
3858 CCATGG
Eco81I 2208 CCTGAGG CC^T[ACGT]AGG
2708 CCTTAGG
EcoNI 538 CCTCATCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2564 CCTCTTGAAGG
EcoO65I 2081 GGTCACC G^GT[ACGT]ACC
2703 GGTGACC
FbaI 969 TGATCA T^GATCA
1277 TGATCA
Hin1I 38 GGCGCC G[GA]^CG[CT]C
190 GGCGCC
HindIII 2824 AAGCTT A^AGCTT
4004 AAGCTT
KasI 38 GGCGCC G^GCGCC
190 GGCGCC
NarI 38 GGCGCC GG^CGCC
190 GGCGCC
NcoI 180 CCATGG C^CATGG
3858 CCATGG
NdeI 1587 CATATG CA^TATG
2998 CATATG
PflFI 3489 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3802 GACGGAGTC
PpuMI 7 GGGTCCT [GA]G^G[AT]CC[CT]
2358 AGGTCCC
SfoI 38 GGCGCC GGC^GCC
190 GGCGCC
StuI 1011 AGGCCT AGG^CCT
2801 AGGCCT
Tth111I 3489 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3802 GACGGAGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 97 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApoI AvaI
AvaII BanI BbvI BcnI BfaI
BmyI BsaJI BseRI BsgI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
Bsp1407I BsrGI BsrI BssKI BstF5I
BstNI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraI DraII EaeI
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
PleI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspBI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XcmI XhoII