##################################
# NO CUT ENZYMES ( 95 enzymes) #
##################################
AatII Acc65I AccIII AcyI AflII
AflIII AgeI AhdI Aor51HI ApaI
AscI Asp718I AspEI AspI AvaI
AviII AvrII BanI BbeI BbrPI
BcgI BfrI BglII BlnI BlpI
Bpu1102I BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BseRI BsiWI BsoBI
BspEI BspHI BspLU11I BsrBI BssHII
BssSI BstEII BstPI CelII CpoI
DrdI Eam1105I Eco47III EcoNI EcoO65I
EcoRI FseI FspI Hin1I HpaI
KasI KpnI KspI MamI MluI
MroI NarI NheI NotI NruI
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PvuI RcaI
RsrII SacI SacII SalI SbfI
ScaI SfiI SfoI SgrAI SmaI
SnaBI SpeI Sse8387I StuI SwaI
Tth111I XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 77 enzymes) #
###################################
AccII 42 CGCG CG^CG
AclI 2157 AACGTT AA^CGTT
Alw44I 1155 GTGCAC G^TGCAC
AlwNI 1116 CAGACACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 360 CCTTAGG CC^T[ACGT]AGG
ApaLI 1155 GTGCAC G^TGCAC
AseI 242 ATTAAT AT^TAAT
AsnI 242 ATTAAT AT^TAAT
Asp700I 2096 GAAAGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII 715 GGACC G^G[AT]CC
BalI 1043 TGGCCA TGG^CCA
BamHI 1696 GGATCC G^GATCC
BanII 118 GGGCTC G[GA]GC[CT]^C
BbvCI 2288 CCTCAGC CCTCAGC(-5/-2)
BciVI 2339 GTATCC GTATCC(6/5)
BclI 352 TGATCA T^GATCA
BcnI 1140 CCCGG CC^[GC]GG
BglI 307 GCCAACAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsgI 1407 CTGCAC GTGCAG(16/14)
BsiEI 30 CGGCCG CG[GA][CT]^CG
BsmI 2610 GAATGC GAATGC(1/-1)
Bsp1407I 532 TGTACA T^GTACA
BspDI 267 ATCGAT AT^CGAT
BspMI 260 GCAGGT ACCTGC(4/8)
BsrFI 485 GCCGGC [GA]^CCGG[CT]
BsrGI 532 TGTACA T^GTACA
BstAPI 542 GCATGTATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 888 TTCGAA TT^CGAA
BstUI 42 CGCG CG^CG
BstYI 1696 GGATCC [GA]^GATC[CT]
Bsu36I 360 CCTTAGG CC^T[ACGT]AGG
CfoI 2 GCGC GCG^C
Cfr10I 485 GCCGGC [GA]^CCGG[CT]
ClaI 267 ATCGAT AT^CGAT
DraIII 1635 CACTATGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 30 CGGCCG C^GGCCG
EclXI 30 CGGCCG C^GGCCG
Eco52I 30 CGGCCG C^GGCCG
Eco81I 360 CCTTAGG CC^T[ACGT]AGG
EcoRV 683 GATATC GAT^ATC
EcoT22I 1229 ATGCAT ATGCA^T
FbaI 352 TGATCA T^GATCA
HaeII 1 GGCGCT [GA]GCGC^[CT]
HgaI 40 GACGC GACGC(5/10)
HhaI 2 GCGC GCG^C
HinP1I 2 GCGC G^CGC
HincII 2538 GTTGAC GT[CT]^[GA]AC
HindII 2538 GTTGAC GT[CT]^[GA]AC
HindIII 917 AAGCTT A^AGCTT
MfeI 999 CAATTG C^AATTG
MflI 1696 GGATCC [GA]^GATC[CT]
MluNI 1043 TGGCCA TGG^CCA
MscI 1043 TGGCCA TGG^CCA
MunI 999 CAATTG C^AATTG
MvnI 42 CGCG CG^CG
NaeI 485 GCCGGC GCC^GGC
NciI 1140 CCCGG CC^[GC]GG
NdeI 780 CATATG CA^TATG
NgoMIV 485 GCCGGC G^CCGGC
NsiI 1229 ATGCAT ATGCA^T
NspV 888 TTCGAA TT^CGAA
PflMI 1915 CCAAGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 1822 GACTC GAGTC(4/5)
PpuMI 714 GGGACCT [GA]G^G[AT]CC[CT]
PshBI 242 ATTAAT AT^TAAT
Psp1406I 2157 AACGTT AA^CGTT
PstI 64 CTGCAG CTGCA^G
SapI 4 GCTCTTC GCTCTTC(1/4)
SexAI 499 ACCAGGT A^CC[AT]GGT
SfuI 888 TTCGAA TT^CGAA
SmlI 1331 CTCAAG C^T[CT][GA]AG
SphI 859 GCATGC GCATG^C
SspBI 532 TGTACA T^GTACA
SspI 2596 AATATT AAT^ATT
Van91I 1915 CCAAGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 1696 GGATCC [GA]^GATC[CT]
XmnI 2096 GAAAGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccI 1599 GTATAC GT^[AC][GT]AC
1617 GTATAC
AlwI 1697 GATCC GGATC(4/5)
1696 GGATC
BbsI 2348 GAAGAC GAAGAC(2/6)
217 GTCTTC
BfaI 462 CTAG C^TAG
1888 CTAG
BpuAI 2348 GAAGAC GAAGAC(2/6)
217 GTCTTC
BsmBI 38 GAGACG CGTCTC(1/5)
1071 GAGACG
BsmFI 714 GGGAC GGGAC(10/14)
90 GTCCC
BsrDI 1379 CATTGC GCAATG(2/0)
699 GCAATG
Bst1107I 1599 GTATAC GTA^TAC
1617 GTATAC
BstXI 865 CCAAGCAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1046 CCAGATGGTTGG
BstZ17I 1599 GTATAC GTA^TAC
1617 GTATAC
DraII 650 AGGCCCT [GA]G^G[ACGT]CC[CT]
714 GGGACCT
EaeI 30 CGGCCG [CT]^GGCC[GA]
1043 TGGCCA
EcoO109I 650 AGGCCCT [GA]G^G[ACGT]CC[CT]
714 GGGACCT
FauI 13 GCGGG CCCGC(4/6)
43 GCGGG
HapII 486 CCGG C^CGG
1141 CCGG
HpaII 486 CCGG C^CGG
1141 CCGG
MaeI 462 CTAG C^TAG
1888 CTAG
MaeII 2158 ACGT A^CGT
2307 ACGT
MspI 486 CCGG C^CGG
1141 CCGG
NcoI 254 CCATGG C^CATGG
631 CCATGG
NspI 542 GCATGT [GA]CATG^[CT]
859 GCATGC
TaqI 268 TCGA T^CGA
889 TCGA
TthHB8I 268 TCGA T^CGA
889 TCGA
XcmI 1511 CCAGGTGAACATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1908 CCATTTTCCAAGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 61 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
AspHI BbvI BmyI BpmI BsaJI
BsiHKAI BsiYI BslI BsmAI Bsp1286I
BsrI BssKI BstF5I BstNI Cac8I
Cfr13I DdeI DpnII DraI DsaI
EarI EcoRII EcoT14I Fnu4HI FokI
HaeIII HinfI HphI ItaI Ksp632I
MaeIII MboI MboII MnlI MseI
MslI MspA1I MvaI MwoI NdeII
NlaIII NlaIV PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI