##################################
# NO CUT ENZYMES ( 57 enzymes) #
##################################
AclI AgeI AhdI Alw44I ApaLI
AscI AseI AsnI AspEI AviII
AvrII BbrPI BlnI BsaAI BsiEI
BsiWI Bsp1407I BspDI BsrGI BssHII
Bst1107I BstZ17I ClaI CpoI EagI
Eam1105I EclXI Eco52I FseI FspI
KspI MfeI MluI MunI NdeI
NotI NruI PacI PaeR7I PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI RsrII SacII SfiI SgrAI
SmaI SnaBI SspBI SwaI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AatII 1896 GACGTC GACGT^C
Acc65I 3167 GGTACC G^GTACC
AccI 3203 GTCGAC GT^[AC][GT]AC
AccIII 2510 TCCGGA T^CCGGA
AflII 244 CTTAAG C^TTAAG
Asp718I 3167 GGTACC G^GTACC
AvaI 4142 CTCGGG C^[CT]CG[GA]G
BcgI 4350 GCATAGCAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 244 CTTAAG C^TTAAG
BglI 6987 GCCAGAGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaI 2141 GAGACC GGTCTC(1/5)
BseAI 2510 TCCGGA T^CCGGA
BsoBI 4142 CTCGGG C^[CT]CG[GA]G
BspEI 2510 TCCGGA T^CCGGA
BsrBI 2040 CCGCTC CCGCTC(-3/-3)
BstAPI 1121 GCATTGTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1031 TTCGAA TT^CGAA
DraI 7368 TTTAAA TTT^AAA
DrdI 1798 GACTTGAAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV 3634 GATATC GAT^ATC
EcoT22I 1540 ATGCAT ATGCA^T
HpaI 4621 GTTAAC GTT^AAC
KpnI 3167 GGTACC GGTAC^C
MroI 2510 TCCGGA T^CCGGA
NaeI 2354 GCCGGC GCC^GGC
NgoMIV 2354 GCCGGC G^CCGGC
NsiI 1540 ATGCAT ATGCA^T
NspV 1031 TTCGAA TT^CGAA
SalI 3203 GTCGAC G^TCGAC
SapI 3984 GCTCTTC GCTCTTC(1/4)
SbfI 2370 CCTGCAGG CCTGCA^GG
SfuI 1031 TTCGAA TT^CGAA
SpeI 4597 ACTAGT A^CTAGT
SphI 4967 GCATGC GCATG^C
Sse8387I 2370 CCTGCAGG CCTGCA^GG
SspI 3342 AATATT AAT^ATT
StuI 3964 AGGCCT AGG^CCT
XbaI 2586 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AflIII 2099 ACATGT A^C[GA][CT]GT
4676 ACATGT
Aor51HI 409 AGCGCT AGC^GCT
1929 AGCGCT
AspI 904 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
5395 GACATTGTC
BciVI 519 GGATAC GTATCC(6/5)
5325 GGATAC
BsaBI 1012 GATGAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4390 GATGGGGATC
BsmBI 2929 CGTCTC CGTCTC(1/5)
1894 GAGACG
BsmI 3113 GAATGC GAATGC(1/-1)
4275 GAATGC
BspLU11I 2099 ACATGT A^CATGT
4676 ACATGT
Eco47III 409 AGCGCT AGC^GCT
1929 AGCGCT
EcoRI 1225 GAATTC G^AATTC
3896 GAATTC
HindIII 1035 AAGCTT A^AGCTT
5644 AAGCTT
MamI 1012 GATGAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4390 GATGGGGATC
NheI 1248 GCTAGC G^CTAGC
6027 GCTAGC
PflFI 904 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
5395 GACATTGTC
PpuMI 7034 AGGACCC [GA]G^G[AT]CC[CT]
7318 GGGTCCC
ScaI 4973 AGTACT AGT^ACT
5988 AGTACT
Tth111I 904 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
5395 GACATTGTC
XcmI 3445 CCACCTTTGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
5675 CCAACCTGCAGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 145 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI AocI ApaI
ApoI Asp700I AspHI AvaII BalI
BamHI BanI BanII BbeI BbsI
BbvCI BbvI BclI BcnI BfaI
BglII BlpI BmyI BpmI Bpu1102I
BpuAI BsaHI BsaJI BsaWI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BspHI BspMI BsrDI
BsrFI BsrI BssKI BssSI BstEII
BstF5I BstNI BstPI BstUI BstXI
BstYI Bsu36I Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DraIII DsaI EaeI EarI Eco81I
EcoNI EcoO109I EcoO65I EcoRII EcoT14I
FauI FbaI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HinfI HpaII
HphI ItaI KasI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NarI NciI NcoI NdeII NlaIII
NlaIV NspI PflMI PleI PshAI
PstI PvuII RcaI RsaI SacI
Sau3AI Sau96I ScrFI SexAI SfaNI
SfcI SfoI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XhoII XmnI