##################################
# NO CUT ENZYMES ( 99 enzymes) #
##################################
AatII AccIII AflII AgeI AhdI
Alw44I AlwNI Aor51HI ApaI ApaLI
AscI AseI AsnI AspEI AspI
AviII AvrII BamHI BbvCI BclI
BcnI BfaI BfrI BglII BlnI
BlpI Bpu1102I BsaBI BseAI BsiWI
BsmI BspDI BspEI BsrDI BsrFI
BstAPI BstBI BstEII BstPI CelII
Cfr10I ClaI CpoI DrdI Eam1105I
Eco47III EcoO65I FbaI FseI FspI
HgaI HincII HindII HpaI MaeI
MamI MfeI MluI MroI MunI
NaeI NciI NdeI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PflFI PinAI PmeI PshAI PshBI
PstI PvuI PvuII RsrII SacI
SalI SbfI SexAI SfiI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspI StuI SwaI Tth111I
XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 71 enzymes) #
###################################
Acc65I 831 GGTACC G^GTACC
AccI 824 GTATAC GT^[AC][GT]AC
AclI 1430 AACGTT AA^CGTT
AcyI 1190 GGCGCC G[GA]^CG[CT]C
Asp700I 1174 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 831 GGTACC G^GTACC
AspHI 191 GTGCTC G[AT]GC[AT]^C
AvaI 241 CTCGGG C^[CT]CG[GA]G
AvaII 355 GGACC G^G[AT]CC
BalI 1341 TGGCCA TGG^CCA
BanII 244 GGGCTC G[GA]GC[CT]^C
BbeI 1190 GGCGCC GGCGC^C
BbrPI 747 CACGTG CAC^GTG
BbsI 275 GAAGAC GAAGAC(2/6)
BciVI 1124 GTATCC GTATCC(6/5)
BglI 227 GCCACGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 275 GAAGAC GAAGAC(2/6)
BsaAI 747 CACGTG [CT]AC^GT[GA]
BsaHI 1190 GGCGCC G[GA]^CG[CT]C
BsaI 299 GAGACC GGTCTC(1/5)
BsaWI 1182 TCCGGT [AT]^CCGG[AT]
BseRI 308 GAGGAG GAGGAG(10/8)
BsgI 572 GTGCAG GTGCAG(16/14)
BsiHKAI 191 GTGCTC G[AT]GC[AT]^C
BsmBI 1572 CGTCTC CGTCTC(1/5)
BsoBI 241 CTCGGG C^[CT]CG[GA]G
Bsp1407I 1293 TGTACA T^GTACA
BspLU11I 1624 ACATGT A^CATGT
BsrBI 1157 GAGCGG CCGCTC(-3/-3)
BsrGI 1293 TGTACA T^GTACA
BssSI 881 CACGAG CACGAG(-5/-1)
Bst1107I 824 GTATAC GTA^TAC
BstZ17I 824 GTATAC GTA^TAC
DraI 1666 TTTAAA TTT^AAA
DraII 354 AGGACCT [GA]G^G[ACGT]CC[CT]
DraIII 1012 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 1327 CGGCCG C^GGCCG
EclXI 1327 CGGCCG C^GGCCG
Eco52I 1327 CGGCCG C^GGCCG
EcoNI 346 CCTGCGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 354 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoRI 293 GAATTC G^AATTC
EcoRV 1385 GATATC GAT^ATC
EcoT14I 339 CCATGG C^C[AT][AT]GG
EcoT22I 1133 ATGCAT ATGCA^T
HaeII 1190 GGCGCC [GA]GCGC^[CT]
Hin1I 1190 GGCGCC G[GA]^CG[CT]C
HindIII 537 AAGCTT A^AGCTT
KasI 1190 GGCGCC G^GCGCC
KpnI 831 GGTACC GGTAC^C
KspI 199 CCGCGG CCGC^GG
MluNI 1341 TGGCCA TGG^CCA
MscI 1341 TGGCCA TGG^CCA
NarI 1190 GGCGCC GG^CGCC
NcoI 339 CCATGG C^CATGG
NsiI 1133 ATGCAT ATGCA^T
PflMI 505 CCAGTGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 747 CACGTG CAC^GTG
PmlI 747 CACGTG CAC^GTG
PpuMI 354 AGGACCT [GA]G^G[AT]CC[CT]
Psp1406I 1430 AACGTT AA^CGTT
SacII 199 CCGCGG CCGC^GG
SapI 193 GCTCTTC GCTCTTC(1/4)
ScaI 1395 AGTACT AGT^ACT
SfcI 1212 CTACAG C^T[GA][CT]AG
SfoI 1190 GGCGCC GGC^GCC
SmlI 534 CTCAAG C^T[CT][GA]AG
SspBI 1293 TGTACA T^GTACA
StyI 339 CCATGG C^C[AT][AT]GG
Van91I 505 CCAGTGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 1174 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AflIII 746 ACACGT A^C[GA][CT]GT
1624 ACATGT
AlwI 577 GATCC GGATC(4/5)
1516 GATCC
AocI 739 CCTAAGG CC^T[ACGT]AGG
1244 CCTCAGG
BcgI 126 GCAGCGAGGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
164 GCAGCGAGGTCG
BsiEI 1184 CGGTCG CG[GA][CT]^CG
1327 CGGCCG
BsmFI 1241 GTCCC GGGAC(10/14)
1252 GTCCC
BspHI 909 TCATGA T^CATGA
1170 TCATGA
BssHII 182 GCGCGC G^CGCGC
184 GCGCGC
BssKI 704 CCAGG ^CC[ACGT]GG
1227 CCAGG
BstNI 704 CCAGG CC^[AT]GG
1227 CCAGG
BstXI 846 CCACACAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1227 CCAGGGGGTTGG
BstYI 576 AGATCC [GA]^GATC[CT]
1515 AGATCC
Bsu36I 739 CCTAAGG CC^T[ACGT]AGG
1244 CCTCAGG
Eco81I 739 CCTAAGG CC^T[ACGT]AGG
1244 CCTCAGG
EcoRII 704 CCAGG ^CC[AT]GG
1227 CCAGG
MflI 576 AGATCC [GA]^GATC[CT]
1515 AGATCC
MslI 847 CACACAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
928 CATCTTGGTG
MvaI 704 CCAGG CC^[AT]GG
1227 CCAGG
RcaI 909 TCATGA T^CATGA
1170 TCATGA
ScrFI 704 CCAGG CC^[ACGT]GG
1227 CCAGG
XhoII 576 AGATCC [GA]^GATC[CT]
1515 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 67 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
ApoI BanI BbvI BmyI BpmI
BsaJI BsiYI BslI BsmAI Bsp1286I
BspMI BsrI BstF5I BstUI Cac8I
CfoI Cfr13I DdeI DpnII DsaI
EaeI EarI FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeII
MaeIII MboI MboII MnlI MseI
MspA1I MspI MvnI MwoI NdeII
NlaIII NlaIV NspI PleI RsaI
Sau3AI Sau96I SfaNI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XcmI