##################################
# NO CUT ENZYMES ( 131 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcsI AgeI AhdI AocI Aor51HI
ApaI ApoI AscI AseI AsnI
Asp718I AspEI AviII AvrII BalI
BamHI BanII BbrPI BcgI BclI
BglI BglII BlnI BsaAI BsaBI
BsaWI BseAI BseRI BsgI BsiEI
BsiWI BsmI BspDI BspEI BspHI
BsrBI BsrFI BssHII BssSI BstBI
BstEII BstPI BstUI BstYI Bsu36I
Cfr10I ClaI CpoI DraII DraIII
DrdI DsaI EaeI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoT14I
FbaI FseI FspI HindIII KpnI
Ksp632I KspI MamI MfeI MflI
MluI MluNI MroI MscI MslI
MspA1I MunI MvnI NaeI NcoI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI PvuII RcaI RsrII SacI
SacII SalI SapI SbfI SfaNI
SfiI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I StuI StyI
SwaI XbaI XhoI XhoII XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 63 enzymes) #
###################################
AflII 636 CTTAAG C^TTAAG
AflIII 228 ACATGT A^C[GA][CT]GT
Alw44I 766 GTGCAC G^TGCAC
AlwNI 260 CAGAATCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 766 GTGCAC G^TGCAC
Asp700I 319 GAACTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 766 GTGCAC G[AT]GC[AT]^C
AspI 735 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 837 CCCGAG C^[CT]CG[GA]G
AvaII 61 GGTCC G^G[AT]CC
BanI 857 GGCGCC G^G[CT][GA]CC
BbeI 857 GGCGCC GGCGC^C
BbvCI 828 CCTCAGC CCTCAGC(-5/-2)
BbvI 1179 GCAGC GCAGC(8/12)
BciVI 27 GGATAC GTATCC(6/5)
BfaI 392 CTAG C^TAG
BfrI 636 CTTAAG C^TTAAG
BlpI 1337 GCTGAGC GC^T[ACGT]AGC
BpmI 761 CTGGAG CTGGAG(16/14)
Bpu1102I 1337 GCTGAGC GC^T[ACGT]AGC
BsiHKAI 766 GTGCAC G[AT]GC[AT]^C
BsiYI 51 CCATGCATTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 51 CCATGCATTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI 848 GGGAC GGGAC(10/14)
BsoBI 837 CCCGAG C^[CT]CG[GA]G
Bsp1407I 420 TGTACA T^GTACA
BspLU11I 228 ACATGT A^CATGT
BspMI 34 GCAGGT ACCTGC(4/8)
BsrGI 420 TGTACA T^GTACA
Bst1107I 1322 GTATAC GTA^TAC
BstAPI 1275 GCAGGGTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I 1322 GTATAC GTA^TAC
CelII 1337 GCTGAGC GC^T[ACGT]AGC
DraI 606 TTTAAA TTT^AAA
EcoRV 1254 GATATC GAT^ATC
FauI 958 CCCGC CCCGC(4/6)
Fnu4HI 1179 GCAGC GC^[ACGT]GC
HaeII 857 GGCGCC [GA]GCGC^[CT]
HgaI 869 GACGC GACGC(5/10)
HincII 1021 GTTAAC GT[CT]^[GA]AC
HindII 1021 GTTAAC GT[CT]^[GA]AC
HpaI 1021 GTTAAC GTT^AAC
ItaI 1179 GCAGC GC^[ACGT]GC
KasI 857 GGCGCC G^GCGCC
MaeI 392 CTAG C^TAG
MaeII 614 ACGT A^CGT
NarI 857 GGCGCC GG^CGCC
NdeI 21 CATATG CA^TATG
PflFI 735 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 51 CCATGCATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 626 CTGCAG CTGCA^G
ScaI 1263 AGTACT AGT^ACT
SexAI 913 ACCAGGT A^CC[AT]GGT
SfoI 857 GGCGCC GGC^GCC
SspBI 420 TGTACA T^GTACA
SspI 1373 AATATT AAT^ATT
TaqI 934 TCGA T^CGA
TseI 1179 GCAGC G^C[AT]GC
Tth111I 735 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 934 TCGA T^CGA
Van91I 51 CCATGCATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 450 CCAGACCAGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 319 GAACTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccI 1300 GTCTAC GT^[AC][GT]AC
1322 GTATAC
AciI 955 CCGC CCGC(-3/-1)
959 CCGC
AcyI 857 GGCGCC G[GA]^CG[CT]C
869 GACGCC
AfaI 421 GTAC GT^AC
1264 GTAC
AlwI 952 GATCC GGATC(4/5)
527 GGATC
BbsI 546 GAAGAC GAAGAC(2/6)
1105 GTCTTC
BmyI 766 GTGCAC G[AGT]GC[ACT]^C
1004 GTGCCC
BpuAI 546 GAAGAC GAAGAC(2/6)
1105 GTCTTC
BsaHI 857 GGCGCC G[GA]^CG[CT]C
869 GACGCC
BsaI 631 GAGACC GGTCTC(1/5)
930 GGTCTC
BsmBI 733 GAGACG CGTCTC(1/5)
900 GAGACG
Bsp1286I 766 GTGCAC G[AGT]GC[ACT]^C
1004 GTGCCC
BsrDI 531 CATTGC GCAATG(2/0)
383 GCAATG
Cac8I 792 GCAAGC GC[ACGT]^[ACGT]GC
957 GCCCGC
CfoI 858 GCGC GCG^C
1395 GCGC
Cfr13I 61 GGTCC G^G[ACGT]CC
1289 GGCCC
EcoT22I 53 ATGCAT ATGCA^T
1079 ATGCAT
HhaI 858 GCGC GCG^C
1395 GCGC
Hin1I 857 GGCGCC G[GA]^CG[CT]C
869 GACGCC
HinP1I 858 GCGC G^CGC
1395 GCGC
HphI 752 TCACC GGTGA(8/7)
911 TCACC
MwoI 1275 GCAGGGTTTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
1395 GCGCAAGGTGC
NlaIV 60 GGGTCC GG[ACGT]^[ACGT]CC
857 GGCGCC
NsiI 53 ATGCAT ATGCA^T
1079 ATGCAT
NspI 228 ACATGT [GA]CATG^[CT]
1081 GCATGT
PleI 250 GAGTC GAGTC(4/5)
739 GAGTC
RsaI 421 GTAC GT^AC
1264 GTAC
Sau96I 61 GGTCC G^G[ACGT]CC
1289 GGCCC
SmlI 443 CTCAAG C^T[CT][GA]AG
636 CTTAAG
#########################################
# 3 OR MORE CUT ENZYMES ( 35 enzymes) #
#########################################
AluI BcnI BsaJI BsmAI BsrI
BssKI BstF5I BstNI BstXI DdeI
DpnII EcoRII FokI HaeIII HapII
HinfI HpaII MaeIII MboI MboII
MnlI MseI MspI MvaI NciI
NdeII NlaIII Sau3AI ScrFI SfcI
TfiI Tru9I Tsp45I Tsp509I TspRI