##################################
# NO CUT ENZYMES ( 125 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AflIII AgeI AhdI AlwNI
AocI Aor51HI ApaI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII AvrII BalI BbrPI BbvCI
BcgI BciVI BglI BglII BlnI
BlpI Bpu1102I BsaAI BsaWI BseAI
BseRI BsgI BsiWI BsmBI BspEI
BspHI BspLU11I BsrBI BsrDI BsrFI
BssHII Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstZ17I Bsu36I CelII
Cfr10I CpoI DraII DrdI DsaI
Eam1105I Eco47III Eco81I EcoNI EcoO109I
EcoO65I EcoRI EcoT22I FauI FseI
FspI HgaI HpaI KpnI KspI
MluI MluNI MroI MscI MvnI
NaeI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SbfI ScaI SexAI SfiI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I StuI SwaI Tth111I Van91I
XcmI XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AcyI 21 GGCGCC G[GA]^CG[CT]C
AflII 714 CTTAAG C^TTAAG
Alw44I 181 GTGCAC G^TGCAC
ApaLI 181 GTGCAC G^TGCAC
BamHI 394 GGATCC G^GATCC
BbeI 21 GGCGCC GGCGC^C
BbsI 659 GTCTTC GAAGAC(2/6)
BcnI 47 CCCGG CC^[GC]GG
BfrI 714 CTTAAG C^TTAAG
BpuAI 659 GTCTTC GAAGAC(2/6)
BsaBI 356 GATAGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 21 GGCGCC G[GA]^CG[CT]C
BsaI 1350 GAGACC GGTCTC(1/5)
BsiEI 103 CGGCCG CG[GA][CT]^CG
BsmI 144 GCATTC GAATGC(1/-1)
Bsp1407I 1523 TGTACA T^GTACA
BspDI 350 ATCGAT AT^CGAT
BspMI 1449 GCAGGT ACCTGC(4/8)
BsrGI 1523 TGTACA T^GTACA
BssSI 435 CTCGTG CACGAG(-5/-1)
ClaI 350 ATCGAT AT^CGAT
DdeI 524 CTCAG C^T[ACGT]AG
DraIII 1539 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 103 CGGCCG [CT]^GGCC[GA]
EagI 103 CGGCCG C^GGCCG
EclXI 103 CGGCCG C^GGCCG
Eco52I 103 CGGCCG C^GGCCG
EcoRV 296 GATATC GAT^ATC
HaeII 21 GGCGCC [GA]GCGC^[CT]
HapII 48 CCGG C^CGG
Hin1I 21 GGCGCC G[GA]^CG[CT]C
HincII 1028 GTTGAC GT[CT]^[GA]AC
HindII 1028 GTTGAC GT[CT]^[GA]AC
HpaII 48 CCGG C^CGG
KasI 21 GGCGCC G^GCGCC
MamI 356 GATAGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspI 48 CCGG C^CGG
NarI 21 GGCGCC GG^CGCC
NciI 47 CCCGG CC^[GC]GG
SacI 671 GAGCTC GAGCT^C
SfoI 21 GGCGCC GGC^GCC
SspBI 1523 TGTACA T^GTACA
TaqI 351 TCGA T^CGA
TthHB8I 351 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AcsI 221 GAATTT [GA]^AATT[CT]
1377 AAATTT
ApoI 221 GAATTT [GA]^AATT[CT]
1377 AAATTT
AspHI 181 GTGCAC G[AT]GC[AT]^C
671 GAGCTC
AvaI 62 CTCGGG C^[CT]CG[GA]G
162 CTCGGG
BanI 21 GGCGCC G^G[CT][GA]CC
214 GGCACC
BanII 320 GAGCCC G[GA]GC[CT]^C
671 GAGCTC
BclI 1222 TGATCA T^GATCA
1279 TGATCA
BpmI 542 CTCCAG CTGGAG(16/14)
286 CTGGAG
BsiHKAI 181 GTGCAC G[AT]GC[AT]^C
671 GAGCTC
BsoBI 62 CTCGGG C^[CT]CG[GA]G
162 CTCGGG
BstXI 375 CCATCCTGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
575 CCAGTTGATTGG
BstYI 394 GGATCC [GA]^GATC[CT]
983 GGATCT
CfoI 22 GCGC GCG^C
1196 GCGC
DraI 963 TTTAAA TTT^AAA
1356 TTTAAA
FbaI 1222 TGATCA T^GATCA
1279 TGATCA
HaeIII 88 GGCC GG^CC
104 GGCC
HhaI 22 GCGC GCG^C
1196 GCGC
HinP1I 22 GCGC G^CGC
1196 GCGC
HindIII 1035 AAGCTT A^AGCTT
1300 AAGCTT
MaeII 69 ACGT A^CGT
1091 ACGT
MfeI 310 CAATTG C^AATTG
764 CAATTG
MflI 394 GGATCC [GA]^GATC[CT]
983 GGATCT
MslI 421 CATGCCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
797 CATCCTGGTG
MunI 310 CAATTG C^AATTG
764 CAATTG
NspI 387 GCATGC [GA]CATG^[CT]
420 GCATGC
PleI 134 GACTC GAGTC(4/5)
657 GAGTC
PstI 777 CTGCAG CTGCA^G
989 CTGCAG
SapI 317 GAAGAGC GCTCTTC(1/4)
1336 GAAGAGC
SfcI 777 CTGCAG C^T[GA][CT]AG
989 CTGCAG
SmlI 714 CTTAAG C^T[CT][GA]AG
875 CTTGAG
SphI 387 GCATGC GCATG^C
420 GCATGC
SspI 1360 AATATT AAT^ATT
1461 AATATT
XhoII 394 GGATCC [GA]^GATC[CT]
983 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 56 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaII
BbvI BfaI BmyI BsaJI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrI
BssKI BstF5I BstNI Cac8I Cfr13I
DpnII EarI EcoRII EcoT14I Fnu4HI
FokI HinfI HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MnlI
MseI MspA1I MvaI MwoI NdeII
NlaIII NlaIV PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI StyI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
XbaI