##################################
# NO CUT ENZYMES ( 63 enzymes) #
##################################
AccIII AclI AflII AhdI AscI
AspEI AviII AvrII BfrI BglI
BglII BlnI BsaBI BsaI BseAI
BspEI Bst1107I BstZ17I CpoI DraI
DraIII DrdI EagI Eam1105I EclXI
Eco52I EcoRV FseI FspI HpaI
KspI MamI MfeI MluI MroI
MunI NaeI NdeI NgoMIV NheI
NotI NruI PacI PaeR7I PflMI
PmeI PpuMI PshAI Psp1406I RsrII
SacII SalI SbfI SfiI SgrAI
SnaBI SpeI Sse8387I StuI SwaI
Van91I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 56 enzymes) #
###################################
AatII 951 GACGTC GACGT^C
AccI 1485 GTCTAC GT^[AC][GT]AC
AgeI 2518 ACCGGT A^CCGGT
Alw44I 3311 GTGCAC G^TGCAC
AocI 3171 CCTGAGG CC^T[ACGT]AGG
Aor51HI 1377 AGCGCT AGC^GCT
ApaI 3534 GGGCCC GGGCC^C
ApaLI 3311 GTGCAC G^TGCAC
AseI 2856 ATTAAT AT^TAAT
AsnI 2856 ATTAAT AT^TAAT
BbeI 2046 GGCGCC GGCGC^C
BbrPI 2922 CACGTG CAC^GTG
BcgI 2035 GCAGTTCTCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 3817 GGATAC GTATCC(6/5)
BclI 587 TGATCA T^GATCA
BsiWI 3279 CGTACG C^GTACG
BsmBI 3663 GAGACG CGTCTC(1/5)
BsmI 1784 GAATGC GAATGC(1/-1)
BspHI 467 TCATGA T^CATGA
BsrBI 59 CCGCTC CCGCTC(-3/-3)
BsrFI 2518 ACCGGT [GA]^CCGG[CT]
BssHII 3287 GCGCGC G^CGCGC
BssSI 3720 CACGAG CACGAG(-5/-1)
BstAPI 512 GCAGCTCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 2509 TTCGAA TT^CGAA
BstEII 3832 GGTAACC G^GT[ACGT]ACC
BstPI 3832 GGTAACC G^GT[ACGT]ACC
Bsu36I 3171 CCTGAGG CC^T[ACGT]AGG
Cfr10I 2518 ACCGGT [GA]^CCGG[CT]
DraII 3533 AGGGCCC [GA]G^G[ACGT]CC[CT]
Eco47III 1377 AGCGCT AGC^GCT
Eco81I 3171 CCTGAGG CC^T[ACGT]AGG
EcoNI 270 CCTGTCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 3533 AGGGCCC [GA]G^G[ACGT]CC[CT]
EcoO65I 3832 GGTAACC G^GT[ACGT]ACC
EcoRI 1682 GAATTC G^AATTC
FbaI 587 TGATCA T^GATCA
HgaI 312 GACGC GACGC(5/10)
KasI 2046 GGCGCC G^GCGCC
NarI 2046 GGCGCC GG^CGCC
NspV 2509 TTCGAA TT^CGAA
PinAI 2518 ACCGGT A^CCGGT
PmaCI 2922 CACGTG CAC^GTG
PmlI 2922 CACGTG CAC^GTG
PshBI 2856 ATTAAT AT^TAAT
PvuI 711 CGATCG CGAT^CG
RcaI 467 TCATGA T^CATGA
SapI 496 GAAGAGC GCTCTTC(1/4)
ScaI 3505 AGTACT AGT^ACT
SexAI 659 ACCTGGT A^CC[AT]GGT
SfoI 2046 GGCGCC GGC^GCC
SfuI 2509 TTCGAA TT^CGAA
SmaI 767 CCCGGG CCC^GGG
SspI 575 AATATT AAT^ATT
XmaCI 767 CCCGGG C^CCGGG
XmaI 767 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 38 enzymes) #
###################################
Acc65I 1178 GGTACC G^GTACC
2521 GGTACC
AccII 1793 CGCG CG^CG
3288 CGCG
AcyI 951 GACGTC G[GA]^CG[CT]C
2046 GGCGCC
Asp700I 2502 GAAGATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2757 GAACGGATTC
Asp718I 1178 GGTACC G^GTACC
2521 GGTACC
AspI 658 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1702 GACAAGGTC
AvaI 767 CCCGGG C^[CT]CG[GA]G
2420 CCCGAG
BalI 2882 TGGCCA TGG^CCA
3490 TGGCCA
BbsI 655 GAAGAC GAAGAC(2/6)
2643 GTCTTC
BbvCI 1855 CCTCAGC CCTCAGC(-5/-2)
1884 CCTCAGC
BcnI 767 CCCGG CC^[GC]GG
768 CCGGG
BpuAI 655 GAAGAC GAAGAC(2/6)
2643 GTCTTC
BsaHI 951 GACGTC G[GA]^CG[CT]C
2046 GGCGCC
BsaWI 1733 TCCGGT [AT]^CCGG[AT]
2518 ACCGGT
BsiEI 711 CGATCG CG[GA][CT]^CG
3164 CGGTCG
BsoBI 767 CCCGGG C^[CT]CG[GA]G
2420 CCCGAG
BsrDI 3208 GCAATG GCAATG(2/0)
3244 GCAATG
BstUI 1793 CGCG CG^CG
3288 CGCG
EaeI 2882 TGGCCA [CT]^GGCC[GA]
3490 TGGCCA
EcoT22I 2936 ATGCAT ATGCA^T
3429 ATGCAT
Hin1I 951 GACGTC G[GA]^CG[CT]C
2046 GGCGCC
HincII 1274 GTTGAC GT[CT]^[GA]AC
3525 GTTGAC
HindII 1274 GTTGAC GT[CT]^[GA]AC
3525 GTTGAC
HindIII 2312 AAGCTT A^AGCTT
3930 AAGCTT
KpnI 1178 GGTACC GGTAC^C
2521 GGTACC
MluNI 2882 TGGCCA TGG^CCA
3490 TGGCCA
MscI 2882 TGGCCA TGG^CCA
3490 TGGCCA
MslI 3015 CATCCTTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
3347 CACCATGATG
MvnI 1793 CGCG CG^CG
3288 CGCG
NciI 767 CCCGG CC^[GC]GG
768 CCGGG
NcoI 451 CCATGG C^CATGG
1126 CCATGG
NsiI 2936 ATGCAT ATGCA^T
3429 ATGCAT
PflFI 658 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1702 GACAAGGTC
SacI 758 GAGCTC GAGCT^C
1810 GAGCTC
SmlI 157 CTCAAG C^T[CT][GA]AG
2826 CTTGAG
SphI 1720 GCATGC GCATG^C
2236 GCATGC
Tth111I 658 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1702 GACAAGGTC
XmnI 2502 GAAGATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2757 GAACGGATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 101 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI ApoI AspHI AvaII
BamHI BanI BanII BbvI BfaI
BlpI BmyI BpmI Bpu1102I BsaAI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I Bsp1407I
BspDI BspLU11I BspMI BsrGI BsrI
BssKI BstF5I BstNI BstXI BstYI
Cac8I CelII CfoI Cfr13I ClaI
DdeI DpnII DsaI EarI EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MspA1I MspI MvaI
MwoI NdeII NlaIII NlaIV NspI
PleI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SspBI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI
XhoII