##################################
# NO CUT ENZYMES ( 132 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwI Aor51HI ApaI ApaLI
AscI Asp700I Asp718I AspEI AspI
AviII AvrII BalI BamHI BanI
BbeI BbrPI BbvCI BciVI BfrI
BglI BlnI BsaAI BsaHI BsaI
BsaWI BseAI BsgI BsiWI Bsp1407I
BspEI BspHI BspLU11I BspMI BsrBI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstXI BstZ17I Cfr10I
CpoI DraI DraII DrdI Eam1105I
Eco47III EcoO109I EcoRV FauI FseI
FspI HaeII Hin1I HincII HindII
HpaI KasI KpnI MfeI MluI
MluNI MroI MscI MslI MunI
NaeI NarI NcoI NgoMIV NheI
NotI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PleI PmaCI
PmeI PmlI PpuMI PshAI Psp1406I
PstI PvuI RcaI RsrII SalI
SapI SbfI ScaI SexAI SfcI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI Tth111I
Van91I XbaI XcmI XhoI XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 56 enzymes) #
###################################
AocI 457 CCTTAGG CC^T[ACGT]AGG
AseI 1184 ATTAAT AT^TAAT
AsnI 1184 ATTAAT AT^TAAT
AvaI 110 CCCGAG C^[CT]CG[GA]G
AvaII 226 GGACC G^G[AT]CC
BanII 381 GAGCTC G[GA]GC[CT]^C
BbsI 780 GTCTTC GAAGAC(2/6)
BcgI 198 GCAAATCCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1015 TGATCA T^GATCA
BcnI 1044 CCGGG CC^[GC]GG
BfaI 308 CTAG C^TAG
BlpI 100 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 100 GCTCAGC GC^T[ACGT]AGC
BpuAI 780 GTCTTC GAAGAC(2/6)
BsaBI 482 GATAATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 153 CGGCCG CG[GA][CT]^CG
BsmBI 123 GAGACG CGTCTC(1/5)
BsmI 818 GAATGC GAATGC(1/-1)
BsoBI 110 CCCGAG C^[CT]CG[GA]G
BspDI 900 ATCGAT AT^CGAT
BsrDI 332 GCAATG GCAATG(2/0)
BstEII 428 GGTCACC G^GT[ACGT]ACC
BstPI 428 GGTCACC G^GT[ACGT]ACC
Bsu36I 457 CCTTAGG CC^T[ACGT]AGG
Cac8I 105 GCCTGC GC[ACGT]^[ACGT]GC
CelII 100 GCTCAGC GC^T[ACGT]AGC
ClaI 900 ATCGAT AT^CGAT
DraIII 1259 CACAATGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 118 CCGCGG C^C[GA][CT]GG
EaeI 153 CGGCCG [CT]^GGCC[GA]
EagI 153 CGGCCG C^GGCCG
EarI 623 CTCTTC CTCTTC(1/4)
EclXI 153 CGGCCG C^GGCCG
Eco52I 153 CGGCCG C^GGCCG
Eco81I 457 CCTTAGG CC^T[ACGT]AGG
EcoNI 40 CCTGGAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 428 GGTCACC G^GT[ACGT]ACC
EcoT14I 970 CCTTGG C^C[AT][AT]GG
EcoT22I 930 ATGCAT ATGCA^T
FbaI 1015 TGATCA T^GATCA
HgaI 125 GACGC GACGC(5/10)
HindIII 520 AAGCTT A^AGCTT
Ksp632I 623 CTCTTC CTCTTC(1/4)
KspI 118 CCGCGG CCGC^GG
MaeI 308 CTAG C^TAG
MaeII 222 ACGT A^CGT
MamI 482 GATAATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NciI 1044 CCGGG CC^[GC]GG
NdeI 405 CATATG CA^TATG
NlaIV 175 GGCTCC GG[ACGT]^[ACGT]CC
NruI 325 TCGCGA TCG^CGA
NsiI 930 ATGCAT ATGCA^T
PshBI 1184 ATTAAT AT^TAAT
SacI 381 GAGCTC GAGCT^C
SacII 118 CCGCGG CCGC^GG
StyI 970 CCTTGG C^C[AT][AT]GG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AfaI 352 GTAC GT^AC
1120 GTAC
AspHI 113 GAGCAC G[AT]GC[AT]^C
381 GAGCTC
BglII 727 AGATCT A^GATCT
1144 AGATCT
BpmI 32 CTGGAG CTGGAG(16/14)
41 CTGGAG
BsiHKAI 113 GAGCAC G[AT]GC[AT]^C
381 GAGCTC
BsiYI 40 CCTGGAGGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
204 CCCTCGACTGG
BslI 40 CCTGGAGGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
204 CCCTCGACTGG
BsmFI 546 GGGAC GGGAC(10/14)
11 GTCCC
BstF5I 651 CATCC GGATG(2/0)
277 GGATG
BstYI 727 AGATCT [GA]^GATC[CT]
1144 AGATCT
Cfr13I 226 GGACC G^G[ACGT]CC
421 GGCCC
EcoRI 924 GAATTC G^AATTC
1007 GAATTC
FokI 651 CATCC GGATG(9/13)
277 GGATG
HapII 361 CCGG C^CGG
1044 CCGG
HpaII 361 CCGG C^CGG
1044 CCGG
HphI 368 GGTGA GGTGA(8/7)
430 TCACC
MflI 727 AGATCT [GA]^GATC[CT]
1144 AGATCT
MspI 361 CCGG C^CGG
1044 CCGG
RsaI 352 GTAC GT^AC
1120 GTAC
Sau96I 226 GGACC G^G[ACGT]CC
421 GGCCC
TaqI 207 TCGA T^CGA
901 TCGA
TspRI 634 CACTG CA[GC]TG[ACGT][ACGT]^
1055 CACTG
TthHB8I 207 TCGA T^CGA
901 TCGA
XhoII 727 AGATCT [GA]^GATC[CT]
1144 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 46 enzymes) #
#########################################
AccII AciI AcsI AluI AlwNI
ApoI BbvI BmyI BsaJI BseRI
BsmAI Bsp1286I BsrI BssKI BstNI
BstUI CfoI DdeI DpnII EcoRII
Fnu4HI HaeIII HhaI HinP1I HinfI
ItaI MaeIII MboI MboII MnlI
MseI MspA1I MvaI MvnI MwoI
NdeII NlaIII PvuII Sau3AI ScrFI
SfaNI TfiI Tru9I TseI Tsp45I
Tsp509I