##################################
# NO CUT ENZYMES ( 143 enzymes) #
##################################
AatII Acc65I AccI AccIII AcyI
AflII AhdI Alw44I AlwNI AocI
Aor51HI ApaLI AscI AseI AsnI
Asp718I AspEI AspHI AvaI AviII
AvrII BalI BamHI BanI BbeI
BbrPI BbvCI BcgI BciVI BclI
BfrI BglI BlnI BlpI Bpu1102I
BsaAI BsaBI BsaHI BsaI BseAI
BsiEI BsiHKAI BsiWI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrDI BsrGI BsrI BssHII Bst1107I
BstBI BstEII BstPI BstXI BstZ17I
Bsu36I CelII CfoI ClaI CpoI
DrdI DsaI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HaeII HhaI Hin1I HinP1I
HindIII HpaI KasI KpnI KspI
MamI MfeI MluNI MroI MscI
MunI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflMI PmaCI
PmeI PmlI PshAI PshBI PvuI
RcaI RsrII SacI SacII SalI
SbfI SfaNI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI StuI SwaI
TaqI TthHB8I Van91I XbaI XcmI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AccII 44 CGCG CG^CG
AclI 102 AACGTT AA^CGTT
AcsI 851 AAATTC [GA]^AATT[CT]
AflIII 43 ACGCGT A^C[GA][CT]GT
AgeI 181 ACCGGT A^CCGGT
AlwI 569 GGATC GGATC(4/5)
ApaI 114 GGGCCC GGGCC^C
ApoI 851 AAATTC [GA]^AATT[CT]
Asp700I 847 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 501 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 244 GGACC G^G[AT]CC
BglII 1261 AGATCT A^GATCT
BpmI 617 CTCCAG CTGGAG(16/14)
BsaWI 181 ACCGGT [AT]^CCGG[AT]
BseRI 766 CTCCTC GAGGAG(10/8)
BsgI 1158 CTGCAC GTGCAG(16/14)
BsmAI 402 GTCTC GTCTC(1/5)
BsmBI 401 CGTCTC CGTCTC(1/5)
BsmI 906 GAATGC GAATGC(1/-1)
BspMI 839 ACCTGC ACCTGC(4/8)
BsrFI 181 ACCGGT [GA]^CCGG[CT]
BssSI 19 CACGAG CACGAG(-5/-1)
BstAPI 193 GCACCCGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 44 CGCG CG^CG
BstYI 1261 AGATCT [GA]^GATC[CT]
Cfr10I 181 ACCGGT [GA]^CCGG[CT]
DraI 1392 TTTAAA TTT^AAA
DraIII 194 CACCCGGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 924 CTCTTC CTCTTC(1/4)
EcoT14I 610 CCTTGG C^C[AT][AT]GG
HincII 324 GTCAAC GT[CT]^[GA]AC
HindII 324 GTCAAC GT[CT]^[GA]AC
Ksp632I 924 CTCTTC CTCTTC(1/4)
MflI 1261 AGATCT [GA]^GATC[CT]
MluI 43 ACGCGT A^CGCGT
MslI 1360 CATCTTTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 44 CGCG CG^CG
NspI 1334 GCATGT [GA]CATG^[CT]
PflFI 501 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 181 ACCGGT A^CCGGT
PpuMI 243 GGGACCT [GA]G^G[AT]CC[CT]
Psp1406I 102 AACGTT AA^CGTT
PstI 1278 CTGCAG CTGCA^G
SapI 923 GCTCTTC GCTCTTC(1/4)
ScaI 1206 AGTACT AGT^ACT
SexAI 246 ACCTGGT A^CC[AT]GGT
StyI 610 CCTTGG C^C[AT][AT]GG
TspRI 176 CAGTG CA[GC]TG[ACGT][ACGT]^
Tth111I 501 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoII 1261 AGATCT [GA]^GATC[CT]
XmnI 847 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
BanII 114 GGGCCC G[GA]GC[CT]^C
921 GGGCTC
BfaI 1067 CTAG C^TAG
1342 CTAG
BsmFI 228 GGGAC GGGAC(10/14)
243 GGGAC
BstF5I 236 CATCC GGATG(2/0)
303 CATCC
DraII 113 GGGGCCC [GA]G^G[ACGT]CC[CT]
243 GGGACCT
EaeI 119 CGGCCA [CT]^GGCC[GA]
796 TGGCCG
EcoO109I 113 GGGGCCC [GA]G^G[ACGT]CC[CT]
243 GGGACCT
FokI 236 CATCC GGATG(9/13)
303 CATCC
HgaI 42 GACGC GACGC(5/10)
45 GCGTC
HphI 1239 GGTGA GGTGA(8/7)
538 TCACC
MaeI 1067 CTAG C^TAG
1342 CTAG
MspA1I 319 CAGCTG C[AC]G^C[GT]G
620 CAGCTG
PleI 299 GACTC GAGTC(4/5)
615 GACTC
PvuII 319 CAGCTG CAG^CTG
620 CAGCTG
SfcI 188 CTACAG C^T[GA][CT]AG
1278 CTGCAG
SspI 1230 AATATT AAT^ATT
1294 AATATT
TfiI 473 GATTC G^A[AT]TC
553 GAATC
#########################################
# 3 OR MORE CUT ENZYMES ( 47 enzymes) #
#########################################
AciI AfaI AluI BbsI BbvI
BcnI BmyI BpuAI BsaJI BsiYI
BslI Bsp1286I BssKI BstNI Cac8I
Cfr13I DdeI DpnII EcoRII FauI
Fnu4HI HaeIII HapII HinfI HpaII
ItaI MaeII MaeIII MboI MboII
MnlI MseI MspI MvaI MwoI
NciI NdeII NlaIII NlaIV RsaI
Sau3AI Sau96I ScrFI Tru9I TseI
Tsp45I Tsp509I