##################################
# NO CUT ENZYMES ( 176 enzymes) #
##################################
AatII Acc65I AccII AccIII AciI
AclI AcsI AcyI AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI ApoI AscI Asp700I Asp718I
AspEI AspHI AspI AvaI AvaII
AviII AvrII BalI BamHI BanI
BanII BbeI BbrPI BbvCI BcgI
BciVI BclI BcnI BglI BglII
BlnI BlpI BmyI BpmI Bpu1102I
BsaAI BsaBI BsaHI BsaWI BseAI
BseRI BsiEI BsiHKAI BsiWI BsiYI
BslI BsmBI BsmFI BsmI BsoBI
Bsp1286I BspDI BspEI BspHI BsrBI
BsrFI BssHII BssSI BstAPI BstEII
BstPI BstUI BstXI Bsu36I Cac8I
CelII CfoI Cfr10I Cfr13I ClaI
CpoI DraI DraII DraIII DrdI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRI EcoRV EcoT22I FauI FbaI
FseI FspI HaeII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HindIII HpaI HpaII KasI KpnI
KspI MaeII MamI MluI MluNI
MroI MscI MspA1I MspI MvnI
NaeI NarI NciI NdeI NgoMIV
NheI NlaIV NotI NruI NsiI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI Psp1406I PstI
PvuI PvuII RcaI RsrII SacI
SacII SalI SapI Sau96I SbfI
ScaI SexAI SfiI SfoI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
StuI SwaI TfiI Tth111I Van91I
XbaI XcmI XhoI XmaCI XmaI
XmnI
###################################
# ONE CUT ENZYMES ( 29 enzymes) #
###################################
AccI 603 GTATAC GT^[AC][GT]AC
AfaI 1122 GTAC GT^AC
BsgI 935 CTGCAC GTGCAG(16/14)
Bsp1407I 1121 TGTACA T^GTACA
BspMI 817 GCAGGT ACCTGC(4/8)
BsrDI 878 CATTGC GCAATG(2/0)
BsrGI 1121 TGTACA T^GTACA
BsrI 891 CCAGT ACTGG(1/-1)
Bst1107I 603 GTATAC GTA^TAC
BstBI 395 TTCGAA TT^CGAA
BstF5I 210 GGATG GGATG(2/0)
BstYI 354 AGATCC [GA]^GATC[CT]
BstZ17I 603 GTATAC GTA^TAC
FokI 210 GGATG GGATG(9/13)
HinfI 269 GAGTC G^A[ACGT]TC
MfeI 940 CAATTG C^AATTG
MflI 354 AGATCC [GA]^GATC[CT]
MunI 940 CAATTG C^AATTG
NspV 395 TTCGAA TT^CGAA
PacI 124 TTAATTAA TTAAT^TAA
PleI 269 GAGTC GAGTC(4/5)
PshAI 199 GACTATGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsaI 1122 GTAC GT^AC
SfaNI 319 GATGC GCATC(5/9)
SfcI 996 CTACAG C^T[GA][CT]AG
SfuI 395 TTCGAA TT^CGAA
SspBI 1121 TGTACA T^GTACA
SspI 842 AATATT AAT^ATT
XhoII 354 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AflII 850 CTTAAG C^TTAAG
896 CTTAAG
AflIII 866 ACATGT A^C[GA][CT]GT
1149 ACATGT
AlwI 355 GATCC GGATC(4/5)
164 GGATC
AseI 123 ATTAAT AT^TAAT
127 ATTAAT
AsnI 123 ATTAAT AT^TAAT
127 ATTAAT
BfrI 850 CTTAAG C^TTAAG
896 CTTAAG
BsaI 464 GAGACC GGTCTC(1/5)
15 GGTCTC
BspLU11I 866 ACATGT A^CATGT
1149 ACATGT
DsaI 302 CCATGG C^C[GA][CT]GG
1091 CCATGG
EarI 1132 CTCTTC CTCTTC(1/4)
1096 GAAGAG
HaeIII 423 GGCC GG^CC
954 GGCC
HphI 180 GGTGA GGTGA(8/7)
887 TCACC
Ksp632I 1132 CTCTTC CTCTTC(1/4)
1096 GAAGAG
MslI 511 CATGACTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1144 CATATACATG
MwoI 520 GCAAAAGCAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
775 GCTAGTTGGGC
NcoI 302 CCATGG C^CATGG
1091 CCATGG
NspI 866 ACATGT [GA]CATG^[CT]
1149 ACATGT
PshBI 123 ATTAAT AT^TAAT
127 ATTAAT
Tsp45I 34 GTCAC ^GT[GC]AC
263 GTCAC
TspRI 366 CACTG CA[GC]TG[ACGT][ACGT]^
980 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 33 enzymes) #
#########################################
AluI BbsI BbvI BfaI BpuAI
BsaJI BsmAI BssKI BstNI DdeI
DpnII EcoRII EcoT14I Fnu4HI ItaI
MaeI MaeIII MboI MboII MnlI
MseI MvaI NdeII NlaIII Sau3AI
ScrFI SmlI StyI TaqI Tru9I
TseI Tsp509I TthHB8I