##################################
# NO CUT ENZYMES ( 149 enzymes) #
##################################
AatII AccI AccIII AclI AflIII
AgeI AhdI Alw44I AlwI AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI AspEI AviII AvrII BalI
BamHI BanII BbrPI BcgI BciVI
BclI BglII BlnI BlpI Bpu1102I
BsaAI BsaBI BsaI BsaWI BseAI
BsgI BsiEI BsiWI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrFI BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstYI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DpnII DraII DraIII DrdI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRI EcoRV EcoT22I FauI FbaI
FseI FspI HgaI HincII HindII
HindIII HpaI KspI MaeII MamI
MboI MfeI MflI MluI MluNI
MroI MscI MspA1I MunI NaeI
NcoI NdeI NdeII NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PinAI PleI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI PvuII RcaI RsrII
SacI SacII SalI SapI Sau3AI
SbfI ScaI SexAI SfiI SfuI
SgrAI SmaI SnaBI SphI Sse8387I
SspBI SspI SwaI XbaI XcmI
XhoI XhoII XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
Acc65I 168 GGTACC G^GTACC
AcsI 1165 GAATTT [GA]^AATT[CT]
AcyI 9 GGCGCC G[GA]^CG[CT]C
AflII 283 CTTAAG C^TTAAG
ApoI 1165 GAATTT [GA]^AATT[CT]
Asp700I 299 GAAAACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 168 GGTACC G^GTACC
AspHI 546 GAGCAC G[AT]GC[AT]^C
AspI 732 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 341 CTCGGG C^[CT]CG[GA]G
BbeI 9 GGCGCC GGCGC^C
BbsI 775 GAAGAC GAAGAC(2/6)
BcnI 711 CCGGG CC^[GC]GG
BfrI 283 CTTAAG C^TTAAG
BglI 197 GCCAGGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 775 GAAGAC GAAGAC(2/6)
BsaHI 9 GGCGCC G[GA]^CG[CT]C
BsiHKAI 546 GAGCAC G[AT]GC[AT]^C
BsmBI 568 CGTCTC CGTCTC(1/5)
BsoBI 341 CTCGGG C^[CT]CG[GA]G
BstAPI 365 GCAGGAAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 581 CCATATTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraI 808 TTTAAA TTT^AAA
DsaI 764 CCACGG C^C[GA][CT]GG
EarI 571 CTCTTC CTCTTC(1/4)
Hin1I 9 GGCGCC G[GA]^CG[CT]C
KasI 9 GGCGCC G^GCGCC
KpnI 168 GGTACC GGTAC^C
Ksp632I 571 CTCTTC CTCTTC(1/4)
NarI 9 GGCGCC GG^CGCC
NciI 711 CCGGG CC^[GC]GG
NspI 991 GCATGT [GA]CATG^[CT]
PflFI 732 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 764 CCACGGCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SfcI 525 CTACAG C^T[GA][CT]AG
SfoI 9 GGCGCC GGC^GCC
SpeI 438 ACTAGT A^CTAGT
StuI 143 AGGCCT AGG^CCT
Tth111I 732 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 764 CCACGGCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 299 GAAAACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccII 446 CGCG CG^CG
820 CGCG
AfaI 169 GTAC GT^AC
442 GTAC
AlwNI 41 CAGTCCCTG CAG[ACGT][ACGT][ACGT]^CTG
538 CAGAGCCTG
AvaII 534 GGACC G^G[AT]CC
1110 GGTCC
BbvCI 32 CCTCAGC CCTCAGC(-5/-2)
405 CCTCAGC
BfaI 439 CTAG C^TAG
910 CTAG
BpmI 394 CTGGAG CTGGAG(16/14)
415 CTGGAG
BseRI 617 CTCCTC GAGGAG(10/8)
319 GAGGAG
BsrDI 1018 CATTGC GCAATG(2/0)
470 GCAATG
BstF5I 756 CATCC GGATG(2/0)
1189 GGATG
BstUI 446 CGCG CG^CG
820 CGCG
EcoT14I 462 CCTTGG C^C[AT][AT]GG
485 CCAAGG
FokI 756 CATCC GGATG(9/13)
1189 GGATG
HaeII 9 GGCGCC [GA]GCGC^[CT]
882 GGCGCT
HapII 13 CCGG C^CGG
711 CCGG
HpaII 13 CCGG C^CGG
711 CCGG
MaeI 439 CTAG C^TAG
910 CTAG
MslI 351 CACTGAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
582 CATATTCATG
MspI 13 CCGG C^CGG
711 CCGG
MvnI 446 CGCG CG^CG
820 CGCG
RsaI 169 GTAC GT^AC
442 GTAC
SfaNI 324 GATGC GCATC(5/9)
595 GATGC
SmlI 103 CTTGAG C^T[CT][GA]AG
283 CTTAAG
StyI 462 CCTTGG C^C[AT][AT]GG
485 CCAAGG
Tsp509I 864 AATT ^AATT
1166 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 43 enzymes) #
#########################################
AciI AluI BanI BbvI BmyI
BsaJI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrI BssKI BstNI Cac8I
CfoI Cfr13I DdeI EcoRII Fnu4HI
HaeIII HhaI HinP1I HinfI HphI
ItaI MaeIII MboII MnlI MseI
MvaI MwoI NlaIII NlaIV Sau96I
ScrFI TaqI TfiI Tru9I TseI
Tsp45I TspRI TthHB8I