##################################
# NO CUT ENZYMES ( 115 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AfaI AflII AflIII AgeI AhdI
AlwI AocI Aor51HI AscI Asp718I
AspEI AspI AviII AvrII BamHI
BbeI BciVI BclI BfrI BglII
BlnI BlpI Bpu1102I BsaBI BseAI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspEI BspLU11I BsrGI BssSI Bst1107I
BstBI BstEII BstPI BstYI BstZ17I
Bsu36I CelII ClaI DpnII DraIII
DrdI Eam1105I Eco47III Eco81I EcoO65I
EcoRV EcoT22I FbaI FseI FspI
HincII HindII HindIII HpaI KasI
KpnI MamI MboI MfeI MflI
MluI MroI MunI NarI NdeI
NdeII NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PinAI PmeI PshAI Psp1406I PvuI
RsaI SacI SalI SapI Sau3AI
SbfI ScaI SfiI SfoI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TaqI
Tth111I TthHB8I XbaI XhoI XhoII
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AcsI 1130 GAATTC [GA]^AATT[CT]
AcyI 224 GGCGTC G[GA]^CG[CT]C
Alw44I 180 GTGCAC G^TGCAC
AlwNI 288 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 1079 GGGCCC GGGCC^C
ApaLI 180 GTGCAC G^TGCAC
ApoI 1130 GAATTC [GA]^AATT[CT]
AseI 1372 ATTAAT AT^TAAT
AsnI 1372 ATTAAT AT^TAAT
Asp700I 1460 GAATGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 180 GTGCAC G[AT]GC[AT]^C
BbrPI 16 CACGTG CAC^GTG
BbsI 485 GAAGAC GAAGAC(2/6)
BbvCI 942 CCTCAGC CCTCAGC(-5/-2)
BcgI 244 CGACCGGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BpmI 192 CTGGAG CTGGAG(16/14)
BpuAI 485 GAAGAC GAAGAC(2/6)
BsaAI 16 CACGTG [CT]AC^GT[GA]
BsaHI 224 GGCGTC G[GA]^CG[CT]C
BsaI 782 GGTCTC GGTCTC(1/5)
BsiHKAI 180 GTGCAC G[AT]GC[AT]^C
BspHI 925 TCATGA T^CATGA
BspMI 217 ACCTGC ACCTGC(4/8)
BsrBI 1597 GAGCGG CCGCTC(-3/-3)
BsrDI 589 GCAATG GCAATG(2/0)
BstAPI 692 GCACAAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 766 CCATGCAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CpoI 229 CGGACCG CG^G[AT]CCG
EagI 715 CGGCCG C^GGCCG
EclXI 715 CGGCCG C^GGCCG
Eco52I 715 CGGCCG C^GGCCG
EcoNI 818 CCTCCCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 1130 GAATTC G^AATTC
HgaI 225 GCGTC GACGC(5/10)
Hin1I 224 GGCGTC G[GA]^CG[CT]C
HphI 509 GGTGA GGTGA(8/7)
KspI 426 CCGCGG CCGC^GG
NcoI 103 CCATGG C^CATGG
PleI 1280 GACTC GAGTC(4/5)
PmaCI 16 CACGTG CAC^GTG
PmlI 16 CACGTG CAC^GTG
PpuMI 1288 AGGACCT [GA]G^G[AT]CC[CT]
PshBI 1372 ATTAAT AT^TAAT
RcaI 925 TCATGA T^CATGA
RsrII 229 CGGACCG CG^G[AT]CCG
SacII 426 CCGCGG CCGC^GG
SexAI 493 ACCTGGT A^CC[AT]GGT
SmlI 462 CTCAAG C^T[CT][GA]AG
TfiI 626 GATTC G^A[AT]TC
XmnI 1460 GAATGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AvaI 550 CCCGGG C^[CT]CG[GA]G
838 CCCGGG
BanI 581 GGTGCC G^G[CT][GA]CC
833 GGTGCC
BglI 460 GCCTCAAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1024 GCCAGAAGGGC
BsaWI 232 ACCGGA [AT]^CCGG[AT]
732 ACCGGA
BsgI 13 CTGCAC GTGCAG(16/14)
1364 CTGCAC
BsoBI 550 CCCGGG C^[CT]CG[GA]G
838 CCCGGG
DraI 1314 TTTAAA TTT^AAA
1661 TTTAAA
EarI 357 GAAGAG CTCTTC(1/4)
1100 GAAGAG
HinfI 626 GATTC G^A[ACGT]TC
1280 GACTC
Ksp632I 357 GAAGAG CTCTTC(1/4)
1100 GAAGAG
MaeII 17 ACGT A^CGT
1553 ACGT
MslI 173 CATCACGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
767 CATGCAAGTG
NaeI 717 GCCGGC GCC^GGC
1048 GCCGGC
NgoMIV 717 GCCGGC G^CCGGC
1048 GCCGGC
PflMI 113 CCAGAGCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
562 CCAAAGAATGG
PstI 70 CTGCAG CTGCA^G
198 CTGCAG
SmaI 550 CCCGGG CCC^GGG
838 CCCGGG
Tsp45I 954 GTGAC ^GT[GC]AC
1653 GTGAC
TspRI 47 CACTG CA[GC]TG[ACGT][ACGT]^
519 CAGTG
Van91I 113 CCAGAGCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
562 CCAAAGAATGG
XmaCI 550 CCCGGG C^CCGGG
838 CCCGGG
XmaI 550 CCCGGG C^CCGGG
838 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 71 enzymes) #
#########################################
AccII AciI AluI AvaII BalI
BanII BbvI BcnI BfaI BmyI
BsaJI BseRI BsiEI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrFI BsrI
BssHII BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr10I Cfr13I DdeI
DraII DsaI EaeI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HpaII
ItaI MaeI MaeIII MboII MluNI
MnlI MscI MseI MspA1I MspI
MvaI MvnI MwoI NciI NlaIII
NlaIV PvuII Sau96I ScrFI SfaNI
SfcI StyI Tru9I TseI Tsp509I
XcmI