##################################
# NO CUT ENZYMES ( 117 enzymes) #
##################################
AatII Acc65I AclI AgeI Alw44I
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspI
AvaI AviII AvrII BalI BamHI
BbrPI BbvCI BciVI BcnI BglI
BlnI BlpI Bpu1102I BsaAI BsaI
BsgI BsiEI BsiWI BsoBI BspDI
BspHI BspMI BsrDI BssHII Bst1107I
BstAPI BstBI BstZ17I Bsu36I CelII
ClaI CpoI DraIII DrdI EaeI
EagI EclXI Eco47III Eco52I Eco81I
EcoNI EcoRI EcoT22I FauI FseI
FspI HindIII HpaI KpnI KspI
MluI MluNI MscI MspA1I NaeI
NciI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PleI PmaCI
PmlI PshBI Psp1406I PstI PvuI
PvuII RcaI RsrII SacII SalI
SapI SbfI ScaI SexAI SfiI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspI StuI SwaI
Tth111I Van91I XbaI XhoI XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AccI 1730 GTCTAC GT^[AC][GT]AC
AccIII 1263 TCCGGA T^CCGGA
AcsI 887 AAATTT [GA]^AATT[CT]
AflII 268 CTTAAG C^TTAAG
AflIII 791 ACATGT A^C[GA][CT]GT
AhdI 104 GACCGCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 1761 CAGACACTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 887 AAATTT [GA]^AATT[CT]
AspEI 104 GACCGCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 1076 GAGCTC G[AT]GC[AT]^C
BbeI 75 GGCGCC GGCGC^C
BbsI 1662 GAAGAC GAAGAC(2/6)
BcgI 451 GCAGGCTGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 268 CTTAAG C^TTAAG
BglII 1119 AGATCT A^GATCT
BpuAI 1662 GAAGAC GAAGAC(2/6)
BsaWI 1263 TCCGGA [AT]^CCGG[AT]
BseAI 1263 TCCGGA T^CCGGA
BsiHKAI 1076 GAGCTC G[AT]GC[AT]^C
BsmBI 642 GAGACG CGTCTC(1/5)
Bsp1407I 1111 TGTACA T^GTACA
BspEI 1263 TCCGGA T^CCGGA
BspLU11I 791 ACATGT A^CATGT
BsrBI 635 GAGCGG CCGCTC(-3/-3)
BsrFI 3 GCCGGT [GA]^CCGG[CT]
BsrGI 1111 TGTACA T^GTACA
BssSI 1720 CTCGTG CACGAG(-5/-1)
BstEII 1797 GGTAACC G^GT[ACGT]ACC
BstPI 1797 GGTAACC G^GT[ACGT]ACC
CfoI 76 GCGC GCG^C
Cfr10I 3 GCCGGT [GA]^CCGG[CT]
DraI 1885 TTTAAA TTT^AAA
Eam1105I 104 GACCGCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 893 GAAGAG CTCTTC(1/4)
EcoO65I 1797 GGTAACC G^GT[ACGT]ACC
HaeII 75 GGCGCC [GA]GCGC^[CT]
HhaI 76 GCGC GCG^C
HinP1I 76 GCGC G^CGC
KasI 75 GGCGCC G^GCGCC
Ksp632I 893 GAAGAG CTCTTC(1/4)
MaeII 645 ACGT A^CGT
MfeI 401 CAATTG C^AATTG
MroI 1263 TCCGGA T^CCGGA
MunI 401 CAATTG C^AATTG
NarI 75 GGCGCC GG^CGCC
PmeI 1884 GTTTAAAC GTTT^AAAC
SacI 1076 GAGCTC GAGCT^C
SfoI 75 GGCGCC GGC^GCC
SspBI 1111 TGTACA T^GTACA
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccII 461 CGCG CG^CG
1277 CGCG
AcyI 40 GGCGTC G[GA]^CG[CT]C
75 GGCGCC
AfaI 1112 GTAC GT^AC
1840 GTAC
BanI 75 GGCGCC G^G[CT][GA]CC
377 GGTGCC
BanII 556 GGGCTC G[GA]GC[CT]^C
1076 GAGCTC
BclI 286 TGATCA T^GATCA
1215 TGATCA
BfaI 407 CTAG C^TAG
932 CTAG
BsaBI 281 GATGTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1033 GATTGCCATC
BsaHI 40 GGCGTC G[GA]^CG[CT]C
75 GGCGCC
BsmI 607 GAATGC GAATGC(1/-1)
417 GCATTC
BsrI 920 ACTGG ACTGG(1/-1)
1399 ACTGG
BstUI 461 CGCG CG^CG
1277 CGCG
BstXI 300 CCAAGCTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
354 CCACAGGAGTGG
BstYI 1119 AGATCT [GA]^GATC[CT]
1688 AGATCC
EcoRV 1315 GATATC GAT^ATC
1585 GATATC
FbaI 286 TGATCA T^GATCA
1215 TGATCA
Hin1I 40 GGCGTC G[GA]^CG[CT]C
75 GGCGCC
MaeI 407 CTAG C^TAG
932 CTAG
MamI 281 GATGTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1033 GATTGCCATC
MflI 1119 AGATCT [GA]^GATC[CT]
1688 AGATCC
MvnI 461 CGCG CG^CG
1277 CGCG
NspI 791 ACATGT [GA]CATG^[CT]
1549 GCATGT
PpuMI 50 GGGACCC [GA]G^G[AT]CC[CT]
1015 AGGACCT
PshAI 841 GACCTCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1459 GACCGAAGTC
RsaI 1112 GTAC GT^AC
1840 GTAC
XcmI 36 CCATGGCGTCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
300 CCAAGCTGATGGTGG
XhoII 1119 AGATCT [GA]^GATC[CT]
1688 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 65 enzymes) #
#########################################
AciI AluI AlwI AvaII BbvI
BmyI BpmI BsaJI BseRI BsiYI
BslI BsmAI BsmFI Bsp1286I BssKI
BstF5I BstNI Cac8I Cfr13I DdeI
DpnII DraII DsaI EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HgaI HincII HindII HinfI HpaII
HphI ItaI MaeIII MboI MboII
MnlI MseI MslI MspI MvaI
MwoI NcoI NdeII NlaIII NlaIV
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I