##################################
# NO CUT ENZYMES ( 121 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AhdI Alw44I
AocI ApaLI AscI Asp700I Asp718I
AspEI AspI AviII AvrII BbeI
BbrPI BbsI BbvCI BcgI BciVI
BclI BfaI BfrI BlnI BpuAI
BsaAI BsaI BsaWI BseAI BsiEI
BsiWI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrDI BsrGI
BssHII Bst1107I BstBI BstEII BstPI
BstZ17I Bsu36I ClaI CpoI DrdI
EagI Eam1105I EclXI Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FbaI FspI HincII HindII HindIII
HpaI KasI KpnI MaeI MfeI
MluI MroI MunI NarI NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI Psp1406I PvuI
RcaI RsrII SalI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI TaqI Tth111I TthHB8I
XbaI XcmI XhoI XmaCI XmaI
XmnI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AcyI 622 GGCGTC G[GA]^CG[CT]C
AfaI 280 GTAC GT^AC
Aor51HI 375 AGCGCT AGC^GCT
ApaI 1217 GGGCCC GGGCC^C
AseI 269 ATTAAT AT^TAAT
AsnI 269 ATTAAT AT^TAAT
BalI 75 TGGCCA TGG^CCA
BamHI 511 GGATCC G^GATCC
BcnI 106 CCGGG CC^[GC]GG
BglI 1219 GCCCTCGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 371 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 371 GCTGAGC GC^T[ACGT]AGC
BsaHI 622 GGCGTC G[GA]^CG[CT]C
BseRI 1135 GAGGAG GAGGAG(10/8)
BsmBI 624 CGTCTC CGTCTC(1/5)
BspMI 88 ACCTGC ACCTGC(4/8)
BsrFI 201 GCCGGC [GA]^CCGG[CT]
BssSI 1222 CTCGTG CACGAG(-5/-1)
BstAPI 1147 GCAGCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 371 GCTGAGC GC^T[ACGT]AGC
Cfr10I 201 GCCGGC [GA]^CCGG[CT]
DraI 1335 TTTAAA TTT^AAA
DraIII 178 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 75 TGGCCA [CT]^GGCC[GA]
Eco47III 375 AGCGCT AGC^GCT
FseI 200 GGCCGGCC GGCCGG^CC
HaeII 375 AGCGCT [GA]GCGC^[CT]
Hin1I 622 GGCGTC G[GA]^CG[CT]C
KspI 616 CCGCGG CCGC^GG
MaeII 588 ACGT A^CGT
MluNI 75 TGGCCA TGG^CCA
MscI 75 TGGCCA TGG^CCA
NaeI 201 GCCGGC GCC^GGC
NciI 106 CCGGG CC^[GC]GG
NdeI 93 CATATG CA^TATG
NgoMIV 201 GCCGGC G^CCGGC
PpuMI 85 GGGACCT [GA]G^G[AT]CC[CT]
PshBI 269 ATTAAT AT^TAAT
PstI 1113 CTGCAG CTGCA^G
RsaI 280 GTAC GT^AC
SacII 616 CCGCGG CCGC^GG
SapI 499 GCTCTTC GCTCTTC(1/4)
SfcI 1113 CTGCAG C^T[GA][CT]AG
SwaI 1334 ATTTAAAT ATTT^AAAT
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccII 218 CGCG CG^CG
617 CGCG
AcsI 922 AAATTT [GA]^AATT[CT]
1345 GAATTT
ApoI 922 AAATTT [GA]^AATT[CT]
1345 GAATTT
AvaI 284 CCCGAG C^[CT]CG[GA]G
486 CCCGAG
BanI 608 GGCACC G^G[CT][GA]CC
822 GGTGCC
BglII 145 AGATCT A^GATCT
348 AGATCT
BpmI 28 CTCCAG CTGGAG(16/14)
149 CTGGAG
BsaBI 140 GATGCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
157 GATCCTGATC
BsoBI 284 CCCGAG C^[CT]CG[GA]G
486 CCCGAG
BstUI 218 CGCG CG^CG
617 CGCG
CfoI 20 GCGC GCG^C
376 GCGC
EarI 500 CTCTTC CTCTTC(1/4)
900 CTCTTC
HapII 106 CCGG C^CGG
202 CCGG
HhaI 20 GCGC GCG^C
376 GCGC
HinP1I 20 GCGC G^CGC
376 GCGC
HpaII 106 CCGG C^CGG
202 CCGG
Ksp632I 500 CTCTTC CTCTTC(1/4)
900 CTCTTC
MamI 140 GATGCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
157 GATCCTGATC
MspI 106 CCGG C^CGG
202 CCGG
MvnI 218 CGCG CG^CG
617 CGCG
PleI 12 GAGTC GAGTC(4/5)
1036 GAGTC
StuI 315 AGGCCT AGG^CCT
683 AGGCCT
TfiI 463 GAATC G^A[AT]TC
544 GAATC
#########################################
# 3 OR MORE CUT ENZYMES ( 70 enzymes) #
#########################################
AciI AluI AlwI AlwNI AspHI
AvaII BanII BbvI BmyI BsaJI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI BstXI BstYI Cac8I Cfr13I
DdeI DpnII DraII DsaI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HgaI HinfI HphI ItaI
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MvaI MwoI
NcoI NdeII NlaIII NlaIV PflMI
PvuII SacI Sau3AI Sau96I ScrFI
SfaNI SmlI StyI Tru9I TseI
Tsp45I Tsp509I TspRI Van91I XhoII