##################################
# NO CUT ENZYMES ( 176 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI AscI Asp700I Asp718I
AspEI AspHI AspI AvaI AvaII
AviII AvrII BalI BanI BanII
BbeI BbrPI BbsI BbvCI BclI
BcnI BfrI BglII BlnI BlpI
BmyI Bpu1102I BpuAI BsaAI BsaBI
BsaHI BsaI BsaJI BsaWI BseAI
BseRI BsgI BsiEI BsiHKAI BsiWI
BsmBI BsmI BsoBI Bsp1286I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrFI BssHII Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstZ17I
Bsu36I Cac8I CelII CfoI Cfr10I
ClaI CpoI DraII DraIII DrdI
DsaI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRV EcoT14I FbaI FseI FspI
HaeII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HindIII HpaI
HpaII KasI KpnI KspI MaeII
MamI MfeI MluI MluNI MroI
MscI MspI MunI MvnI NaeI
NarI NciI NcoI NgoMIV NheI
NotI NruI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI Psp1406I PstI
PvuI RcaI RsrII SacI SacII
SalI SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SphI Sse8387I SspI StuI StyI
SwaI TaqI Tsp45I Tth111I TthHB8I
Van91I XbaI XhoI XmaCI XmaI
XmnI
###################################
# ONE CUT ENZYMES ( 28 enzymes) #
###################################
AseI 1036 ATTAAT AT^TAAT
AsnI 1036 ATTAAT AT^TAAT
BamHI 451 GGATCC G^GATCC
BcgI 411 GCATCTTTCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1094 GGATAC GTATCC(6/5)
BpmI 478 CTCCAG CTGGAG(16/14)
BsiYI 69 CCAACTGCGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 69 CCAACTGCGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 881 GTCTC GTCTC(1/5)
BsrDI 574 CATTGC GCAATG(2/0)
BssSI 419 CTCGTG CACGAG(-5/-1)
DdeI 579 CTAAG C^T[ACGT]AG
EaeI 88 TGGCCG [CT]^GGCC[GA]
EarI 406 GAAGAG CTCTTC(1/4)
EcoRI 1087 GAATTC G^AATTC
EcoT22I 615 ATGCAT ATGCA^T
HphI 252 TCACC GGTGA(8/7)
Ksp632I 406 GAAGAG CTCTTC(1/4)
MaeIII 1221 GTTAC ^GT[ACGT]AC
MslI 842 CACAGATATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NdeI 618 CATATG CA^TATG
NsiI 615 ATGCAT ATGCA^T
PshAI 601 GACACTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 1036 ATTAAT AT^TAAT
SapI 406 GAAGAGC GCTCTTC(1/4)
ScaI 975 AGTACT AGT^ACT
SpeI 1286 ACTAGT A^CTAGT
XcmI 327 CCAAACCCGTTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 13 enzymes) #
###################################
BglI 90 GCCGTAGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
281 GCCATAGTGGC
BsrI 604 ACTGG ACTGG(1/-1)
480 CCAGT
BssKI 636 CCAGG ^CC[ACGT]GG
706 CCTGG
BstNI 636 CCAGG CC^[AT]GG
706 CCTGG
Cfr13I 424 GGCCC G^G[ACGT]CC
998 GGGCC
DraI 1025 TTTAAA TTT^AAA
1172 TTTAAA
EcoRII 636 CCAGG ^CC[AT]GG
706 CCTGG
MvaI 636 CCAGG CC^[AT]GG
706 CCTGG
NlaIV 451 GGATCC GG[ACGT]^[ACGT]CC
639 GGCTCC
Sau96I 424 GGCCC G^G[ACGT]CC
998 GGGCC
ScrFI 636 CCAGG CC^[ACGT]GG
706 CCTGG
SfaNI 614 GATGC GCATC(5/9)
411 GCATC
TfiI 182 GATTC G^A[AT]TC
376 GATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 41 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI BbvI BfaI BsmFI Bsp1407I
BsrGI BstF5I BstYI DpnII FauI
Fnu4HI FokI HaeIII HinfI ItaI
MaeI MboI MboII MflI MnlI
MseI MspA1I MwoI NdeII NlaIII
PleI PvuII RsaI Sau3AI SfcI
SspBI Tru9I TseI Tsp509I TspRI
XhoII