##################################
# NO CUT ENZYMES ( 130 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AflIII Alw44I Aor51HI
ApaI ApaLI AscI Asp718I AspI
AviII BalI BamHI BanI BbeI
BbrPI BbsI BbvCI BciVI BclI
BfrI BglII BlpI BpmI Bpu1102I
BpuAI BsaAI BsaBI BsaHI BseAI
BseRI BsgI BsiEI BsiWI BsmBI
BsmFI BspDI BspEI BspHI BspLU11I
BsrBI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstXI BstZ17I
CelII CfoI ClaI CpoI DraI
DraIII DrdI EaeI EagI EclXI
Eco47III Eco52I EcoNI EcoO65I EcoRV
EcoT22I FbaI FseI FspI HaeII
HhaI Hin1I HinP1I HincII HindII
HindIII HpaI KasI KpnI KspI
MamI MfeI MluI MluNI MroI
MscI MunI NarI NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PmaCI PmeI PmlI PpuMI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SapI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI StuI SwaI Tsp45I
Tth111I XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 61 enzymes) #
###################################
AccII 109 CGCG CG^CG
AgeI 175 ACCGGT A^CCGGT
AhdI 468 GACAAGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 260 GATCC GGATC(4/5)
AlwNI 392 CAGTTTCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 448 CCTTAGG CC^T[ACGT]AGG
AspEI 468 GACAAGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 130 GAGCAC G[AT]GC[AT]^C
AvaI 504 CTCGGG C^[CT]CG[GA]G
AvrII 40 CCTAGG C^CTAGG
BanII 146 GAGCCC G[GA]GC[CT]^C
BcgI 145 CGAGCCCGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 149 CCCGG CC^[GC]GG
BfaI 41 CTAG C^TAG
BglI 115 GCCTGTCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 40 CCTAGG C^CTAGG
BsaI 937 GAGACC GGTCTC(1/5)
BsiHKAI 130 GAGCAC G[AT]GC[AT]^C
BsmAI 937 GAGAC GTCTC(1/5)
BsoBI 504 CTCGGG C^[CT]CG[GA]G
Bsp1407I 1028 TGTACA T^GTACA
BspMI 216 GCAGGT ACCTGC(4/8)
BsrGI 1028 TGTACA T^GTACA
BstUI 109 CGCG CG^CG
BstYI 259 AGATCC [GA]^GATC[CT]
Bsu36I 448 CCTTAGG CC^T[ACGT]AGG
DraII 112 GGGGCCT [GA]G^G[ACGT]CC[CT]
Eam1105I 468 GACAAGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 423 GAAGAG CTCTTC(1/4)
Eco81I 448 CCTTAGG CC^T[ACGT]AGG
EcoO109I 112 GGGGCCT [GA]G^G[ACGT]CC[CT]
EcoRI 233 GAATTC G^AATTC
HgaI 860 GCGTC GACGC(5/10)
HphI 1127 TCACC GGTGA(8/7)
Ksp632I 423 GAAGAG CTCTTC(1/4)
MaeI 41 CTAG C^TAG
MaeII 700 ACGT A^CGT
MaeIII 353 GTTAC ^GT[ACGT]AC
MflI 259 AGATCC [GA]^GATC[CT]
MslI 1128 CACCAGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 109 CGCG CG^CG
NaeI 82 GCCGGC GCC^GGC
NciI 149 CCCGG CC^[GC]GG
NcoI 785 CCATGG C^CATGG
NdeI 369 CATATG CA^TATG
NgoMIV 82 GCCGGC G^CCGGC
NspI 1063 ACATGC [GA]CATG^[CT]
PflMI 391 CCAGTTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 175 ACCGGT A^CCGGT
PshAI 1075 GACCAATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 97 CTGCAG CTGCA^G
PvuII 360 CAGCTG CAG^CTG
SbfI 96 CCTGCAGG CCTGCA^GG
ScaI 310 AGTACT AGT^ACT
SmlI 223 CTTGAG C^T[CT][GA]AG
Sse8387I 96 CCTGCAGG CCTGCA^GG
SspBI 1028 TGTACA T^GTACA
SspI 713 AATATT AAT^ATT
Van91I 391 CCAGTTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1084 CCATTTGCTTATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 259 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AseI 1000 ATTAAT AT^TAAT
1282 ATTAAT
AsnI 1000 ATTAAT AT^TAAT
1282 ATTAAT
Asp700I 233 GAATTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1201 GAAACAATTC
BsaWI 175 ACCGGT [AT]^CCGG[AT]
208 ACCGGA
BsmI 408 GCATTC GAATGC(1/-1)
986 GCATTC
BsrDI 10 CATTGC GCAATG(2/0)
32 GCAATG
BsrFI 82 GCCGGC [GA]^CCGG[CT]
175 ACCGGT
Cfr10I 82 GCCGGC [GA]^CCGG[CT]
175 ACCGGT
DsaI 201 CCGTGG C^C[GA][CT]GG
785 CCATGG
EcoT14I 40 CCTAGG C^C[AT][AT]GG
785 CCATGG
MspA1I 15 CAGCGG C[AC]G^C[GT]G
360 CAGCTG
PleI 67 GACTC GAGTC(4/5)
226 GAGTC
PshBI 1000 ATTAAT AT^TAAT
1282 ATTAAT
SfaNI 572 GATGC GCATC(5/9)
1124 GCATC
SfcI 97 CTGCAG C^T[GA][CT]AG
357 CTACAG
StyI 40 CCTAGG C^C[AT][AT]GG
785 CCATGG
XmnI 233 GAATTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1201 GAAACAATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 50 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
AvaII BbvI BmyI BsaJI BsiYI
BslI Bsp1286I BsrI BssKI BstF5I
BstNI Cac8I Cfr13I DdeI DpnII
EcoRII FauI Fnu4HI FokI HaeIII
HapII HinfI HpaII ItaI MboI
MboII MnlI MseI MspI MvaI
MwoI NdeII NlaIII NlaIV RsaI
Sau3AI Sau96I ScrFI TaqI TfiI
Tru9I TseI Tsp509I TspRI TthHB8I