##################################
# NO CUT ENZYMES ( 83 enzymes) #
##################################
Acc65I AccI AccIII AclI AflII
AgeI AhdI Alw44I AlwNI ApaLI
AscI AseI AsnI Asp718I AspEI
AspI AvrII BalI BamHI BbeI
BbrPI BfrI BglII BlnI BsaAI
BsaWI BseAI BsiWI BspDI BspEI
BssHII Bst1107I BstZ17I ClaI CpoI
DraIII Eam1105I EcoT22I FseI HindIII
HpaI KasI KpnI KspI MluI
MluNI MroI MscI NarI NdeI
NheI NotI NruI NsiI PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RsrII SacII SalI SapI SbfI
SfiI SfoI SgrAI SmaI SnaBI
Sse8387I SspI SwaI Tth111I XbaI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AatII 1258 GACGTC GACGT^C
Aor51HI 2210 AGCGCT AGC^GCT
Asp700I 2305 GAAGTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 815 TGCGCA TGC^GCA
BcgI 2492 CGAGGCTGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 69 GGATAC GTATCC(6/5)
BclI 2150 TGATCA T^GATCA
BglI 2583 GCCCACTAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI 53 GATCTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 1 CGGCCG CG[GA][CT]^CG
BsmI 1965 GAATGC GAATGC(1/-1)
Bsp1407I 1667 TGTACA T^GTACA
BspHI 2516 TCATGA T^CATGA
BsrDI 2073 CATTGC GCAATG(2/0)
BsrGI 1667 TGTACA T^GTACA
BssSI 1609 CACGAG CACGAG(-5/-1)
BstBI 944 TTCGAA TT^CGAA
BstEII 1618 GGTGACC G^GT[ACGT]ACC
BstPI 1618 GGTGACC G^GT[ACGT]ACC
DrdI 578 GACCCCCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 1 CGGCCG [CT]^GGCC[GA]
EagI 1 CGGCCG C^GGCCG
EclXI 1 CGGCCG C^GGCCG
Eco47III 2210 AGCGCT AGC^GCT
Eco52I 1 CGGCCG C^GGCCG
EcoNI 2229 CCTGAATCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1618 GGTGACC G^GT[ACGT]ACC
EcoRI 494 GAATTC G^AATTC
EcoRV 2200 GATATC GAT^ATC
FbaI 2150 TGATCA T^GATCA
FspI 815 TGCGCA TGC^GCA
MamI 53 GATCTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 937 CAATTG C^AATTG
MunI 937 CAATTG C^AATTG
NaeI 16 GCCGGC GCC^GGC
NcoI 740 CCATGG C^CATGG
NgoMIV 16 GCCGGC G^CCGGC
NspV 944 TTCGAA TT^CGAA
PflMI 291 CCAGCGATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 667 CTGCAG CTGCA^G
RcaI 2516 TCATGA T^CATGA
SacI 88 GAGCTC GAGCT^C
ScaI 1712 AGTACT AGT^ACT
SfuI 944 TTCGAA TT^CGAA
SpeI 1870 ACTAGT A^CTAGT
SphI 1040 GCATGC GCATG^C
SspBI 1667 TGTACA T^GTACA
StuI 836 AGGCCT AGG^CCT
Van91I 291 CCAGCGATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 2486 CCAATGCGAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 2305 GAAGTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AocI 1200 CCTCAGG CC^T[ACGT]AGG
1725 CCTCAGG
ApaI 1196 GGGCCC GGGCC^C
2581 GGGCCC
AspHI 88 GAGCTC G[AT]GC[AT]^C
1592 GTGCTC
AvaI 256 CTCGGG C^[CT]CG[GA]G
263 CTCGGG
BbsI 523 GAAGAC GAAGAC(2/6)
1402 GAAGAC
BbvCI 1602 CCTCAGC CCTCAGC(-5/-2)
439 GCTGAGG
BcnI 416 CCCGG CC^[GC]GG
1673 CCCGG
BlpI 1594 GCTCAGC GC^T[ACGT]AGC
2001 GCTGAGC
Bpu1102I 1594 GCTCAGC GC^T[ACGT]AGC
2001 GCTGAGC
BpuAI 523 GAAGAC GAAGAC(2/6)
1402 GAAGAC
BsaI 1052 GAGACC GGTCTC(1/5)
260 GGTCTC
BsiHKAI 88 GAGCTC G[AT]GC[AT]^C
1592 GTGCTC
BsmBI 34 GAGACG CGTCTC(1/5)
226 GAGACG
BsoBI 256 CTCGGG C^[CT]CG[GA]G
263 CTCGGG
BspLU11I 731 ACATGT A^CATGT
1757 ACATGT
BspMI 665 ACCTGC ACCTGC(4/8)
1055 ACCTGC
BsrBI 26 GAGCGG CCGCTC(-3/-3)
1513 GAGCGG
BsrFI 16 GCCGGC [GA]^CCGG[CT]
774 ACCGGC
BstXI 1608 CCACGAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2585 CCACTAGGCTGG
Bsu36I 1200 CCTCAGG CC^T[ACGT]AGG
1725 CCTCAGG
CelII 1594 GCTCAGC GC^T[ACGT]AGC
2001 GCTGAGC
Cfr10I 16 GCCGGC [GA]^CCGG[CT]
774 ACCGGC
DraI 1108 TTTAAA TTT^AAA
1572 TTTAAA
DsaI 740 CCATGG C^C[GA][CT]GG
1688 CCACGG
Eco81I 1200 CCTCAGG CC^T[ACGT]AGG
1725 CCTCAGG
HincII 768 GTTGAC GT[CT]^[GA]AC
2653 GTCAAC
HindII 768 GTTGAC GT[CT]^[GA]AC
2653 GTCAAC
MslI 606 CATCGAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
873 CACCCTTGTG
MspA1I 910 CAGCTG C[AC]G^C[GT]G
1096 CAGCTG
NciI 416 CCCGG CC^[GC]GG
1673 CCCGG
PvuII 910 CAGCTG CAG^CTG
1096 CAGCTG
SexAI 677 ACCTGGT A^CC[AT]GGT
1114 ACCTGGT
TfiI 1812 GATTC G^A[AT]TC
2232 GAATC
#########################################
# 3 OR MORE CUT ENZYMES ( 91 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI ApoI AvaII
BanI BanII BbvI BfaI BmyI
BpmI BsaHI BsaJI BseRI BsgI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrI BssKI BstAPI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII EarI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MspI MvaI MvnI MwoI NdeII
NlaIII NlaIV NspI PleI PpuMI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII