##################################
# NO CUT ENZYMES ( 138 enzymes) #
##################################
Acc65I AccII AccIII AclI AflII
AflIII AgeI AhdI Alw44I AlwNI
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspEI AvaI
AviII AvrII BamHI BanII BbeI
BbrPI BbvCI BcgI BciVI BclI
BfrI BglI BglII BlnI BlpI
Bpu1102I BsaAI BsaBI BsaI BseAI
BsiEI BsiWI BsmFI BsmI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrDI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstXI BstZ17I Bsu36I CelII
ClaI CpoI DraI DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FauI FbaI FseI FspI
HaeII HpaI KasI KpnI KspI
MamI MluI MroI MvnI NaeI
NarI NdeI NgoMIV NotI NruI
NsiI NspI NspV PacI PaeR7I
PflMI PinAI PmaCI PmeI PmlI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI StuI SwaI Van91I XbaI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AatII 1082 GACGTC GACGT^C
AccI 1044 GTCTAC GT^[AC][GT]AC
AcsI 1184 AAATTT [GA]^AATT[CT]
AcyI 1082 GACGTC G[GA]^CG[CT]C
ApoI 1184 AAATTT [GA]^AATT[CT]
Asp700I 50 GAATGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 763 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanI 115 GGCACC G^G[CT][GA]CC
BbsI 280 GAAGAC GAAGAC(2/6)
BcnI 747 CCCGG CC^[GC]GG
BpuAI 280 GAAGAC GAAGAC(2/6)
BsaHI 1082 GACGTC G[GA]^CG[CT]C
BsaWI 644 ACCGGA [AT]^CCGG[AT]
BsgI 892 GTGCAG GTGCAG(16/14)
BsmBI 1084 CGTCTC CGTCTC(1/5)
BsrFI 1093 GCCGGT [GA]^CCGG[CT]
Cfr10I 1093 GCCGGT [GA]^CCGG[CT]
DraII 761 AGGACCC [GA]G^G[ACGT]CC[CT]
DsaI 500 CCATGG C^C[GA][CT]GG
EarI 273 GAAGAG CTCTTC(1/4)
EcoO109I 761 AGGACCC [GA]G^G[ACGT]CC[CT]
HgaI 1073 GACGC GACGC(5/10)
Hin1I 1082 GACGTC G[GA]^CG[CT]C
Ksp632I 273 GAAGAG CTCTTC(1/4)
MfeI 370 CAATTG C^AATTG
MslI 537 CATTGGGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 370 CAATTG C^AATTG
NciI 747 CCCGG CC^[GC]GG
NcoI 500 CCATGG C^CATGG
NheI 985 GCTAGC G^CTAGC
PflFI 763 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 761 AGGACCC [GA]G^G[AT]CC[CT]
PshAI 1140 GACTTCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SexAI 617 ACCAGGT A^CC[AT]GGT
SmlI 595 CTCAAG C^T[CT][GA]AG
Tth111I 763 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 828 CCAGCTGCAGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 50 GAATGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AciI 189 CCGC CCGC(-3/-1)
558 CCGC
AfaI 485 GTAC GT^AC
600 GTAC
AspHI 701 GTGCTC G[AT]GC[AT]^C
751 GTGCTC
BmyI 701 GTGCTC G[AGT]GC[ACT]^C
751 GTGCTC
BpmI 128 CTCCAG CTGGAG(16/14)
568 CTGGAG
BseRI 5 GAGGAG GAGGAG(10/8)
223 GAGGAG
BsiHKAI 701 GTGCTC G[AT]GC[AT]^C
751 GTGCTC
Bsp1286I 701 GTGCTC G[AGT]GC[ACT]^C
751 GTGCTC
BspMI 228 GCAGGT ACCTGC(4/8)
894 GCAGGT
BstYI 477 GGATCT [GA]^GATC[CT]
709 GGATCT
Cac8I 560 GCAGGC GC[ACGT]^[ACGT]GC
985 GCTAGC
CfoI 822 GCGC GCG^C
876 GCGC
DdeI 3 CTGAG C^T[ACGT]AG
1158 CTGAG
HhaI 822 GCGC GCG^C
876 GCGC
HinP1I 822 GCGC G^CGC
876 GCGC
HindIII 149 AAGCTT A^AGCTT
301 AAGCTT
MaeII 890 ACGT A^CGT
1083 ACGT
MboII 273 GAAGA GAAGA(8/7)
280 GAAGA
MflI 477 GGATCT [GA]^GATC[CT]
709 GGATCT
PleI 593 GACTC GAGTC(4/5)
1160 GAGTC
PstI 832 CTGCAG CTGCA^G
917 CTGCAG
RsaI 485 GTAC GT^AC
600 GTAC
SfaNI 517 GATGC GCATC(5/9)
878 GCATC
SspI 462 AATATT AAT^ATT
1016 AATATT
TaqI 321 TCGA T^CGA
673 TCGA
TfiI 55 GATTC G^A[AT]TC
199 GATTC
TthHB8I 321 TCGA T^CGA
673 TCGA
XhoII 477 GGATCT [GA]^GATC[CT]
709 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 54 enzymes) #
#########################################
AluI AlwI AvaII BalI BbvI
BfaI BsaJI BsiYI BslI BsmAI
BsrI BssKI BstF5I BstNI Cfr13I
DpnII EaeI EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HincII HindII
HinfI HpaII HphI ItaI MaeI
MaeIII MboI MluNI MnlI MscI
MseI MspA1I MspI MvaI MwoI
NdeII NlaIII NlaIV PvuII Sau3AI
Sau96I ScrFI SfcI StyI Tru9I
TseI Tsp45I Tsp509I TspRI