##################################
# NO CUT ENZYMES ( 119 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AgeI AhdI AlwNI AocI
Aor51HI ApaI AscI Asp700I Asp718I
AspEI AspI AviII AvrII BalI
BbeI BbrPI BbsI BbvCI BcgI
BciVI BcnI BglI BglII BlnI
BpuAI BsaAI BsaWI BseAI BsiEI
BsiWI BsmBI BspDI BspEI BsrBI
BsrFI BssHII Bst1107I BstEII BstPI
BstUI BstXI BstZ17I Bsu36I CfoI
Cfr10I ClaI CpoI DrdI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRV EcoT14I
FauI FseI FspI HaeII HapII
HhaI HinP1I HpaII KasI KpnI
KspI MaeII MluI MluNI MroI
MscI MspI MvnI NaeI NarI
NciI NcoI NgoMIV NheI NotI
NruI PaeR7I PflFI PinAI PmaCI
PmeI PmlI Psp1406I PvuI RsrII
SacI SacII SalI SapI SbfI
SfiI SfoI SgrAI SmaI SnaBI
Sse8387I StuI StyI Tth111I XcmI
XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AcyI 62 GGCGTC G[GA]^CG[CT]C
Alw44I 2044 GTGCAC G^TGCAC
ApaLI 2044 GTGCAC G^TGCAC
AvaI 1687 CCCGAG C^[CT]CG[GA]G
BamHI 2659 GGATCC G^GATCC
BanI 1900 GGTGCC G^G[CT][GA]CC
BlpI 108 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 108 GCTGAGC GC^T[ACGT]AGC
BsaBI 2371 GATATAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 62 GGCGTC G[GA]^CG[CT]C
BsgI 1738 CTGCAC GTGCAG(16/14)
BsmFI 1347 GGGAC GGGAC(10/14)
BsmI 143 GCATTC GAATGC(1/-1)
BsoBI 1687 CCCGAG C^[CT]CG[GA]G
BspMI 1587 GCAGGT ACCTGC(4/8)
BsrDI 940 GCAATG GCAATG(2/0)
BssSI 988 CACGAG CACGAG(-5/-1)
BstBI 1228 TTCGAA TT^CGAA
CelII 108 GCTGAGC GC^T[ACGT]AGC
DraIII 988 CACGAGGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 880 CCGTGG C^C[GA][CT]GG
EarI 2547 GAAGAG CTCTTC(1/4)
EcoT22I 2511 ATGCAT ATGCA^T
HaeIII 662 GGCC GG^CC
Hin1I 62 GGCGTC G[GA]^CG[CT]C
HindIII 931 AAGCTT A^AGCTT
HpaI 2405 GTTAAC GTT^AAC
Ksp632I 2547 GAAGAG CTCTTC(1/4)
MamI 2371 GATATAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NdeI 157 CATATG CA^TATG
NsiI 2511 ATGCAT ATGCA^T
NspV 1228 TTCGAA TT^CGAA
PacI 1577 TTAATTAA TTAAT^TAA
PflMI 875 CCATGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1598 AGGACCC [GA]G^G[AT]CC[CT]
PshAI 536 GACATCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 457 CTGCAG CTGCA^G
SexAI 1485 ACCAGGT A^CC[AT]GGT
SfaNI 908 GCATC GCATC(5/9)
SfuI 1228 TTCGAA TT^CGAA
SpeI 2558 ACTAGT A^CTAGT
SwaI 1657 ATTTAAAT ATTT^AAAT
Van91I 875 CCATGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AflII 270 CTTAAG C^TTAAG
2664 CTTAAG
AflIII 593 ACATGT A^C[GA][CT]GT
2401 ACATGT
AspHI 2044 GTGCAC G[AT]GC[AT]^C
2803 GTGCTC
AvaII 1348 GGACC G^G[AT]CC
1599 GGACC
BanII 2598 GAGCCC G[GA]GC[CT]^C
2772 GAGCCC
BfrI 270 CTTAAG C^TTAAG
2664 CTTAAG
BpmI 1248 CTGGAG CTGGAG(16/14)
1384 CTGGAG
BseRI 1028 CTCCTC GAGGAG(10/8)
1047 GAGGAG
BsiHKAI 2044 GTGCAC G[AT]GC[AT]^C
2803 GTGCTC
Bsp1407I 2325 TGTACA T^GTACA
2350 TGTACA
BspHI 2249 TCATGA T^CATGA
2621 TCATGA
BspLU11I 593 ACATGT A^CATGT
2401 ACATGT
BsrGI 2325 TGTACA T^GTACA
2350 TGTACA
BstAPI 1854 GCAGTGCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1894 GCATGAGGTGC
BstYI 620 GGATCT [GA]^GATC[CT]
2659 GGATCC
DraII 661 AGGCCCT [GA]G^G[ACGT]CC[CT]
1598 AGGACCC
EcoO109I 661 AGGCCCT [GA]G^G[ACGT]CC[CT]
1598 AGGACCC
EcoRI 401 GAATTC G^AATTC
1439 GAATTC
HincII 2405 GTTAAC GT[CT]^[GA]AC
2831 GTCAAC
HindII 2405 GTTAAC GT[CT]^[GA]AC
2831 GTCAAC
HphI 2810 GGTGA GGTGA(8/7)
1106 TCACC
MfeI 355 CAATTG C^AATTG
489 CAATTG
MflI 620 GGATCT [GA]^GATC[CT]
2659 GGATCC
MslI 1656 CATTTAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2574 CACACTCATG
MspA1I 745 CAGCTG C[AC]G^C[GT]G
1245 CAGCTG
MunI 355 CAATTG C^AATTG
489 CAATTG
PvuII 745 CAGCTG CAG^CTG
1245 CAGCTG
RcaI 2249 TCATGA T^CATGA
2621 TCATGA
ScaI 371 AGTACT AGT^ACT
2561 AGTACT
SphI 518 GCATGC GCATG^C
730 GCATGC
SspBI 2325 TGTACA T^GTACA
2350 TGTACA
SspI 2307 AATATT AAT^ATT
2607 AATATT
XbaI 345 TCTAGA T^CTAGA
764 TCTAGA
XhoII 620 GGATCT [GA]^GATC[CT]
2659 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 62 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AseI AsnI BbvI BclI
BfaI BmyI BsaI BsaJI BsiYI
BslI BsmAI Bsp1286I BsrI BssKI
BstF5I BstNI Cac8I Cfr13I DdeI
DpnII DraI EcoRII FbaI Fnu4HI
FokI HgaI HinfI ItaI MaeI
MaeIII MboI MboII MnlI MseI
MvaI MwoI NdeII NlaIII NlaIV
NspI PleI PshBI RsaI Sau3AI
Sau96I ScrFI SfcI SmlI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I