##################################
# NO CUT ENZYMES ( 167 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AgeI AhdI Alw44I AlwI
AlwNI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvaI AviII AvrII
BalI BamHI BanI BbeI BbsI
BbvCI BcgI BciVI BclI BcnI
BglI BglII BlnI BlpI Bpu1102I
BpuAI BsaBI BsaHI BsaWI BseAI
BseRI BsiEI BsiWI BsoBI Bsp1407I
BspDI BspEI BspLU11I BsrBI BsrDI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstF5I BstXI BstYI BstZ17I
Bsu36I CelII ClaI CpoI DpnII
DraI DraII DraIII DrdI DsaI
EaeI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoRI EcoRV EcoT14I EcoT22I FbaI
FokI FseI FspI Hin1I HindIII
HpaI KasI KpnI Ksp632I KspI
MamI MboI MboII MflI MluNI
MroI MscI MslI NaeI NarI
NciI NcoI NdeI NdeII NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PleI PmeI PpuMI PshAI
PshBI Psp1406I PvuI RsrII SacII
SalI SapI Sau3AI SbfI ScaI
SexAI SfaNI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI StyI
SwaI TaqI Tth111I TthHB8I Van91I
XbaI XcmI XhoI XhoII XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AcsI 610 AAATTC [GA]^AATT[CT]
AfaI 270 GTAC GT^AC
AflII 210 CTTAAG C^TTAAG
ApoI 610 AAATTC [GA]^AATT[CT]
AspHI 87 GAGCTC G[AT]GC[AT]^C
AvaII 438 GGACC G^G[AT]CC
BanII 87 GAGCTC G[GA]GC[CT]^C
BbrPI 288 CACGTG CAC^GTG
BfrI 210 CTTAAG C^TTAAG
BmyI 87 GAGCTC G[AGT]GC[ACT]^C
BpmI 90 CTCCAG CTGGAG(16/14)
BsaAI 288 CACGTG [CT]AC^GT[GA]
BsaI 692 GGTCTC GGTCTC(1/5)
BsiHKAI 87 GAGCTC G[AT]GC[AT]^C
BsmBI 101 CGTCTC CGTCTC(1/5)
BsmI 498 GCATTC GAATGC(1/-1)
Bsp1286I 87 GAGCTC G[AGT]GC[ACT]^C
BsrFI 643 GCCGGT [GA]^CCGG[CT]
BsrI 748 ACTGG ACTGG(1/-1)
BstEII 532 GGTCACC G^GT[ACGT]ACC
BstPI 532 GGTCACC G^GT[ACGT]ACC
Cfr10I 643 GCCGGT [GA]^CCGG[CT]
EcoO65I 532 GGTCACC G^GT[ACGT]ACC
HaeII 519 GGCGCT [GA]GCGC^[CT]
HapII 644 CCGG C^CGG
HgaI 16 GACGC GACGC(5/10)
HincII 148 GTTGAC GT[CT]^[GA]AC
HindII 148 GTTGAC GT[CT]^[GA]AC
HinfI 412 GATTC G^A[ACGT]TC
HpaII 644 CCGG C^CGG
MfeI 203 CAATTG C^AATTG
MluI 50 ACGCGT A^CGCGT
MspI 644 CCGG C^CGG
MunI 203 CAATTG C^AATTG
NlaIV 64 GGAACC GG[ACGT]^[ACGT]CC
PmaCI 288 CACGTG CAC^GTG
PmlI 288 CACGTG CAC^GTG
PstI 490 CTGCAG CTGCA^G
RsaI 270 GTAC GT^AC
SacI 87 GAGCTC GAGCT^C
TfiI 412 GATTC G^A[AT]TC
###################################
# TWO CUT ENZYMES ( 12 enzymes) #
###################################
AflIII 50 ACGCGT A^C[GA][CT]GT
289 ACGTGT
BfaI 314 CTAG C^TAG
516 CTAG
BsgI 121 GTGCAG GTGCAG(16/14)
297 GTGCAG
BsmAI 102 GTCTC GTCTC(1/5)
693 GTCTC
BsmFI 58 GGGAC GGGAC(10/14)
422 GGGAC
BspHI 580 TCATGA T^CATGA
648 TCATGA
Cfr13I 45 GGGCC G^G[ACGT]CC
438 GGACC
HphI 368 TCACC GGTGA(8/7)
534 TCACC
MaeI 314 CTAG C^TAG
516 CTAG
RcaI 580 TCATGA T^CATGA
648 TCATGA
Sau96I 45 GGGCC G^G[ACGT]CC
438 GGACC
SmlI 84 CTTGAG C^T[CT][GA]AG
210 CTTAAG
#########################################
# 3 OR MORE CUT ENZYMES ( 38 enzymes) #
#########################################
AccII AciI AluI BbvI BsaJI
BsiYI BslI BspMI BssKI BstNI
BstUI Cac8I CfoI DdeI EcoRII
FauI Fnu4HI HaeIII HhaI HinP1I
ItaI MaeII MaeIII MnlI MseI
MspA1I MvaI MvnI MwoI NlaIII
PvuII ScrFI SfcI Tru9I TseI
Tsp45I Tsp509I TspRI