##################################
# NO CUT ENZYMES ( 104 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII Alw44I AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp718I
AvrII BalI BbeI BbvCI BcgI
BciVI BclI BfrI BglII BlnI
BlpI Bpu1102I BsaBI BsaHI BsaI
BseAI BsiEI BsiWI BsmBI BspDI
BspEI BspHI BspLU11I BssSI Bst1107I
BstEII BstPI BstZ17I Bsu36I CelII
ClaI CpoI DraII DraIII EagI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRV EcoT22I FbaI
FseI HaeII Hin1I HindIII HpaI
KasI KpnI MamI MluI MluNI
MroI MscI NarI NdeI NotI
NruI NsiI PacI PaeR7I PmeI
PpuMI PshAI PshBI Psp1406I PstI
PvuI RcaI RsrII SalI SbfI
ScaI SfiI SfoI SgrAI SmaI
SnaBI SpeI Sse8387I SspI StuI
SwaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AccI 141 GTCTAC GT^[AC][GT]AC
AgeI 1556 ACCGGT A^CCGGT
AhdI 36 GACACCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I 473 GAAAACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 36 GACACCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1828 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 214 TGCGCA TGC^GCA
BbrPI 1821 CACGTG CAC^GTG
BbsI 318 GTCTTC GAAGAC(2/6)
BpuAI 318 GTCTTC GAAGAC(2/6)
BsaAI 1821 CACGTG [CT]AC^GT[GA]
BsaWI 1556 ACCGGT [AT]^CCGG[AT]
Bsp1407I 1817 TGTACA T^GTACA
BsrDI 233 CATTGC GCAATG(2/0)
BsrGI 1817 TGTACA T^GTACA
BssHII 466 GCGCGC G^CGCGC
BstBI 321 TTCGAA TT^CGAA
DraI 1017 TTTAAA TTT^AAA
DrdI 1329 GACCATGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 36 GACACCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FspI 214 TGCGCA TGC^GCA
HgaI 976 GACGC GACGC(5/10)
HincII 303 GTCAAC GT[CT]^[GA]AC
HindII 303 GTCAAC GT[CT]^[GA]AC
KspI 65 CCGCGG CCGC^GG
MfeI 805 CAATTG C^AATTG
MunI 805 CAATTG C^AATTG
NaeI 14 GCCGGC GCC^GGC
NcoI 313 CCATGG C^CATGG
NgoMIV 14 GCCGGC G^CCGGC
NspI 709 GCATGC [GA]CATG^[CT]
NspV 321 TTCGAA TT^CGAA
PflFI 1828 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 1556 ACCGGT A^CCGGT
PmaCI 1821 CACGTG CAC^GTG
PmlI 1821 CACGTG CAC^GTG
PvuII 772 CAGCTG CAG^CTG
SacII 65 CCGCGG CCGC^GG
SexAI 39 ACCAGGT A^CC[AT]GGT
SfuI 321 TTCGAA TT^CGAA
SmlI 1281 CTCAAG C^T[CT][GA]AG
SphI 709 GCATGC GCATG^C
SspBI 1817 TGTACA T^GTACA
Tth111I 1828 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 431 TCTAGA T^CTAGA
XcmI 268 CCAACATTTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 473 GAAAACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AcsI 324 GAATTC [GA]^AATT[CT]
452 GAATTC
AfaI 1115 GTAC GT^AC
1818 GTAC
AflIII 1820 ACACGT A^C[GA][CT]GT
1822 ACGTGT
ApoI 324 GAATTC [GA]^AATT[CT]
452 GAATTC
AvaI 136 CCCGAG C^[CT]CG[GA]G
1518 CCCGAG
BamHI 1054 GGATCC G^GATCC
1765 GGATCC
BanI 1259 GGTGCC G^G[CT][GA]CC
1447 GGTGCC
BglI 9 GCCGGGCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1363 GCCGCAGTGGC
BseRI 1431 CTCCTC GAGGAG(10/8)
1480 CTCCTC
BsgI 821 CTGCAC GTGCAG(16/14)
1083 GTGCAG
BsmI 717 GAATGC GAATGC(1/-1)
1130 GAATGC
BsoBI 136 CCCGAG C^[CT]CG[GA]G
1518 CCCGAG
BsrBI 1451 CCGCTC CCGCTC(-3/-3)
791 GAGCGG
BsrFI 14 GCCGGC [GA]^CCGG[CT]
1556 ACCGGT
BstAPI 168 GCAGTGGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
206 GCAGCAGGTGC
BstXI 545 CCAGGTAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1689 CCACCCCTGTGG
BstYI 1054 GGATCC [GA]^GATC[CT]
1765 GGATCC
Cfr10I 14 GCCGGC [GA]^CCGG[CT]
1556 ACCGGT
DsaI 65 CCGCGG C^C[GA][CT]GG
313 CCATGG
EaeI 7 TGGCCG [CT]^GGCC[GA]
1361 TGGCCG
EcoRI 324 GAATTC G^AATTC
452 GAATTC
EcoT14I 313 CCATGG C^C[AT][AT]GG
925 CCAAGG
MflI 1054 GGATCC [GA]^GATC[CT]
1765 GGATCC
MseI 259 TTAA T^TAA
1018 TTAA
MspA1I 65 CCGCGG C[AC]G^C[GT]G
772 CAGCTG
NheI 1390 GCTAGC G^CTAGC
1408 GCTAGC
PflMI 268 CCAACATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1135 CCATCGTGTGG
RsaI 1115 GTAC GT^AC
1818 GTAC
SacI 600 GAGCTC GAGCT^C
1742 GAGCTC
SfcI 1322 CTACAG C^T[GA][CT]AG
1858 CTGTAG
StyI 313 CCATGG C^C[AT][AT]GG
925 CCAAGG
TfiI 428 GATTC G^A[AT]TC
1173 GAATC
Tru9I 259 TTAA T^TAA
1018 TTAA
Van91I 268 CCAACATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1135 CCATCGTGTGG
XhoII 1054 GGATCC [GA]^GATC[CT]
1765 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 72 enzymes) #
#########################################
AccII AciI AluI AlwI AlwNI
AspHI AvaII BanII BbvI BcnI
BfaI BmyI BpmI BsaJI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspMI BsrI BssKI BstF5I BstNI
BstUI Cac8I CfoI Cfr13I DdeI
DpnII EarI EcoRII FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MnlI MslI MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PleI SapI Sau3AI Sau96I ScrFI
SfaNI TaqI TseI Tsp45I Tsp509I
TspRI TthHB8I