##################################
# NO CUT ENZYMES ( 73 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AgeI Aor51HI AscI Asp718I AspI
AviII AvrII BbrPI BcgI BciVI
BfrI BlnI BsaBI BsaWI BseAI
BsiWI BspDI BspEI BspHI BsrFI
BssHII Bst1107I BstEII BstPI BstZ17I
Cfr10I ClaI CpoI DraIII DrdI
Eco47III EcoO65I EcoT22I FseI FspI
HpaI KpnI MamI MfeI MluI
MroI MunI NaeI NgoMIV NheI
NruI NsiI PacI PflFI PinAI
PmaCI PmeI PmlI PshAI Psp1406I
PvuI RcaI RsrII SalI SapI
SexAI SfiI SgrAI SnaBI SpeI
SphI SspI Tth111I
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
Alw44I 1752 GTGCAC G^TGCAC
ApaLI 1752 GTGCAC G^TGCAC
BbeI 221 GGCGCC GGCGC^C
BbvCI 3496 GCTGAGG CCTCAGC(-5/-2)
BglII 212 AGATCT A^GATCT
BsaI 4934 GGTCTC GGTCTC(1/5)
BsiEI 53 CGGCCG CG[GA][CT]^CG
BspLU11I 3883 ACATGT A^CATGT
BsrDI 3687 CATTGC GCAATG(2/0)
BssSI 1129 CTCGTG CACGAG(-5/-1)
BstAPI 1866 GCAGTCCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 2051 TTCGAA TT^CGAA
EagI 53 CGGCCG C^GGCCG
EclXI 53 CGGCCG C^GGCCG
Eco52I 53 CGGCCG C^GGCCG
EcoNI 2610 CCTTCCTAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 2042 GATATC GAT^ATC
FauI 2633 CCCGC CCCGC(4/6)
HincII 229 GTTGAC GT[CT]^[GA]AC
HindII 229 GTTGAC GT[CT]^[GA]AC
KasI 221 GGCGCC G^GCGCC
KspI 56 CCGCGG CCGC^GG
NarI 221 GGCGCC GG^CGCC
NotI 52 GCGGCCGC GC^GGCCGC
NspV 2051 TTCGAA TT^CGAA
PaeR7I 2828 CTCGAG C^TCGAG
SacII 56 CCGCGG CCGC^GG
SbfI 402 CCTGCAGG CCTGCA^GG
ScaI 2375 AGTACT AGT^ACT
SfoI 221 GGCGCC GGC^GCC
SfuI 2051 TTCGAA TT^CGAA
Sse8387I 402 CCTGCAGG CCTGCA^GG
StuI 3324 AGGCCT AGG^CCT
SwaI 5057 ATTTAAAT ATTT^AAAT
XbaI 3373 TCTAGA T^CTAGA
XcmI 2522 CCATCAGTGCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 2828 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 38 enzymes) #
###################################
AccII 57 CGCG CG^CG
2241 CGCG
AflIII 2879 ACGTGT A^C[GA][CT]GT
3883 ACATGT
AhdI 1771 GACAAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4449 GACTGAATGTC
AlwNI 658 CAGCACCTG CAG[ACGT][ACGT][ACGT]^CTG
4659 CAGAATCTG
AocI 1616 CCTCAGG CC^T[ACGT]AGG
2614 CCTAAGG
AseI 4424 ATTAAT AT^TAAT
5246 ATTAAT
AsnI 4424 ATTAAT AT^TAAT
5246 ATTAAT
Asp700I 1784 GAATGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3377 GAAATCTTTC
AspEI 1771 GACAAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4449 GACTGAATGTC
BclI 287 TGATCA T^GATCA
2924 TGATCA
BglI 1197 GCCTGAGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2530 GCCATGGAGGC
BlpI 3081 GCTCAGC GC^T[ACGT]AGC
4876 GCTCAGC
Bpu1102I 3081 GCTCAGC GC^T[ACGT]AGC
4876 GCTCAGC
BsaAI 2878 TACGTG [CT]AC^GT[GA]
5281 CACGTA
Bsp1407I 2697 TGTACA T^GTACA
4118 TGTACA
BsrBI 1185 GAGCGG CCGCTC(-3/-3)
1201 GAGCGG
BsrGI 2697 TGTACA T^GTACA
4118 TGTACA
BstUI 57 CGCG CG^CG
2241 CGCG
Bsu36I 1616 CCTCAGG CC^T[ACGT]AGG
2614 CCTAAGG
CelII 3081 GCTCAGC GC^T[ACGT]AGC
4876 GCTCAGC
Eam1105I 1771 GACAAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4449 GACTGAATGTC
Eco81I 1616 CCTCAGG CC^T[ACGT]AGG
2614 CCTAAGG
FbaI 287 TGATCA T^GATCA
2924 TGATCA
HaeII 221 GGCGCC [GA]GCGC^[CT]
1853 AGCGCC
HindIII 1624 AAGCTT A^AGCTT
3947 AAGCTT
MslI 4160 CACTCGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
4604 CATACATATG
MvnI 57 CGCG CG^CG
2241 CGCG
NcoI 2531 CCATGG C^CATGG
5270 CCATGG
NdeI 4370 CATATG CA^TATG
4608 CATATG
NspI 3883 ACATGT [GA]CATG^[CT]
4862 GCATGT
PpuMI 821 GGGTCCT [GA]G^G[AT]CC[CT]
2489 AGGTCCT
PshBI 4424 ATTAAT AT^TAAT
5246 ATTAAT
SacI 717 GAGCTC GAGCT^C
2419 GAGCTC
SmaI 818 CCCGGG CCC^GGG
857 CCCGGG
SspBI 2697 TGTACA T^GTACA
4118 TGTACA
XmaCI 818 CCCGGG C^CCGGG
857 CCCGGG
XmaI 818 CCCGGG C^CCGGG
857 CCCGGG
XmnI 1784 GAATGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3377 GAAATCTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 110 enzymes) #
#########################################
AccI AciI AcsI AcyI AfaI
AluI AlwI ApaI ApoI AspHI
AvaI AvaII BalI BamHI BanI
BanII BbsI BbvI BcnI BfaI
BmyI BpmI BpuAI BsaHI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsmI BsoBI
Bsp1286I BspMI BsrI BssKI BstF5I
BstNI BstXI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraI DraII
DsaI EaeI EarI EcoO109I EcoRI
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PflMI PleI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XhoII